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Organization of the human embryonic ventral mesencephalon.

Nelander Wahlestedt, Jenny LU ; Hebsgaard, Josephine LU and Parmar, Malin LU (2009) In Gene expression patterns : GEP 9. p.555-561
Abstract
The neurons in the ventral mesencephalon (VM) are organized into several nuclei consisting of distinct neuronal populations. These include the dopaminergic (DA) neurons of the substania nigra and ventral tegmental area, the oculomotor (OM) neurons that innervate the muscles controlling eye movement, and the reticular neurons of the red nucleus (RN) involved in motor control and coordination reviewed in Puelles (2007). The factors and genes that control the differentiation of the various neuronal populations in the VM have been extensively studied in the mouse and other model organisms but little is known about the progenitors and their protein expression in the developing human brain. In this study we analyze if key regulators identified... (More)
The neurons in the ventral mesencephalon (VM) are organized into several nuclei consisting of distinct neuronal populations. These include the dopaminergic (DA) neurons of the substania nigra and ventral tegmental area, the oculomotor (OM) neurons that innervate the muscles controlling eye movement, and the reticular neurons of the red nucleus (RN) involved in motor control and coordination reviewed in Puelles (2007). The factors and genes that control the differentiation of the various neuronal populations in the VM have been extensively studied in the mouse and other model organisms but little is known about the progenitors and their protein expression in the developing human brain. In this study we analyze if key regulators identified in rodents are also expressed in the human VM during embryonic development. We report that BLBP and LMX1A mark the floor plate and that FOXA2 is expressed in both the floor plate and basal plate of the human VM. The proneural transcription factors NGN2 and MASH1 are expressed in the ventricular zone of the human VM within and lateral to the floor plate. The post-mitotic DA neurons express TH as well as NURR1 and PITX3. ISL1 and BRN3A can be used to detect the cells of OM and RN, respectively. We show that many key developmental control factors are expressed in a temporal and spatial manner in the human VM essentially corresponding to what has been observed in the mouse. This data therefore suggest similar roles for these factors also in human VM development and dopamine neurogenesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Gene expression patterns : GEP
volume
9
pages
555 - 561
external identifiers
  • WOS:000272435700001
  • PMID:19825428
  • Scopus:70350711981
ISSN
1872-7298
DOI
10.1016/j.gep.2009.10.002
language
English
LU publication?
yes
id
f7deefcf-6e52-44e4-869c-3ff539710092 (old id 1500388)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19825428?dopt=Abstract
date added to LUP
2009-11-03 15:01:48
date last changed
2017-01-01 07:51:36
@article{f7deefcf-6e52-44e4-869c-3ff539710092,
  abstract     = {The neurons in the ventral mesencephalon (VM) are organized into several nuclei consisting of distinct neuronal populations. These include the dopaminergic (DA) neurons of the substania nigra and ventral tegmental area, the oculomotor (OM) neurons that innervate the muscles controlling eye movement, and the reticular neurons of the red nucleus (RN) involved in motor control and coordination reviewed in Puelles (2007). The factors and genes that control the differentiation of the various neuronal populations in the VM have been extensively studied in the mouse and other model organisms but little is known about the progenitors and their protein expression in the developing human brain. In this study we analyze if key regulators identified in rodents are also expressed in the human VM during embryonic development. We report that BLBP and LMX1A mark the floor plate and that FOXA2 is expressed in both the floor plate and basal plate of the human VM. The proneural transcription factors NGN2 and MASH1 are expressed in the ventricular zone of the human VM within and lateral to the floor plate. The post-mitotic DA neurons express TH as well as NURR1 and PITX3. ISL1 and BRN3A can be used to detect the cells of OM and RN, respectively. We show that many key developmental control factors are expressed in a temporal and spatial manner in the human VM essentially corresponding to what has been observed in the mouse. This data therefore suggest similar roles for these factors also in human VM development and dopamine neurogenesis.},
  author       = {Nelander Wahlestedt, Jenny and Hebsgaard, Josephine and Parmar, Malin},
  issn         = {1872-7298},
  language     = {eng},
  pages        = {555--561},
  series       = {Gene expression patterns : GEP},
  title        = {Organization of the human embryonic ventral mesencephalon.},
  url          = {http://dx.doi.org/10.1016/j.gep.2009.10.002},
  volume       = {9},
  year         = {2009},
}