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Equilibrium sampling through membrane based on a single hollow fibre for determination of drug-protein binding and free drug concentration in plasma

Trtic, Tatjana LU ; Liu, Jing-fu LU and Jönsson, Jan Åke LU (2005) In Journal of Chromatography. B 826(1-2). p.169-176
Abstract
The determination of drug-protein binding and free drug concentration in plasma applying the equilibrium sampling through membrane (ESTM) technique has been studied using supported liquid membrane extraction in a single hollow fibre without any membrane carrier. In the extraction setup, the donor phase (plasma or buffer) was placed in the vial, into which was immersed the hollow fibre with the acceptor phase situated in the lumen. This proposed technique was applied to study the drug-protein binding of five local anaesthetics and two antidepressants as model substances, and the influence of the total drug concentration on the drug-protein binding was investigated. The brief theoretical background for determination of the drug-protein... (More)
The determination of drug-protein binding and free drug concentration in plasma applying the equilibrium sampling through membrane (ESTM) technique has been studied using supported liquid membrane extraction in a single hollow fibre without any membrane carrier. In the extraction setup, the donor phase (plasma or buffer) was placed in the vial, into which was immersed the hollow fibre with the acceptor phase situated in the lumen. This proposed technique was applied to study the drug-protein binding of five local anaesthetics and two antidepressants as model substances, and the influence of the total drug concentration on the drug-protein binding was investigated. The brief theoretical background for determination of the drug-protein binding under equilibrium conditions is described. The developed method shows a new, improved and simple procedure for determination of free drug concentration in plasma and extent of drug-protein binding. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
local anaesthetic, drug-protein binding, antidepressant, hollow fibre, equilibrium sampling through membrane (ESTM)
in
Journal of Chromatography. B
volume
826
issue
1-2
pages
169 - 176
publisher
Elsevier
external identifiers
  • pmid:16181816
  • wos:000232872700022
  • scopus:26844568469
ISSN
1873-376X
DOI
10.1016/j.jchromb.2005.08.020
language
English
LU publication?
yes
id
d8b84b64-fe81-4a13-8dbc-ba0e6795a429 (old id 150906)
date added to LUP
2007-06-28 09:16:40
date last changed
2017-01-01 04:53:50
@article{d8b84b64-fe81-4a13-8dbc-ba0e6795a429,
  abstract     = {The determination of drug-protein binding and free drug concentration in plasma applying the equilibrium sampling through membrane (ESTM) technique has been studied using supported liquid membrane extraction in a single hollow fibre without any membrane carrier. In the extraction setup, the donor phase (plasma or buffer) was placed in the vial, into which was immersed the hollow fibre with the acceptor phase situated in the lumen. This proposed technique was applied to study the drug-protein binding of five local anaesthetics and two antidepressants as model substances, and the influence of the total drug concentration on the drug-protein binding was investigated. The brief theoretical background for determination of the drug-protein binding under equilibrium conditions is described. The developed method shows a new, improved and simple procedure for determination of free drug concentration in plasma and extent of drug-protein binding. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Trtic, Tatjana and Liu, Jing-fu and Jönsson, Jan Åke},
  issn         = {1873-376X},
  keyword      = {local anaesthetic,drug-protein binding,antidepressant,hollow fibre,equilibrium sampling through membrane (ESTM)},
  language     = {eng},
  number       = {1-2},
  pages        = {169--176},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Equilibrium sampling through membrane based on a single hollow fibre for determination of drug-protein binding and free drug concentration in plasma},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2005.08.020},
  volume       = {826},
  year         = {2005},
}