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Pathway proteomics - Global and focused approaches

Marko-Varga, György LU (2005) In American Journal of PharmacoGenomics 5(2). p.113-122
Abstract
Biological pathways represent the relationships (reactions and interactions) between biological molecules in the context of normal cellular functions and disease mechanisms. Understanding the roles of proteins and signaling pathways expressed within disease, and their link to drug discovery and drug development are central in today's target-driven pharmaceutical processes. This article gives an overview of proteomics strategies, including global expression analysis as well as focused approaches using multidimensional separation by both gel- and liquid-phase techniques linked to mass spectrometry, as applied to two of the pathways involved in inflammatory diseases. In primary human cell studies, our group has annotated and identified... (More)
Biological pathways represent the relationships (reactions and interactions) between biological molecules in the context of normal cellular functions and disease mechanisms. Understanding the roles of proteins and signaling pathways expressed within disease, and their link to drug discovery and drug development are central in today's target-driven pharmaceutical processes. This article gives an overview of proteomics strategies, including global expression analysis as well as focused approaches using multidimensional separation by both gel- and liquid-phase techniques linked to mass spectrometry, as applied to two of the pathways involved in inflammatory diseases. In primary human cell studies, our group has annotated and identified thousands of proteins using both electrospray ionization and matrix-assisted laser desorption ionization (MALDI)-sequencing technology. Annotations made from gel images and chromatography fractionation, interfaced to high-end mass spectrometry sequence and structure identity, are cornerstones in cutting-edge protein expression profiling. Regarding phosphorylation mechanisms of kinases, the quantitative stoichiometry can be determined using affinity probe isolations. Another strategy involves micro-preparative sample processing, which has been used to analyze single-target phosphoproteins and their relative phospho-stoichiometry. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of PharmacoGenomics
volume
5
issue
2
pages
113 - 122
publisher
Adis International
external identifiers
  • pmid:15813674
  • wos:000231695700004
  • scopus:17844393883
ISSN
1175-2203
language
English
LU publication?
yes
id
bbd455b5-644a-4986-ae14-99a91c8ea424 (old id 150971)
alternative location
http://pt.wkhealth.com/pt/re/pcg/home.htm;jsessionid=Dpe497kVDKE2kSY915C011CAwGHjMQ0DPwpeWQjpPgvU1L7PwbQU!-1926323623!-949856144!9001!-1
date added to LUP
2007-06-27 11:10:27
date last changed
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@article{bbd455b5-644a-4986-ae14-99a91c8ea424,
  abstract     = {Biological pathways represent the relationships (reactions and interactions) between biological molecules in the context of normal cellular functions and disease mechanisms. Understanding the roles of proteins and signaling pathways expressed within disease, and their link to drug discovery and drug development are central in today's target-driven pharmaceutical processes. This article gives an overview of proteomics strategies, including global expression analysis as well as focused approaches using multidimensional separation by both gel- and liquid-phase techniques linked to mass spectrometry, as applied to two of the pathways involved in inflammatory diseases. In primary human cell studies, our group has annotated and identified thousands of proteins using both electrospray ionization and matrix-assisted laser desorption ionization (MALDI)-sequencing technology. Annotations made from gel images and chromatography fractionation, interfaced to high-end mass spectrometry sequence and structure identity, are cornerstones in cutting-edge protein expression profiling. Regarding phosphorylation mechanisms of kinases, the quantitative stoichiometry can be determined using affinity probe isolations. Another strategy involves micro-preparative sample processing, which has been used to analyze single-target phosphoproteins and their relative phospho-stoichiometry.},
  author       = {Marko-Varga, György},
  issn         = {1175-2203},
  language     = {eng},
  number       = {2},
  pages        = {113--122},
  publisher    = {Adis International},
  series       = {American Journal of PharmacoGenomics},
  title        = {Pathway proteomics - Global and focused approaches},
  volume       = {5},
  year         = {2005},
}