Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles.
(2006) In Journal of Leukocyte Biology 79. p.564-573- Abstract
- Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling... (More)
- Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL-8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/150610
- author
- Pellmé, Sara ; Mörgelin, Matthias LU ; Tapper, Hans LU ; Mellqvist, Ulf-Henrik ; Dahlgren, Claes and Karlsson, Anna
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cell activation, subcellular organization, chemokines, inflammation
- in
- Journal of Leukocyte Biology
- volume
- 79
- pages
- 564 - 573
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000243015000016
- pmid:16365151
- scopus:33646110529
- ISSN
- 1938-3673
- DOI
- 10.1189/jlb.0505248
- language
- English
- LU publication?
- yes
- id
- d52eff32-7915-4c13-a9ae-da039428a441 (old id 150610)
- date added to LUP
- 2016-04-01 12:16:33
- date last changed
- 2022-01-27 01:23:00
@article{d52eff32-7915-4c13-a9ae-da039428a441, abstract = {{Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL-8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil.}}, author = {{Pellmé, Sara and Mörgelin, Matthias and Tapper, Hans and Mellqvist, Ulf-Henrik and Dahlgren, Claes and Karlsson, Anna}}, issn = {{1938-3673}}, keywords = {{cell activation; subcellular organization; chemokines; inflammation}}, language = {{eng}}, pages = {{564--573}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Leukocyte Biology}}, title = {{Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles.}}, url = {{http://dx.doi.org/10.1189/jlb.0505248}}, doi = {{10.1189/jlb.0505248}}, volume = {{79}}, year = {{2006}}, }