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Proton NMR of (15)N-Choline Metabolites Enhanced by Dynamic Nuclear Polarization.

Sarkar, Riddhiman; Comment, Arnaud; Vasos, Paul R; Jannin, Sami; Gruetter, Rolf; Bodenhausen, Geoffrey; Hall, Hélène; Kirik, Deniz LU and Denisov, Vladimir LU (2009) In Journal of the American Chemical Society 131(44). p.16014-16014
Abstract
Chemical shifts of protons can report on metabolic transformations such as the conversion of choline to phosphocholine. To follow such processes in vivo, magnetization can be enhanced by dynamic nuclear polarization (DNP). We have hyperpolarized in this manner nitrogen-15 spins in (15)N-labeled choline up to 3.3% by irradiating the 94 GHz electron spin resonance of admixed TEMPO nitroxide radicals in a magnetic field of 3.35 T during ca. 3 h at 1.2 K. The sample was subsequently transferred to a high-resolution magnet, and the enhanced polarization was converted from (15)N to methyl- and methylene protons, using the small (2,3)J((1)H,(15)N) couplings in choline. The room-temperature lifetime of nitrogen polarization in choline, T(1)((15)N)... (More)
Chemical shifts of protons can report on metabolic transformations such as the conversion of choline to phosphocholine. To follow such processes in vivo, magnetization can be enhanced by dynamic nuclear polarization (DNP). We have hyperpolarized in this manner nitrogen-15 spins in (15)N-labeled choline up to 3.3% by irradiating the 94 GHz electron spin resonance of admixed TEMPO nitroxide radicals in a magnetic field of 3.35 T during ca. 3 h at 1.2 K. The sample was subsequently transferred to a high-resolution magnet, and the enhanced polarization was converted from (15)N to methyl- and methylene protons, using the small (2,3)J((1)H,(15)N) couplings in choline. The room-temperature lifetime of nitrogen polarization in choline, T(1)((15)N) approximately 200 s, could be considerably increased by partial deuteration of the molecule. This procedure enables studies of choline metabolites in vitro and in vivo using DNP-enhanced proton NMR. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
volume
131
issue
44
pages
16014 - 16014
publisher
The American Chemical Society
external identifiers
  • wos:000271513700025
  • pmid:19848401
  • scopus:70450202944
ISSN
1520-5126
DOI
10.1021/ja9021304
language
English
LU publication?
yes
id
3877ed83-bef5-4b26-a9c5-29c2b10dcf4a (old id 1500087)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19848401?dopt=Abstract
date added to LUP
2009-11-03 13:08:40
date last changed
2017-10-01 04:18:34
@article{3877ed83-bef5-4b26-a9c5-29c2b10dcf4a,
  abstract     = {Chemical shifts of protons can report on metabolic transformations such as the conversion of choline to phosphocholine. To follow such processes in vivo, magnetization can be enhanced by dynamic nuclear polarization (DNP). We have hyperpolarized in this manner nitrogen-15 spins in (15)N-labeled choline up to 3.3% by irradiating the 94 GHz electron spin resonance of admixed TEMPO nitroxide radicals in a magnetic field of 3.35 T during ca. 3 h at 1.2 K. The sample was subsequently transferred to a high-resolution magnet, and the enhanced polarization was converted from (15)N to methyl- and methylene protons, using the small (2,3)J((1)H,(15)N) couplings in choline. The room-temperature lifetime of nitrogen polarization in choline, T(1)((15)N) approximately 200 s, could be considerably increased by partial deuteration of the molecule. This procedure enables studies of choline metabolites in vitro and in vivo using DNP-enhanced proton NMR.},
  author       = {Sarkar, Riddhiman and Comment, Arnaud and Vasos, Paul R and Jannin, Sami and Gruetter, Rolf and Bodenhausen, Geoffrey and Hall, Hélène and Kirik, Deniz and Denisov, Vladimir},
  issn         = {1520-5126},
  language     = {eng},
  number       = {44},
  pages        = {16014--16014},
  publisher    = {The American Chemical Society},
  series       = {Journal of the American Chemical Society},
  title        = {Proton NMR of (15)N-Choline Metabolites Enhanced by Dynamic Nuclear Polarization.},
  url          = {http://dx.doi.org/10.1021/ja9021304},
  volume       = {131},
  year         = {2009},
}