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A Novel Voltammetric Point-of-Care Device for Rapid and Accurate Nucleic Acid and Viral Pathogen Detection

Munita, Roberto ; Lyttleton, Roman ; Danefur, Emelie ; Sasinovich, Sviataslau LU ; Wehlin, Karin ; Ruzgas, Tautgirdas ; Medstrand, Patrik LU orcid ; Shin, Jae Yen ; Nyberg, Andreas and Punyani, Kushagr (2026) In ACS Omega 11(23). p.33523-33531
Abstract

With zoonotic outbreaks on the rise, rapid and accurate infectious disease diagnostics are critical for both human and animal health. Traditional lateral flow assays lack sensitivity and specificity, while isothermal amplification methods like LAMP, though rapid, can be hindered by optical detection issues. We present an electrochemical nucleic acid amplification test (NAAT) that combines isothermal amplification with real-time voltammetric detection. This novel method relies on monitoring of amplification-associated proton release through redox probing of a pH-sensing compound. The method shows high concordance with RT-qPCR under the tested conditions and demonstrates a limit of detection (LOD) of 50 copies/reaction for IAV. In RNA... (More)

With zoonotic outbreaks on the rise, rapid and accurate infectious disease diagnostics are critical for both human and animal health. Traditional lateral flow assays lack sensitivity and specificity, while isothermal amplification methods like LAMP, though rapid, can be hindered by optical detection issues. We present an electrochemical nucleic acid amplification test (NAAT) that combines isothermal amplification with real-time voltammetric detection. This novel method relies on monitoring of amplification-associated proton release through redox probing of a pH-sensing compound. The method shows high concordance with RT-qPCR under the tested conditions and demonstrates a limit of detection (LOD) of 50 copies/reaction for IAV. In RNA purified from clinical samples, 231/234 (98.7%) are concordant with RT-qPCR for SARS-CoV-2, IAV, IBV, or RSV A. In a proof-of-concept extraction-free workflow, 14/14 equine nasal swab samples are correctly classified for EHV-5 relative to qPCR. Together, these data support the feasibility of real-time voltammetric LAMP across several sample types, while broader point-of-care validation across additional matrices and prospective cohorts remains to be established.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
ACS Omega
volume
11
issue
23
pages
9 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:42326706
  • scopus:105042123241
ISSN
2470-1343
DOI
10.1021/acsomega.5c12087
language
English
LU publication?
yes
id
14a8d7a6-c7fc-402b-b785-1490abe9497f
date added to LUP
2026-09-02 14:13:49
date last changed
2026-09-16 15:01:17
@article{14a8d7a6-c7fc-402b-b785-1490abe9497f,
  abstract     = {{<p>With zoonotic outbreaks on the rise, rapid and accurate infectious disease diagnostics are critical for both human and animal health. Traditional lateral flow assays lack sensitivity and specificity, while isothermal amplification methods like LAMP, though rapid, can be hindered by optical detection issues. We present an electrochemical nucleic acid amplification test (NAAT) that combines isothermal amplification with real-time voltammetric detection. This novel method relies on monitoring of amplification-associated proton release through redox probing of a pH-sensing compound. The method shows high concordance with RT-qPCR under the tested conditions and demonstrates a limit of detection (LOD) of 50 copies/reaction for IAV. In RNA purified from clinical samples, 231/234 (98.7%) are concordant with RT-qPCR for SARS-CoV-2, IAV, IBV, or RSV A. In a proof-of-concept extraction-free workflow, 14/14 equine nasal swab samples are correctly classified for EHV-5 relative to qPCR. Together, these data support the feasibility of real-time voltammetric LAMP across several sample types, while broader point-of-care validation across additional matrices and prospective cohorts remains to be established.</p>}},
  author       = {{Munita, Roberto and Lyttleton, Roman and Danefur, Emelie and Sasinovich, Sviataslau and Wehlin, Karin and Ruzgas, Tautgirdas and Medstrand, Patrik and Shin, Jae Yen and Nyberg, Andreas and Punyani, Kushagr}},
  issn         = {{2470-1343}},
  language     = {{eng}},
  number       = {{23}},
  pages        = {{33523--33531}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Omega}},
  title        = {{A Novel Voltammetric Point-of-Care Device for Rapid and Accurate Nucleic Acid and Viral Pathogen Detection}},
  url          = {{http://dx.doi.org/10.1021/acsomega.5c12087}},
  doi          = {{10.1021/acsomega.5c12087}},
  volume       = {{11}},
  year         = {{2026}},
}