Contribution of {alpha}6-integrins to hematopoietic stem and progenitor cell homing to bone marrow and collaboration with {alpha}4-integrins.
(2006) In Blood 107(Jan 26). p.3503-3510- Abstract
- The laminin receptor integrin alpha 6 chain is ubiquitously expressed in human and mouse hematopoietic stem and progenitor cells. We have studied its role for homing of stem and progenitor cells to mouse hematopoietic tissues In vivo. A function-blocking anti-integrin alpha 6 antibody significantly reduced progenitor cell homing to bone marrow (BM) of lethally irradiated mice, with a corresponding retention of progenitors in blood. Remarkably, the anti-integrin alpha 6 antibody profoundly inhibited BM homing of long-term multilineage engrafting stem cells, studied by competitive repopulation assay and analysis of donor-derived lymphocytes and myeloid cells in blood 16 weeks after transplantation. A similar profound inhibition of long-term... (More)
- The laminin receptor integrin alpha 6 chain is ubiquitously expressed in human and mouse hematopoietic stem and progenitor cells. We have studied its role for homing of stem and progenitor cells to mouse hematopoietic tissues In vivo. A function-blocking anti-integrin alpha 6 antibody significantly reduced progenitor cell homing to bone marrow (BM) of lethally irradiated mice, with a corresponding retention of progenitors in blood. Remarkably, the anti-integrin alpha 6 antibody profoundly inhibited BM homing of long-term multilineage engrafting stem cells, studied by competitive repopulation assay and analysis of donor-derived lymphocytes and myeloid cells in blood 16 weeks after transplantation. A similar profound inhibition of long-term stem cell homing was obtained by using a function-blocking antibody against alpha 4 integrin, studied in parallel. Furthermore, the anti-integrin alpha 6 and alpha 4 antibodies synergistically inhibited homing of short-term repopulating stem cells. Intravenous injection of anti-integrin alpha 6 antibodies, in contrast to antibodies against alpha 4 integrin, did not mobilize progenitors or enhance cytokine-induced mobilization by G-CSF. Our results provide the first evidence for a distinct functional role of integrin alpha 6 receptor during hematopoietic stem and progenitor cell homing and collaboration of alpha 6 integrin with alpha 4 integrin receptors during homing of short-term stem cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/150103
- author
- Qian, Hong LU ; Tryggvason, Karl ; Jacobsen, Sten Eirik W LU and Ekblom, Marja LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 107
- issue
- Jan 26
- pages
- 3503 - 3510
- publisher
- American Society of Hematology
- external identifiers
-
- wos:000237217600029
- pmid:16439681
- scopus:33646392969
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2005-10-3932
- language
- English
- LU publication?
- yes
- id
- ab92f889-7da9-4749-b57f-3b9d9fdfaa9e (old id 150103)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16439681&dopt=Abstract
- date added to LUP
- 2016-04-01 11:43:48
- date last changed
- 2022-06-09 00:28:14
@article{ab92f889-7da9-4749-b57f-3b9d9fdfaa9e,
abstract = {{The laminin receptor integrin alpha 6 chain is ubiquitously expressed in human and mouse hematopoietic stem and progenitor cells. We have studied its role for homing of stem and progenitor cells to mouse hematopoietic tissues In vivo. A function-blocking anti-integrin alpha 6 antibody significantly reduced progenitor cell homing to bone marrow (BM) of lethally irradiated mice, with a corresponding retention of progenitors in blood. Remarkably, the anti-integrin alpha 6 antibody profoundly inhibited BM homing of long-term multilineage engrafting stem cells, studied by competitive repopulation assay and analysis of donor-derived lymphocytes and myeloid cells in blood 16 weeks after transplantation. A similar profound inhibition of long-term stem cell homing was obtained by using a function-blocking antibody against alpha 4 integrin, studied in parallel. Furthermore, the anti-integrin alpha 6 and alpha 4 antibodies synergistically inhibited homing of short-term repopulating stem cells. Intravenous injection of anti-integrin alpha 6 antibodies, in contrast to antibodies against alpha 4 integrin, did not mobilize progenitors or enhance cytokine-induced mobilization by G-CSF. Our results provide the first evidence for a distinct functional role of integrin alpha 6 receptor during hematopoietic stem and progenitor cell homing and collaboration of alpha 6 integrin with alpha 4 integrin receptors during homing of short-term stem cells.}},
author = {{Qian, Hong and Tryggvason, Karl and Jacobsen, Sten Eirik W and Ekblom, Marja}},
issn = {{1528-0020}},
language = {{eng}},
number = {{Jan 26}},
pages = {{3503--3510}},
publisher = {{American Society of Hematology}},
series = {{Blood}},
title = {{Contribution of {alpha}6-integrins to hematopoietic stem and progenitor cell homing to bone marrow and collaboration with {alpha}4-integrins.}},
url = {{http://dx.doi.org/10.1182/blood-2005-10-3932}},
doi = {{10.1182/blood-2005-10-3932}},
volume = {{107}},
year = {{2006}},
}