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Seasonal variation of phosphorus limitation of bacterial growth in a small lake

Carlsson, Per LU and Caron, D (2001) In Limnology and Oceanography 46(1). p.108-120
Abstract
A series of bioassay experiments were performed from spring to autumn in a small dimictic lake (Deep Pond,

Massachusetts) to examine the potential for bacterial growth limitation by organic carbon (glucose), inorganic

nutrients (ammonium or phosphate), or both. The experiments demonstrated that phosphorus was the primary

element limiting bacterial growth in Deep Pond during a large part of the summer. Significant increases (relative

to controls) in bacterial cell volumes, protein production rates, and abundances were observed during 24-h incubations

for samples amended with phosphate alone. Organic carbon was near colimitation for most of the samples,

however, and dramatic increases in... (More)
A series of bioassay experiments were performed from spring to autumn in a small dimictic lake (Deep Pond,

Massachusetts) to examine the potential for bacterial growth limitation by organic carbon (glucose), inorganic

nutrients (ammonium or phosphate), or both. The experiments demonstrated that phosphorus was the primary

element limiting bacterial growth in Deep Pond during a large part of the summer. Significant increases (relative

to controls) in bacterial cell volumes, protein production rates, and abundances were observed during 24-h incubations

for samples amended with phosphate alone. Organic carbon was near colimitation for most of the samples,

however, and dramatic increases in bacterial abundance and rates of protein production were obtained only when

both substances (phosphate and glucose) were added together. There was no evidence for nitrogen limitation of

bacterial growth during the study. Temperature was not an important determinant for bacterial production rates

above 128C, but below 128C temperature acted to mute the effect of nutrient and organic carbon additions on

production rates. Bacterial growth was not significantly increased by the addition of any combination of glucose,

ammonium, or phosphate below 128C. A significant, albeit complex, effect of the microbial community on the

bacterial response to nutrient/carbon enrichment was apparent in the samples. Substrate/nutrient supply and biomass

removal by bacterivores both appeared to play a role in the outcome of the experiments. (Less)
Please use this url to cite or link to this publication:
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organization
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type
Contribution to journal
publication status
published
subject
in
Limnology and Oceanography
volume
46
issue
1
pages
108 - 120
publisher
ASLO
external identifiers
  • scopus:0035138735
ISSN
1939-5590
language
English
LU publication?
yes
id
525c86d7-09bc-4456-a0db-61068a43aeaf (old id 149841)
alternative location
http://www.aslo.org/lo/toc/vol_46/issue_1/0108.pdf
date added to LUP
2007-06-28 12:44:37
date last changed
2018-10-03 11:34:26
@article{525c86d7-09bc-4456-a0db-61068a43aeaf,
  abstract     = {A series of bioassay experiments were performed from spring to autumn in a small dimictic lake (Deep Pond,<br/><br>
Massachusetts) to examine the potential for bacterial growth limitation by organic carbon (glucose), inorganic<br/><br>
nutrients (ammonium or phosphate), or both. The experiments demonstrated that phosphorus was the primary<br/><br>
element limiting bacterial growth in Deep Pond during a large part of the summer. Significant increases (relative<br/><br>
to controls) in bacterial cell volumes, protein production rates, and abundances were observed during 24-h incubations<br/><br>
for samples amended with phosphate alone. Organic carbon was near colimitation for most of the samples,<br/><br>
however, and dramatic increases in bacterial abundance and rates of protein production were obtained only when<br/><br>
both substances (phosphate and glucose) were added together. There was no evidence for nitrogen limitation of<br/><br>
bacterial growth during the study. Temperature was not an important determinant for bacterial production rates<br/><br>
above 128C, but below 128C temperature acted to mute the effect of nutrient and organic carbon additions on<br/><br>
production rates. Bacterial growth was not significantly increased by the addition of any combination of glucose,<br/><br>
ammonium, or phosphate below 128C. A significant, albeit complex, effect of the microbial community on the<br/><br>
bacterial response to nutrient/carbon enrichment was apparent in the samples. Substrate/nutrient supply and biomass<br/><br>
removal by bacterivores both appeared to play a role in the outcome of the experiments.},
  author       = {Carlsson, Per and Caron, D},
  issn         = {1939-5590},
  language     = {eng},
  number       = {1},
  pages        = {108--120},
  publisher    = {ASLO},
  series       = {Limnology and Oceanography},
  title        = {Seasonal variation of phosphorus limitation of bacterial growth in a small lake},
  volume       = {46},
  year         = {2001},
}