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Direct electron transfer reactions of laccases from different origins on carbon electrodes

Shleev, Sergey LU ; Jarosz-Wilkolazka, A; Khalunina, A; Morozova, O; Yaropolov, A; Ruzgas, Tautgirdas LU and Gorton, Lo LU (2005) In Bioelectrochemistry 67(1). p.115-124
Abstract
Electrochemical studies of laccases from basidiomycetes, i.e., Trametes hirsuta, Trametes ochracea, Coriolopsis fulvocinerea, Cerrena maxima, and Cerrena unicolor, have been performed. Direct (mediatorless) electrochemistry of laccases on graphite electrodes has been investigated with cyclic voltammetry, square wave voltammetry as well as potentiometry. For all mentioned high potential laccases direct electron transfer (DET) has been registered at spectrographic graphite and highly ordered pyrolytic graphite electrodes. The characteristics of DET reactions of the enzymes were analysed under aerobic and anaerobic conditions. It is shown that the T I site of the laccase is the primary electron acceptor, both in solution (homogenous case) and... (More)
Electrochemical studies of laccases from basidiomycetes, i.e., Trametes hirsuta, Trametes ochracea, Coriolopsis fulvocinerea, Cerrena maxima, and Cerrena unicolor, have been performed. Direct (mediatorless) electrochemistry of laccases on graphite electrodes has been investigated with cyclic voltammetry, square wave voltammetry as well as potentiometry. For all mentioned high potential laccases direct electron transfer (DET) has been registered at spectrographic graphite and highly ordered pyrolytic graphite electrodes. The characteristics of DET reactions of the enzymes were analysed under aerobic and anaerobic conditions. It is shown that the T I site of the laccase is the primary electron acceptor, both in solution (homogenous case) and at surface of the graphite electrode (heterogeneous case). A mechanism of ET for the process of the electroreduction of oxygen at the laccase-modified graphite electrodes is proposed and the similarity of this heterogeneous process to the laccase catalysed oxygen reduction homogeneous reaction is concluded. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioelectrochemistry
volume
67
issue
1
pages
115 - 124
publisher
Elsevier
external identifiers
  • pmid:15941673
  • wos:000232022000014
  • scopus:20444440241
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2005.02.004
language
English
LU publication?
yes
id
aedbadf3-6132-40f8-80a8-77d2f6aaf6b0 (old id 150949)
date added to LUP
2007-06-27 15:48:31
date last changed
2017-10-01 03:58:28
@article{aedbadf3-6132-40f8-80a8-77d2f6aaf6b0,
  abstract     = {Electrochemical studies of laccases from basidiomycetes, i.e., Trametes hirsuta, Trametes ochracea, Coriolopsis fulvocinerea, Cerrena maxima, and Cerrena unicolor, have been performed. Direct (mediatorless) electrochemistry of laccases on graphite electrodes has been investigated with cyclic voltammetry, square wave voltammetry as well as potentiometry. For all mentioned high potential laccases direct electron transfer (DET) has been registered at spectrographic graphite and highly ordered pyrolytic graphite electrodes. The characteristics of DET reactions of the enzymes were analysed under aerobic and anaerobic conditions. It is shown that the T I site of the laccase is the primary electron acceptor, both in solution (homogenous case) and at surface of the graphite electrode (heterogeneous case). A mechanism of ET for the process of the electroreduction of oxygen at the laccase-modified graphite electrodes is proposed and the similarity of this heterogeneous process to the laccase catalysed oxygen reduction homogeneous reaction is concluded. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Shleev, Sergey and Jarosz-Wilkolazka, A and Khalunina, A and Morozova, O and Yaropolov, A and Ruzgas, Tautgirdas and Gorton, Lo},
  issn         = {1878-562X},
  language     = {eng},
  number       = {1},
  pages        = {115--124},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Direct electron transfer reactions of laccases from different origins on carbon electrodes},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2005.02.004},
  volume       = {67},
  year         = {2005},
}