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W41/W41 blastocyst complementation: a system for genetic modeling of hematopoiesis.

Jansson, Lina LU and Larsson, Jonas LU (2010) In Blood 115(1). p.47-50
Abstract
We report a rapid and highly efficient approach to generate mice in which the hematopoietic system is derived from embryonic stem (ES) cells. We show that ES cells injected into blastocysts from the c-kit deficient W41/W41 mouse strain have a clear advantage over the W41/W41 blastocyst-derived inner cell mass cells in founding the hematopoietic system. Fetal liver HSCs from W41/W41 blastocyst complementation embryos can be transplanted to establish large cohorts of bone marrow chimeras with hematopoiesis of practically pure ES cell origin. Using ES cells with site-directed modifications we show how this system can be employed to drive inducible transgene expression in hematopoietic cells in a robust and reliable manner both in vitro and in... (More)
We report a rapid and highly efficient approach to generate mice in which the hematopoietic system is derived from embryonic stem (ES) cells. We show that ES cells injected into blastocysts from the c-kit deficient W41/W41 mouse strain have a clear advantage over the W41/W41 blastocyst-derived inner cell mass cells in founding the hematopoietic system. Fetal liver HSCs from W41/W41 blastocyst complementation embryos can be transplanted to establish large cohorts of bone marrow chimeras with hematopoiesis of practically pure ES cell origin. Using ES cells with site-directed modifications we show how this system can be employed to drive inducible transgene expression in hematopoietic cells in a robust and reliable manner both in vitro and in vivo. The approach avoids the cost and time constraints associated with the creation of standard transgenic mouse strains while taking advantage of the sophisticated site-directed manipulations that are possible in ES cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
115
issue
1
pages
47 - 50
publisher
American Society of Hematology
external identifiers
  • wos:000273389600011
  • pmid:19864644
  • scopus:74949125790
ISSN
1528-0020
DOI
10.1182/blood-2009-07-235622
language
English
LU publication?
yes
id
531df94f-354d-46df-a190-7c00d991a676 (old id 1499893)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19864644?dopt=Abstract
date added to LUP
2009-11-03 09:26:51
date last changed
2018-05-29 12:19:03
@article{531df94f-354d-46df-a190-7c00d991a676,
  abstract     = {We report a rapid and highly efficient approach to generate mice in which the hematopoietic system is derived from embryonic stem (ES) cells. We show that ES cells injected into blastocysts from the c-kit deficient W41/W41 mouse strain have a clear advantage over the W41/W41 blastocyst-derived inner cell mass cells in founding the hematopoietic system. Fetal liver HSCs from W41/W41 blastocyst complementation embryos can be transplanted to establish large cohorts of bone marrow chimeras with hematopoiesis of practically pure ES cell origin. Using ES cells with site-directed modifications we show how this system can be employed to drive inducible transgene expression in hematopoietic cells in a robust and reliable manner both in vitro and in vivo. The approach avoids the cost and time constraints associated with the creation of standard transgenic mouse strains while taking advantage of the sophisticated site-directed manipulations that are possible in ES cells.},
  author       = {Jansson, Lina and Larsson, Jonas},
  issn         = {1528-0020},
  language     = {eng},
  number       = {1},
  pages        = {47--50},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {W41/W41 blastocyst complementation: a system for genetic modeling of hematopoiesis.},
  url          = {http://dx.doi.org/10.1182/blood-2009-07-235622},
  volume       = {115},
  year         = {2010},
}