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Peptides analysis in blood plasma using on-line system of supported liquid membrane and high-performance liquid chromatography

Drapala, Anna; Jönsson, Jan Åke LU and Wieczorek, Piotr (2005) In Analytica Chimica Acta 553(1-2). p.9-14
Abstract
The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the transport of investigated peptides. After extraction, the analyte-enriched acceptor phase was directly injected into an HPLC system for analysis. With SLM, high selectivity and efficiency were achieved for extraction of peptides in aqueous solutions. Lower extraction efficiency was obtained in plasma sample. The limiting factors were the presence of... (More)
The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the transport of investigated peptides. After extraction, the analyte-enriched acceptor phase was directly injected into an HPLC system for analysis. With SLM, high selectivity and efficiency were achieved for extraction of peptides in aqueous solutions. Lower extraction efficiency was obtained in plasma sample. The limiting factors were the presence of salts and proteins in blood plasma. The necessary of ultrafiltration application before the SLM was required in order to improve its stability. The minimum quantifiable concentration was 90-130 ng/ml of the investigated peptides based on 0.5 ml plasma. The standard curve was linear over the concentration range of 0.05-10 mu g/ml. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
553
issue
1-2
pages
9 - 14
publisher
Elsevier
external identifiers
  • wos:000233077800002
  • scopus:27144513746
ISSN
1873-4324
DOI
10.1016/j.aca.2005.08.008
language
English
LU publication?
yes
id
b94279d4-6d46-48e8-8c40-f26e9f46735d (old id 150890)
date added to LUP
2007-06-26 14:36:47
date last changed
2017-08-20 04:30:16
@article{b94279d4-6d46-48e8-8c40-f26e9f46735d,
  abstract     = {The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the transport of investigated peptides. After extraction, the analyte-enriched acceptor phase was directly injected into an HPLC system for analysis. With SLM, high selectivity and efficiency were achieved for extraction of peptides in aqueous solutions. Lower extraction efficiency was obtained in plasma sample. The limiting factors were the presence of salts and proteins in blood plasma. The necessary of ultrafiltration application before the SLM was required in order to improve its stability. The minimum quantifiable concentration was 90-130 ng/ml of the investigated peptides based on 0.5 ml plasma. The standard curve was linear over the concentration range of 0.05-10 mu g/ml. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Drapala, Anna and Jönsson, Jan Åke and Wieczorek, Piotr},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1-2},
  pages        = {9--14},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Peptides analysis in blood plasma using on-line system of supported liquid membrane and high-performance liquid chromatography},
  url          = {http://dx.doi.org/10.1016/j.aca.2005.08.008},
  volume       = {553},
  year         = {2005},
}