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Variation among Colletotrichum gloeosporioides isolates from infected coffee berries at different locations in Vietnam

Nguyen, T. H. P.; Säll, Torbjörn LU ; Bryngelsson, T. and Liljeroth, E. (2009) In Plant Pathology 58(5). p.898-909
Abstract
Isolates of Colletotrichum, gloeosporioides associated with anthracnose disease oil coffee berries in Vietnam were characterized by morphological and molecular methods. Random amplified polymorphic DNA (RAPD) and microsatellite-primered PCR (MP-PCR) analyses were employed to investigate the genetic variation among 38 and 51 isolates of C. gloeosporioides, respectively. According to both methods, the isolates mainly grouped in accordance with geographical origins. Higher genetic variation (H = 0.312 and 0.335) in the northern Population of C. gloeosporioides than ill the Southern population (H = 0.261 and 0.186), according to the RAPD and MP-PCR markets, respectively, was indicative of a difference between the northern and southern... (More)
Isolates of Colletotrichum, gloeosporioides associated with anthracnose disease oil coffee berries in Vietnam were characterized by morphological and molecular methods. Random amplified polymorphic DNA (RAPD) and microsatellite-primered PCR (MP-PCR) analyses were employed to investigate the genetic variation among 38 and 51 isolates of C. gloeosporioides, respectively. According to both methods, the isolates mainly grouped in accordance with geographical origins. Higher genetic variation (H = 0.312 and 0.335) in the northern Population of C. gloeosporioides than ill the Southern population (H = 0.261 and 0.186), according to the RAPD and MP-PCR markets, respectively, was indicative of a difference between the northern and southern Populations. Moderate gene differentiation (Gst = 0.1) between Populations from the north and the south was found. However, there was no differentiation between locations within the northern or Southern populations, indicating significant gene flow. A four-gamete test indicated a high level of recombination, particularly Ill the South. The geographic differences may be explained by different histories of coffee cultivation in different parts of Vietnam. The symptoms caused by the Vietnamese isolates on both hypocotyls and green berries were less severe than symptoms caused by the reference CBD (coffee berry disease; Colletotrichum kahalvae) isolates originating from Africa. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
genetic variation, anthracnose disease, Coffea spp., MP-PCR, pathogenicity, RAPD
in
Plant Pathology
volume
58
issue
5
pages
898 - 909
publisher
Wiley-Blackwell
external identifiers
  • wos:000270592900011
  • scopus:70249130179
ISSN
1365-3059
DOI
10.1111/j.1365-3059.2009.02085.x
language
English
LU publication?
yes
id
1d5360d6-cd19-4011-9325-da131e3b7a56 (old id 1507255)
date added to LUP
2009-11-20 15:17:12
date last changed
2017-02-12 03:28:54
@article{1d5360d6-cd19-4011-9325-da131e3b7a56,
  abstract     = {Isolates of Colletotrichum, gloeosporioides associated with anthracnose disease oil coffee berries in Vietnam were characterized by morphological and molecular methods. Random amplified polymorphic DNA (RAPD) and microsatellite-primered PCR (MP-PCR) analyses were employed to investigate the genetic variation among 38 and 51 isolates of C. gloeosporioides, respectively. According to both methods, the isolates mainly grouped in accordance with geographical origins. Higher genetic variation (H = 0.312 and 0.335) in the northern Population of C. gloeosporioides than ill the Southern population (H = 0.261 and 0.186), according to the RAPD and MP-PCR markets, respectively, was indicative of a difference between the northern and southern Populations. Moderate gene differentiation (Gst = 0.1) between Populations from the north and the south was found. However, there was no differentiation between locations within the northern or Southern populations, indicating significant gene flow. A four-gamete test indicated a high level of recombination, particularly Ill the South. The geographic differences may be explained by different histories of coffee cultivation in different parts of Vietnam. The symptoms caused by the Vietnamese isolates on both hypocotyls and green berries were less severe than symptoms caused by the reference CBD (coffee berry disease; Colletotrichum kahalvae) isolates originating from Africa.},
  author       = {Nguyen, T. H. P. and Säll, Torbjörn and Bryngelsson, T. and Liljeroth, E.},
  issn         = {1365-3059},
  keyword      = {genetic variation,anthracnose disease,Coffea spp.,MP-PCR,pathogenicity,RAPD},
  language     = {eng},
  number       = {5},
  pages        = {898--909},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Pathology},
  title        = {Variation among Colletotrichum gloeosporioides isolates from infected coffee berries at different locations in Vietnam},
  url          = {http://dx.doi.org/10.1111/j.1365-3059.2009.02085.x},
  volume       = {58},
  year         = {2009},
}