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Growth of inoculated white-rot fungi and their interactions with the bacterial community in soil contaminated with polycyclic aromatic hydrocarbons, as measured by phospholipid fatty acids

Andersson, B E; Welinder, L; Olsson, P A; Henrysson, Tomas LU and Olsson, S (2000) In Bioresource Technology 73(1). p.29-36
Abstract
The objective of this study was to examine the possibility of measuring the growth of three white-rot fungi in soil contaminated with polycyclic aromatic hydrocarbons (PAHs), by estimating the soil levels of the phospholipid fatty acid (PLFA) 18:2ω6,9. The effect of the fungi on the PAH concentration and on the indigenous bacterial population in the soil was monitored. As shown by visual examination, the fungi investigated, Pleurotus ostreatus, Phanerochaete chrysoporium and Hypholoma fasciculare, grew well in autoclaved soil, whilst only H. fasciculare grew in non-autoclaved soil. In these reactors, there was also detected an increase in the PLFA 18:2ω6,9. However, the interpretation of the PLFA data was somewhat disturbed since 18:2ω6,9... (More)
The objective of this study was to examine the possibility of measuring the growth of three white-rot fungi in soil contaminated with polycyclic aromatic hydrocarbons (PAHs), by estimating the soil levels of the phospholipid fatty acid (PLFA) 18:2ω6,9. The effect of the fungi on the PAH concentration and on the indigenous bacterial population in the soil was monitored. As shown by visual examination, the fungi investigated, Pleurotus ostreatus, Phanerochaete chrysoporium and Hypholoma fasciculare, grew well in autoclaved soil, whilst only H. fasciculare grew in non-autoclaved soil. In these reactors, there was also detected an increase in the PLFA 18:2ω6,9. However, the interpretation of the PLFA data was somewhat disturbed since 18:2ω6,9 also was found to be present in the birch wood used as a fungal substrate. In autoclaved soil, P. ostreatus and P. chrysosporium were found to exhibit a PAH-degrading capability, with the total PAH concentration decreasing from 209±35 and 186±2 to 149±6 and 109±6 mg/kg dry weight (dw) soil, respectively, during the 10 week incubation period. No PAH-degradation could be detected in any treatment using non-autoclaved soil. In the autoclaved soil, the total level of bacterial specific PLFAs in all fungal treatments, and in a control using added ground birch sticks, was found to be lowered. In the non-autoclaved soil, 6 out of 9 selected bacterial PLFAs exhibited a significant change between the treatments, but the overall total content of bacterial PLFAs did not change. The present study has shown that it is possible to measure fungal growth in a PAH-contaminated soil derived from a former gasworks plant by estimating the levels of the PLFA 18:2ω6,9. The inoculated fungi affected the indigenous bacteria, as shown by estimating the level of bacterial specific PLFAs. Finally, fungal PAH-degradation could be detected in autoclaved soil but not in non-autoclaved soil. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioresource Technology
volume
73
issue
1
pages
29 - 36
publisher
Elsevier
external identifiers
  • scopus:0034190795
ISSN
1873-2976
DOI
10.1016/S0960-8524(99)00134-0
language
English
LU publication?
yes
id
a180ac21-bfda-4bd9-a41f-0d395672606a (old id 149440)
date added to LUP
2007-06-29 08:52:22
date last changed
2017-07-30 04:29:13
@article{a180ac21-bfda-4bd9-a41f-0d395672606a,
  abstract     = {The objective of this study was to examine the possibility of measuring the growth of three white-rot fungi in soil contaminated with polycyclic aromatic hydrocarbons (PAHs), by estimating the soil levels of the phospholipid fatty acid (PLFA) 18:2ω6,9. The effect of the fungi on the PAH concentration and on the indigenous bacterial population in the soil was monitored. As shown by visual examination, the fungi investigated, Pleurotus ostreatus, Phanerochaete chrysoporium and Hypholoma fasciculare, grew well in autoclaved soil, whilst only H. fasciculare grew in non-autoclaved soil. In these reactors, there was also detected an increase in the PLFA 18:2ω6,9. However, the interpretation of the PLFA data was somewhat disturbed since 18:2ω6,9 also was found to be present in the birch wood used as a fungal substrate. In autoclaved soil, P. ostreatus and P. chrysosporium were found to exhibit a PAH-degrading capability, with the total PAH concentration decreasing from 209±35 and 186±2 to 149±6 and 109±6 mg/kg dry weight (dw) soil, respectively, during the 10 week incubation period. No PAH-degradation could be detected in any treatment using non-autoclaved soil. In the autoclaved soil, the total level of bacterial specific PLFAs in all fungal treatments, and in a control using added ground birch sticks, was found to be lowered. In the non-autoclaved soil, 6 out of 9 selected bacterial PLFAs exhibited a significant change between the treatments, but the overall total content of bacterial PLFAs did not change. The present study has shown that it is possible to measure fungal growth in a PAH-contaminated soil derived from a former gasworks plant by estimating the levels of the PLFA 18:2ω6,9. The inoculated fungi affected the indigenous bacteria, as shown by estimating the level of bacterial specific PLFAs. Finally, fungal PAH-degradation could be detected in autoclaved soil but not in non-autoclaved soil.},
  author       = {Andersson, B E and Welinder, L and Olsson, P A and Henrysson, Tomas and Olsson, S},
  issn         = {1873-2976},
  language     = {eng},
  number       = {1},
  pages        = {29--36},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Growth of inoculated white-rot fungi and their interactions with the bacterial community in soil contaminated with polycyclic aromatic hydrocarbons, as measured by phospholipid fatty acids},
  url          = {http://dx.doi.org/10.1016/S0960-8524(99)00134-0},
  volume       = {73},
  year         = {2000},
}