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Different methods for toxin analysis in the cyanobacterium Nodularia spumigena (Cyanophyceae)

Dahlmann, J; Rühl, A; Hummert, C; Liebezeit, G; Carlsson, Per LU and Graneli, E (2001) In Toxicon 39(8). p.1183-1190
Abstract
The brackish water cyanobacterium Nodularia spumigena produce the hepatotoxic cyclic pentapeptide nodularin. Intoxications for both human as well as animal may arise when water reservoirs are contaminated with potentially toxic Nodularia species. Here, results of three independent methods for the determination of nodularin in different strains of N. spumigena are presented. The results obtained with a protein phosphatase assay and a HPLC/UV/MS method are compared with the results obtained with a bioluminescence assay, which is successfully introduced here for nodularin determination. Statistical evaluation of the three applied methods revealed a good comparability towards the detected toxin content. The methods were evaluated taking into... (More)
The brackish water cyanobacterium Nodularia spumigena produce the hepatotoxic cyclic pentapeptide nodularin. Intoxications for both human as well as animal may arise when water reservoirs are contaminated with potentially toxic Nodularia species. Here, results of three independent methods for the determination of nodularin in different strains of N. spumigena are presented. The results obtained with a protein phosphatase assay and a HPLC/UV/MS method are compared with the results obtained with a bioluminescence assay, which is successfully introduced here for nodularin determination. Statistical evaluation of the three applied methods revealed a good comparability towards the detected toxin content. The methods were evaluated taking into consideration the parameters: handling, efficiency, sensitivity and selectivity. The detection limit in the protein phosphatase assay is highest (0.05 ng nodularin) and lowest (250 ng nodularin) in the bioluminescence assay— it was determined with 5 ng (MS) and 25 ng (UV) for the HPLC/UV/MS methods. The different selectivities and sensitivities are critically discussed and an analytical pathway for the determination of the biotoxin nodularin from Nodularia samples is proposed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Toxicon
volume
39
issue
8
pages
1183 - 1190
publisher
Elsevier
external identifiers
  • scopus:0035075364
ISSN
0041-0101
DOI
10.1016/S0041-0101(00)00256-7
language
English
LU publication?
yes
id
d32852e2-f8d8-47fe-86f5-019ef5a76f6a (old id 149845)
date added to LUP
2007-06-28 12:57:30
date last changed
2018-05-29 09:32:31
@article{d32852e2-f8d8-47fe-86f5-019ef5a76f6a,
  abstract     = {The brackish water cyanobacterium Nodularia spumigena produce the hepatotoxic cyclic pentapeptide nodularin. Intoxications for both human as well as animal may arise when water reservoirs are contaminated with potentially toxic Nodularia species. Here, results of three independent methods for the determination of nodularin in different strains of N. spumigena are presented. The results obtained with a protein phosphatase assay and a HPLC/UV/MS method are compared with the results obtained with a bioluminescence assay, which is successfully introduced here for nodularin determination. Statistical evaluation of the three applied methods revealed a good comparability towards the detected toxin content. The methods were evaluated taking into consideration the parameters: handling, efficiency, sensitivity and selectivity. The detection limit in the protein phosphatase assay is highest (0.05 ng nodularin) and lowest (250 ng nodularin) in the bioluminescence assay— it was determined with 5 ng (MS) and 25 ng (UV) for the HPLC/UV/MS methods. The different selectivities and sensitivities are critically discussed and an analytical pathway for the determination of the biotoxin nodularin from Nodularia samples is proposed.},
  author       = {Dahlmann, J and Rühl, A and Hummert, C and Liebezeit, G and Carlsson, Per and Graneli, E},
  issn         = {0041-0101},
  language     = {eng},
  number       = {8},
  pages        = {1183--1190},
  publisher    = {Elsevier},
  series       = {Toxicon},
  title        = {Different methods for toxin analysis in the cyanobacterium Nodularia spumigena (Cyanophyceae)},
  url          = {http://dx.doi.org/10.1016/S0041-0101(00)00256-7},
  volume       = {39},
  year         = {2001},
}