Advanced

Characterization of Meteorin-An Evolutionary Conserved Neurotrophic Factor

Jorgensen, Jesper Roland; Thompson, Lachlan LU ; Fjord-Larsen, Lone; Krabbe, Christina; Torp, Malene; Kalkkinen, Nisse; Hansen, Claus and Wahlberg, Lars (2009) In Journal of Molecular Neuroscience 39(1-2). p.104-116
Abstract
Growth factors control cellular growth, proliferation, and differentiation and may have therapeutic applications. In this study, we focus on Meteorin which is a member of a largely uncharacterized evolutionary conserved two-member growth factor family. Our analysis shows that Meteorin is expressed in the central nervous system both during development and in adult mice. Detailed immunohistological analysis of the adult mouse brain reveals that Meteorin is highly expressed in Bergmann glia and in a few discrete neuronal populations residing in the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and in various thalamic nuclei. In addition, low levels of Meteorin is found in astrocytes (S100 beta+, OX42-)... (More)
Growth factors control cellular growth, proliferation, and differentiation and may have therapeutic applications. In this study, we focus on Meteorin which is a member of a largely uncharacterized evolutionary conserved two-member growth factor family. Our analysis shows that Meteorin is expressed in the central nervous system both during development and in adult mice. Detailed immunohistological analysis of the adult mouse brain reveals that Meteorin is highly expressed in Bergmann glia and in a few discrete neuronal populations residing in the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and in various thalamic nuclei. In addition, low levels of Meteorin is found in astrocytes (S100 beta+, OX42-) distributed ubiquitously throughout the brain. Meteorin was cloned and recombinant protein purified allowing N-terminal sequencing and mass spectrometric analysis showing that Meteorin is secreted as an unmodified monomer. This form is bioactive as it induces neurite outgrowth from dorsal root ganglions in vitro. Intrastriatal protein injection and lentiviral studies in vivo showed that Meteorin is a highly diffusible molecule in the brain and cellular uptake is apparent in specific populations which may carry the receptor. In summary, we provide a comprehensive expression analysis and have made and thoroughly validated molecular tools to help investigate the therapeutic potential of Meteorin. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gene therapy, Expression, Growth factor, Biologics, In vivo delivery
in
Journal of Molecular Neuroscience
volume
39
issue
1-2
pages
104 - 116
publisher
Humana Press
external identifiers
  • wos:000269903800012
  • scopus:74149093893
ISSN
0895-8696
DOI
10.1007/s12031-009-9189-4
language
English
LU publication?
yes
id
396ab881-2963-48c6-a9d5-e265d72c4010 (old id 1490672)
date added to LUP
2009-10-19 13:45:56
date last changed
2017-10-01 04:03:14
@article{396ab881-2963-48c6-a9d5-e265d72c4010,
  abstract     = {Growth factors control cellular growth, proliferation, and differentiation and may have therapeutic applications. In this study, we focus on Meteorin which is a member of a largely uncharacterized evolutionary conserved two-member growth factor family. Our analysis shows that Meteorin is expressed in the central nervous system both during development and in adult mice. Detailed immunohistological analysis of the adult mouse brain reveals that Meteorin is highly expressed in Bergmann glia and in a few discrete neuronal populations residing in the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and in various thalamic nuclei. In addition, low levels of Meteorin is found in astrocytes (S100 beta+, OX42-) distributed ubiquitously throughout the brain. Meteorin was cloned and recombinant protein purified allowing N-terminal sequencing and mass spectrometric analysis showing that Meteorin is secreted as an unmodified monomer. This form is bioactive as it induces neurite outgrowth from dorsal root ganglions in vitro. Intrastriatal protein injection and lentiviral studies in vivo showed that Meteorin is a highly diffusible molecule in the brain and cellular uptake is apparent in specific populations which may carry the receptor. In summary, we provide a comprehensive expression analysis and have made and thoroughly validated molecular tools to help investigate the therapeutic potential of Meteorin.},
  author       = {Jorgensen, Jesper Roland and Thompson, Lachlan and Fjord-Larsen, Lone and Krabbe, Christina and Torp, Malene and Kalkkinen, Nisse and Hansen, Claus and Wahlberg, Lars},
  issn         = {0895-8696},
  keyword      = {Gene therapy,Expression,Growth factor,Biologics,In vivo delivery},
  language     = {eng},
  number       = {1-2},
  pages        = {104--116},
  publisher    = {Humana Press},
  series       = {Journal of Molecular Neuroscience},
  title        = {Characterization of Meteorin-An Evolutionary Conserved Neurotrophic Factor},
  url          = {http://dx.doi.org/10.1007/s12031-009-9189-4},
  volume       = {39},
  year         = {2009},
}