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4.1R-deficient human red blood cells have altered phosphatidylserine exposure pathways and are deficient in CD44 and CD47 glycoproteins

Jeremy, Kris P.; Plummer, Zoe E.; Head, David J.; Madgett, Tracey E.; Sanders, Kelly L.; Wallington, Amanda; Storry, Jill LU ; Gilsanz, Florinda; Delaunay, Jean and Avent, Neil D. (2009) In Haematologica 94(10). p.1354-1361
Abstract
Background Protein 4.1R is an important component of die red cell membrane skeleton. It imparts structural integrity and has transmembrane signaling roles by direct interactions with transmembrane proteins and other membrane skeletal components, notably p55 and calmodulin. Design and Methods Spontaneous and ligation-induced phosphatidylserine exposure on erythrocytes from two patients with 4.1R deficiency were studied, using CD47 glycoprotein and glycophorin C as ligands We also looked for protein abnormalities in the 4.1R - based multiprotein complex. Results Phosphatidylserine exposure was significantly increased in 4.1R-deficient erythrocytes obtained from the two different individuals when ligands to CD47 glycoprotein were bound.... (More)
Background Protein 4.1R is an important component of die red cell membrane skeleton. It imparts structural integrity and has transmembrane signaling roles by direct interactions with transmembrane proteins and other membrane skeletal components, notably p55 and calmodulin. Design and Methods Spontaneous and ligation-induced phosphatidylserine exposure on erythrocytes from two patients with 4.1R deficiency were studied, using CD47 glycoprotein and glycophorin C as ligands We also looked for protein abnormalities in the 4.1R - based multiprotein complex. Results Phosphatidylserine exposure was significantly increased in 4.1R-deficient erythrocytes obtained from the two different individuals when ligands to CD47 glycoprotein were bound. Spontaneous phosphatidylserine exposure was normal. 4.1R, glycophorin C and p55 were missing or sharply reduced. Furthermore there was an alteration or deficiency of CD47 glycoprotein and a lack of CD44 glycoprotein. Based on a recent study in 4.1R-deficient mice, we found that there are clear functional differences between interactions of human red cell 4.1R and its murine counterpart Conclusions Glycophorin C is known to bind 4.1R, and we have defined previously that it also binds CD47 From our evidence, we suggest that 4.1R plays a role in the phosphatidylserine exposure signaling pathway that is of Fundamental importance in red cell turnover The linkage of CD44 to 4.1R may be relevant to this process (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glycophorin C, CD47, CD44, phosphatidylserine, 4.1R deficiency
in
Haematologica
volume
94
issue
10
pages
1354 - 1361
publisher
Ferrata Storti Foundation
external identifiers
  • wos:000271092500006
  • scopus:70349638633
ISSN
1592-8721
DOI
10.3324/haematol.2009.006585
language
English
LU publication?
yes
id
dffa900c-7cd9-4bd6-98ed-8985fab25f53 (old id 1505537)
date added to LUP
2009-11-24 11:11:06
date last changed
2017-01-01 05:26:48
@article{dffa900c-7cd9-4bd6-98ed-8985fab25f53,
  abstract     = {Background Protein 4.1R is an important component of die red cell membrane skeleton. It imparts structural integrity and has transmembrane signaling roles by direct interactions with transmembrane proteins and other membrane skeletal components, notably p55 and calmodulin. Design and Methods Spontaneous and ligation-induced phosphatidylserine exposure on erythrocytes from two patients with 4.1R deficiency were studied, using CD47 glycoprotein and glycophorin C as ligands We also looked for protein abnormalities in the 4.1R - based multiprotein complex. Results Phosphatidylserine exposure was significantly increased in 4.1R-deficient erythrocytes obtained from the two different individuals when ligands to CD47 glycoprotein were bound. Spontaneous phosphatidylserine exposure was normal. 4.1R, glycophorin C and p55 were missing or sharply reduced. Furthermore there was an alteration or deficiency of CD47 glycoprotein and a lack of CD44 glycoprotein. Based on a recent study in 4.1R-deficient mice, we found that there are clear functional differences between interactions of human red cell 4.1R and its murine counterpart Conclusions Glycophorin C is known to bind 4.1R, and we have defined previously that it also binds CD47 From our evidence, we suggest that 4.1R plays a role in the phosphatidylserine exposure signaling pathway that is of Fundamental importance in red cell turnover The linkage of CD44 to 4.1R may be relevant to this process},
  author       = {Jeremy, Kris P. and Plummer, Zoe E. and Head, David J. and Madgett, Tracey E. and Sanders, Kelly L. and Wallington, Amanda and Storry, Jill and Gilsanz, Florinda and Delaunay, Jean and Avent, Neil D.},
  issn         = {1592-8721},
  keyword      = {glycophorin C,CD47,CD44,phosphatidylserine,4.1R deficiency},
  language     = {eng},
  number       = {10},
  pages        = {1354--1361},
  publisher    = {Ferrata Storti Foundation},
  series       = {Haematologica},
  title        = {4.1R-deficient human red blood cells have altered phosphatidylserine exposure pathways and are deficient in CD44 and CD47 glycoproteins},
  url          = {http://dx.doi.org/10.3324/haematol.2009.006585},
  volume       = {94},
  year         = {2009},
}