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Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles.

Pellmé, Sara ; Mörgelin, Matthias LU ; Tapper, Hans LU ; Mellqvist, Ulf-Henrik ; Dahlgren, Claes and Karlsson, Anna (2006) In Journal of Leukocyte Biology 79. p.564-573
Abstract
Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling... (More)
Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL-8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell activation, subcellular organization, chemokines, inflammation
in
Journal of Leukocyte Biology
volume
79
pages
564 - 573
publisher
John Wiley and Sons
external identifiers
  • wos:000243015000016
  • pmid:16365151
  • scopus:33646110529
ISSN
1938-3673
DOI
10.1189/jlb.0505248
language
English
LU publication?
yes
id
d52eff32-7915-4c13-a9ae-da039428a441 (old id 150610)
date added to LUP
2016-04-01 12:16:33
date last changed
2021-10-06 05:27:14
@article{d52eff32-7915-4c13-a9ae-da039428a441,
  abstract     = {Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL-8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil.},
  author       = {Pellmé, Sara and Mörgelin, Matthias and Tapper, Hans and Mellqvist, Ulf-Henrik and Dahlgren, Claes and Karlsson, Anna},
  issn         = {1938-3673},
  language     = {eng},
  pages        = {564--573},
  publisher    = {John Wiley and Sons},
  series       = {Journal of Leukocyte Biology},
  title        = {Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles.},
  url          = {http://dx.doi.org/10.1189/jlb.0505248},
  doi          = {10.1189/jlb.0505248},
  volume       = {79},
  year         = {2006},
}