Multilayered Magnetic Nanoparticles as a Support in Solid-Phase Peptide Synthesis
(2009) In International Journal of Peptide Research and Therapeutics 15(4). p.287-292- Abstract
- The synthesis of multilayered magnetic nanoparticles (MNPs) for use as a support in solid-phase peptide synthesis (SPPS) is described. Silanization of magnetite (Fe3O4) nanoparticles with 3-(trimethoxysilyl)propyl methacrylate introduced polymerizable groups on the surface. Polymerization with allylamine, trimethylolpropane trimethacrylate, and trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate provided a polymeric coating and amino groups to serve as starting points for the synthesis. After coupling of an internal reference amino acid and a cleavable linker, the coated MNPs were applied as the solid phase during synthesis of Leu-enkephalinamide and acyl carrier protein (65-74) by Fmoc chemistry. A "high-load" version of the MNP... (More)
- The synthesis of multilayered magnetic nanoparticles (MNPs) for use as a support in solid-phase peptide synthesis (SPPS) is described. Silanization of magnetite (Fe3O4) nanoparticles with 3-(trimethoxysilyl)propyl methacrylate introduced polymerizable groups on the surface. Polymerization with allylamine, trimethylolpropane trimethacrylate, and trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate provided a polymeric coating and amino groups to serve as starting points for the synthesis. After coupling of an internal reference amino acid and a cleavable linker, the coated MNPs were applied as the solid phase during synthesis of Leu-enkephalinamide and acyl carrier protein (65-74) by Fmoc chemistry. A "high-load" version of the MNP support (0.32 mmol/g) was prepared by four consecutive cycles of Fmoc-Lys(Fmoc)-OH coupling and Fmoc deprotection. Successful synthesis of Leu-enkephalin was demonstrated on the "high-load" MNPs. Chemical stability studies proved the particles to be stable under SPPS conditions and magnetization measurements showed that the magnetic properties of the particles were maintained throughout derivatizations and SPPS. The MNPs were further characterized by high-resolution transmission electron microscopy, inductively coupled plasma atomic emission spectrometry, elemental analysis, and nitrogen gas adsorption measurements. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1505081
- author
- Norén, Katarina LU and Kempe, Maria LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Support, Solid-phase peptide synthesis, Polymer, Multilayer structure, Magnetite, Magnetic materials, Nanocomposites
- in
- International Journal of Peptide Research and Therapeutics
- volume
- 15
- issue
- 4
- pages
- 287 - 292
- publisher
- Springer
- external identifiers
-
- wos:000271263800006
- scopus:70350786917
- ISSN
- 1573-3904
- DOI
- 10.1007/s10989-009-9190-3
- language
- English
- LU publication?
- yes
- id
- efaa52cb-fe37-457f-9039-81f63426907c (old id 1505081)
- date added to LUP
- 2016-04-01 12:03:35
- date last changed
- 2022-01-26 22:12:56
@article{efaa52cb-fe37-457f-9039-81f63426907c, abstract = {{The synthesis of multilayered magnetic nanoparticles (MNPs) for use as a support in solid-phase peptide synthesis (SPPS) is described. Silanization of magnetite (Fe3O4) nanoparticles with 3-(trimethoxysilyl)propyl methacrylate introduced polymerizable groups on the surface. Polymerization with allylamine, trimethylolpropane trimethacrylate, and trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate provided a polymeric coating and amino groups to serve as starting points for the synthesis. After coupling of an internal reference amino acid and a cleavable linker, the coated MNPs were applied as the solid phase during synthesis of Leu-enkephalinamide and acyl carrier protein (65-74) by Fmoc chemistry. A "high-load" version of the MNP support (0.32 mmol/g) was prepared by four consecutive cycles of Fmoc-Lys(Fmoc)-OH coupling and Fmoc deprotection. Successful synthesis of Leu-enkephalin was demonstrated on the "high-load" MNPs. Chemical stability studies proved the particles to be stable under SPPS conditions and magnetization measurements showed that the magnetic properties of the particles were maintained throughout derivatizations and SPPS. The MNPs were further characterized by high-resolution transmission electron microscopy, inductively coupled plasma atomic emission spectrometry, elemental analysis, and nitrogen gas adsorption measurements.}}, author = {{Norén, Katarina and Kempe, Maria}}, issn = {{1573-3904}}, keywords = {{Support; Solid-phase peptide synthesis; Polymer; Multilayer structure; Magnetite; Magnetic materials; Nanocomposites}}, language = {{eng}}, number = {{4}}, pages = {{287--292}}, publisher = {{Springer}}, series = {{International Journal of Peptide Research and Therapeutics}}, title = {{Multilayered Magnetic Nanoparticles as a Support in Solid-Phase Peptide Synthesis}}, url = {{http://dx.doi.org/10.1007/s10989-009-9190-3}}, doi = {{10.1007/s10989-009-9190-3}}, volume = {{15}}, year = {{2009}}, }