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The influence of wavelength and probe configuration on findings of a skin vasoconstriction test when using laser Doppler perfusion devices.

Freccero, Carolin LU ; Wollmer, Per LU ; Sundkvist, Göran LU and Svensson, Henry LU (2006) In Microvascular Research 71(Jan 3). p.64-67
Abstract
The aim of this study was to establish the degree to which a standardized test based on laser Doppler blood flow measurement is dependent on the particular equipment set-up being used. For this purpose, we examined finger skin blood flow with laser Doppler instruments in 20 healthy subjects. In laser Doppler perfusion monitoring (LDPM), we used a custom-made probe with two detecting fibers placed 0.25 and 1.2 min from the illuminating fiber, respectively, and two laser Doppler perfusion imagers (LDPI) with a wavelength of 632.8 nm and 780 rim, respectively. Warming of the hand was achieved with a Peltier element, and reflex vasoconstriction was induced by immersing the other hand for 3 min into a water bath kept at 15 degrees C. As a... (More)
The aim of this study was to establish the degree to which a standardized test based on laser Doppler blood flow measurement is dependent on the particular equipment set-up being used. For this purpose, we examined finger skin blood flow with laser Doppler instruments in 20 healthy subjects. In laser Doppler perfusion monitoring (LDPM), we used a custom-made probe with two detecting fibers placed 0.25 and 1.2 min from the illuminating fiber, respectively, and two laser Doppler perfusion imagers (LDPI) with a wavelength of 632.8 nm and 780 rim, respectively. Warming of the hand was achieved with a Peltier element, and reflex vasoconstriction was induced by immersing the other hand for 3 min into a water bath kept at 15 degrees C. As a measure for the change in skin blood flow, a vasoconstriction index (VAC: cooling/before cooling) was calculated and used for the comparison of the different devices. VAC values gathered around 0.6 for all devices. However, LDPI with a wavelength of 632.9 nm showed a slightly higher VAC index, and the difference was significant. We conclude that using a standardized test is the most appropriate for monitoring changes in blood flow rather than recording and comparing discrete values in intermittent recordings. Although a difference was noted when comparing the devices, different fiber separations and wavelengths seem then to be of little consequence. (c) 2005 Published by Elsevier Inc. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
vasoconstriction, skin blood flow, probe configuration, waveleng-th, laser Doppler perfusion monitoring, laser Doppler perfusion imaging, sympathetic
in
Microvascular Research
volume
71
issue
Jan 3
pages
64 - 67
publisher
Academic Press
external identifiers
  • wos:000235435800008
  • pmid:16403539
  • scopus:31744446037
  • pmid:16403539
ISSN
1095-9319
DOI
10.1016/j.mvr.2005.11.002
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Clinical Physiology (Lund) (013013000), Endocrinology (013241500), Reconstructive Surgery (013240300), Surgery Research Unit (013242220), Clinical Physiology and Nuclear Medicine Unit (013242320)
id
e54c9091-4573-4474-8008-6ee07c19eb88 (old id 150436)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16403539&dopt=Abstract
date added to LUP
2016-04-01 11:54:08
date last changed
2021-02-17 04:37:41
@article{e54c9091-4573-4474-8008-6ee07c19eb88,
  abstract     = {The aim of this study was to establish the degree to which a standardized test based on laser Doppler blood flow measurement is dependent on the particular equipment set-up being used. For this purpose, we examined finger skin blood flow with laser Doppler instruments in 20 healthy subjects. In laser Doppler perfusion monitoring (LDPM), we used a custom-made probe with two detecting fibers placed 0.25 and 1.2 min from the illuminating fiber, respectively, and two laser Doppler perfusion imagers (LDPI) with a wavelength of 632.8 nm and 780 rim, respectively. Warming of the hand was achieved with a Peltier element, and reflex vasoconstriction was induced by immersing the other hand for 3 min into a water bath kept at 15 degrees C. As a measure for the change in skin blood flow, a vasoconstriction index (VAC: cooling/before cooling) was calculated and used for the comparison of the different devices. VAC values gathered around 0.6 for all devices. However, LDPI with a wavelength of 632.9 nm showed a slightly higher VAC index, and the difference was significant. We conclude that using a standardized test is the most appropriate for monitoring changes in blood flow rather than recording and comparing discrete values in intermittent recordings. Although a difference was noted when comparing the devices, different fiber separations and wavelengths seem then to be of little consequence. (c) 2005 Published by Elsevier Inc.},
  author       = {Freccero, Carolin and Wollmer, Per and Sundkvist, Göran and Svensson, Henry},
  issn         = {1095-9319},
  language     = {eng},
  number       = {Jan 3},
  pages        = {64--67},
  publisher    = {Academic Press},
  series       = {Microvascular Research},
  title        = {The influence of wavelength and probe configuration on findings of a skin vasoconstriction test when using laser Doppler perfusion devices.},
  url          = {http://dx.doi.org/10.1016/j.mvr.2005.11.002},
  doi          = {10.1016/j.mvr.2005.11.002},
  volume       = {71},
  year         = {2006},
}