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Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modification

Trashin, Stanislav A. ; Haltrich, Dietmar ; Ludwig, Roland ; Gorton, Lo LU and Karyakin, Arkady A. (2009) In Bioelectrochemistry 76(1-2). p.87-92
Abstract
Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron... (More)
Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron transfer with that obtained through mediated electron transfer. it was clearly observed that the improved current of the SPE vertical bar PANI vertical bar CDH electrode is due to the specific role of PANI, rather than caused by a rise of enzyme loading. The operational stability of the enzyme electrode based on PANI modified SPE was 5 times higher compared with that based on plain SPE. (C) 2009 Elsevier B.V. All rights reserved (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lactose, CDH, Polyaniline, Direct bioelectrocatalysis, Biosensor
in
Bioelectrochemistry
volume
76
issue
1-2
pages
87 - 92
publisher
Elsevier
external identifiers
  • wos:000270014400015
  • scopus:68549101533
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2009.06.004
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
id
9f406d01-d803-4bbc-ad01-4e0d5c512130 (old id 1490776)
date added to LUP
2016-04-01 11:40:05
date last changed
2022-01-26 08:28:03
@article{9f406d01-d803-4bbc-ad01-4e0d5c512130,
  abstract     = {{Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron transfer with that obtained through mediated electron transfer. it was clearly observed that the improved current of the SPE vertical bar PANI vertical bar CDH electrode is due to the specific role of PANI, rather than caused by a rise of enzyme loading. The operational stability of the enzyme electrode based on PANI modified SPE was 5 times higher compared with that based on plain SPE. (C) 2009 Elsevier B.V. All rights reserved}},
  author       = {{Trashin, Stanislav A. and Haltrich, Dietmar and Ludwig, Roland and Gorton, Lo and Karyakin, Arkady A.}},
  issn         = {{1878-562X}},
  keywords     = {{Lactose; CDH; Polyaniline; Direct bioelectrocatalysis; Biosensor}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{87--92}},
  publisher    = {{Elsevier}},
  series       = {{Bioelectrochemistry}},
  title        = {{Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modification}},
  url          = {{http://dx.doi.org/10.1016/j.bioelechem.2009.06.004}},
  doi          = {{10.1016/j.bioelechem.2009.06.004}},
  volume       = {{76}},
  year         = {{2009}},
}