Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modification
(2009) In Bioelectrochemistry 76(1-2). p.87-92- Abstract
- Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron... (More)
- Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron transfer with that obtained through mediated electron transfer. it was clearly observed that the improved current of the SPE vertical bar PANI vertical bar CDH electrode is due to the specific role of PANI, rather than caused by a rise of enzyme loading. The operational stability of the enzyme electrode based on PANI modified SPE was 5 times higher compared with that based on plain SPE. (C) 2009 Elsevier B.V. All rights reserved (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1490776
- author
- Trashin, Stanislav A. ; Haltrich, Dietmar ; Ludwig, Roland ; Gorton, Lo LU and Karyakin, Arkady A.
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lactose, CDH, Polyaniline, Direct bioelectrocatalysis, Biosensor
- in
- Bioelectrochemistry
- volume
- 76
- issue
- 1-2
- pages
- 87 - 92
- publisher
- Elsevier
- external identifiers
-
- wos:000270014400015
- scopus:68549101533
- ISSN
- 1878-562X
- DOI
- 10.1016/j.bioelechem.2009.06.004
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
- id
- 9f406d01-d803-4bbc-ad01-4e0d5c512130 (old id 1490776)
- date added to LUP
- 2016-04-01 11:40:05
- date last changed
- 2022-01-26 08:28:03
@article{9f406d01-d803-4bbc-ad01-4e0d5c512130, abstract = {{Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron transfer with that obtained through mediated electron transfer. it was clearly observed that the improved current of the SPE vertical bar PANI vertical bar CDH electrode is due to the specific role of PANI, rather than caused by a rise of enzyme loading. The operational stability of the enzyme electrode based on PANI modified SPE was 5 times higher compared with that based on plain SPE. (C) 2009 Elsevier B.V. All rights reserved}}, author = {{Trashin, Stanislav A. and Haltrich, Dietmar and Ludwig, Roland and Gorton, Lo and Karyakin, Arkady A.}}, issn = {{1878-562X}}, keywords = {{Lactose; CDH; Polyaniline; Direct bioelectrocatalysis; Biosensor}}, language = {{eng}}, number = {{1-2}}, pages = {{87--92}}, publisher = {{Elsevier}}, series = {{Bioelectrochemistry}}, title = {{Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modification}}, url = {{http://dx.doi.org/10.1016/j.bioelechem.2009.06.004}}, doi = {{10.1016/j.bioelechem.2009.06.004}}, volume = {{76}}, year = {{2009}}, }