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Characterization of graphite electrodes modified with laccases from Trametes hirsuta and Cerrena unicolor and their use for flow injection amperometric determination of some phenolic compounds

Haghighi, Behzad LU ; Jarosz-Wilkolazka, A; Ruzgas, Tautgirdas LU ; Gorton, Lo LU and Leonowicz, A (2005) In International Journal of Environmental Analytical Chemistry 85(9-11). p.753-770
Abstract
Fungal laccases from Trametes hirsuta and Cerrena unicolor were immobilized on spectroscopic graphite electrodes using physical absorption. The laccase-modified graphite electrodes were mounted as the working electrodes into a wall jet flow through an electrochemical cell and then used for flow injection amperometric determination of different phenolic compounds of relevance both for environmental and clinical analysis. At optimum conditions for determination of catechol serving as a model compound, the output response signals of the laccase-modified electrodes were recorded as the result of injections of 50 mu L of solutions with different phenolic Substrates into the carrier citrate buffer solution with a working potential of -50mV vs.... (More)
Fungal laccases from Trametes hirsuta and Cerrena unicolor were immobilized on spectroscopic graphite electrodes using physical absorption. The laccase-modified graphite electrodes were mounted as the working electrodes into a wall jet flow through an electrochemical cell and then used for flow injection amperometric determination of different phenolic compounds of relevance both for environmental and clinical analysis. At optimum conditions for determination of catechol serving as a model compound, the output response signals of the laccase-modified electrodes were recorded as the result of injections of 50 mu L of solutions with different phenolic Substrates into the carrier citrate buffer solution with a working potential of -50mV vs. Ag vertical bar AgCl. Statistical aspects were applied and the Michaelis-Menten constants evaluated were correlated to the chemical structure or the investigated phenolic compounds. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phenols, Laccase, Amperometry, Flow injection, Biosensors
in
International Journal of Environmental Analytical Chemistry
volume
85
issue
9-11
pages
753 - 770
publisher
Taylor & Francis
external identifiers
  • wos:000231982500016
  • scopus:33644668847
ISSN
1029-0397
DOI
10.1080/03067310500153710
language
English
LU publication?
yes
id
3efe1ca1-b1c0-4cfb-a200-91cb4963a6aa (old id 150942)
date added to LUP
2007-06-27 08:36:24
date last changed
2017-01-01 04:48:39
@article{3efe1ca1-b1c0-4cfb-a200-91cb4963a6aa,
  abstract     = {Fungal laccases from Trametes hirsuta and Cerrena unicolor were immobilized on spectroscopic graphite electrodes using physical absorption. The laccase-modified graphite electrodes were mounted as the working electrodes into a wall jet flow through an electrochemical cell and then used for flow injection amperometric determination of different phenolic compounds of relevance both for environmental and clinical analysis. At optimum conditions for determination of catechol serving as a model compound, the output response signals of the laccase-modified electrodes were recorded as the result of injections of 50 mu L of solutions with different phenolic Substrates into the carrier citrate buffer solution with a working potential of -50mV vs. Ag vertical bar AgCl. Statistical aspects were applied and the Michaelis-Menten constants evaluated were correlated to the chemical structure or the investigated phenolic compounds.},
  author       = {Haghighi, Behzad and Jarosz-Wilkolazka, A and Ruzgas, Tautgirdas and Gorton, Lo and Leonowicz, A},
  issn         = {1029-0397},
  keyword      = {Phenols,Laccase,Amperometry,Flow injection,Biosensors},
  language     = {eng},
  number       = {9-11},
  pages        = {753--770},
  publisher    = {Taylor & Francis},
  series       = {International Journal of Environmental Analytical Chemistry},
  title        = {Characterization of graphite electrodes modified with laccases from Trametes hirsuta and Cerrena unicolor and their use for flow injection amperometric determination of some phenolic compounds},
  url          = {http://dx.doi.org/10.1080/03067310500153710},
  volume       = {85},
  year         = {2005},
}