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Amperometric detection of mono- and diphenols at Cerrena unicolor laccase-modified graphite electrode: correlation between sensitivity and substrate structure

Jarosz-Wilkolazka, A; Ruzgas, Tautgirdas LU and Gorton, Lo LU (2005) In Talanta 66(5). p.1219-1224
Abstract
Graphite electrode modified with laccase from Cerrena unicolor served as a biosensor for detection of 30 phenolic compounds with different structures. Some correlations of the sensor response to the structures of substrates are discussed. This biosensor responded to: (i) nanomolar concentrations of some of the selected phenolic compounds, e.g., 2,6-dimethoxyphenol,coniferyl alcohol, caffeic acid, DOPAC and hydroquinone, (ii) micromolar concentrations, e.g., ferulic acid, syringic acid, dopamine, 3,4-dihydroxybenzoic acid and DL-noradrenaline, and (iii) millimolar concentrations in the case of phenol and 4-hydroxybenzaldehyde. Among the ortho- or para-substituted phenols, the sensitivity of the C unicolor laccase-modified electrode... (More)
Graphite electrode modified with laccase from Cerrena unicolor served as a biosensor for detection of 30 phenolic compounds with different structures. Some correlations of the sensor response to the structures of substrates are discussed. This biosensor responded to: (i) nanomolar concentrations of some of the selected phenolic compounds, e.g., 2,6-dimethoxyphenol,coniferyl alcohol, caffeic acid, DOPAC and hydroquinone, (ii) micromolar concentrations, e.g., ferulic acid, syringic acid, dopamine, 3,4-dihydroxybenzoic acid and DL-noradrenaline, and (iii) millimolar concentrations in the case of phenol and 4-hydroxybenzaldehyde. Among the ortho- or para-substituted phenols, the sensitivity of the C unicolor laccase-modified electrode increased in the following order -H, -CH3, -OH, -OCH3 and -NH3+ but in the case of para-substituted phenols, the K-m(app) values were lower. The sensitivity of the laccase electrode increased with an additional -OH group in para-substituted phenols. In the case of the selected compounds, kinetic data from electrochemical flow injection system were compared with those obtained from experiments in solution. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Talanta
volume
66
issue
5
pages
1219 - 1224
publisher
Elsevier
external identifiers
  • wos:000229996800019
  • scopus:19744365037
ISSN
1873-3573
DOI
10.1016/j.talanta.2005.01.026
language
English
LU publication?
yes
id
9d062905-d45b-4604-ac37-d092690937c7 (old id 151000)
date added to LUP
2007-06-27 09:01:43
date last changed
2017-06-25 04:25:00
@article{9d062905-d45b-4604-ac37-d092690937c7,
  abstract     = {Graphite electrode modified with laccase from Cerrena unicolor served as a biosensor for detection of 30 phenolic compounds with different structures. Some correlations of the sensor response to the structures of substrates are discussed. This biosensor responded to: (i) nanomolar concentrations of some of the selected phenolic compounds, e.g., 2,6-dimethoxyphenol,coniferyl alcohol, caffeic acid, DOPAC and hydroquinone, (ii) micromolar concentrations, e.g., ferulic acid, syringic acid, dopamine, 3,4-dihydroxybenzoic acid and DL-noradrenaline, and (iii) millimolar concentrations in the case of phenol and 4-hydroxybenzaldehyde. Among the ortho- or para-substituted phenols, the sensitivity of the C unicolor laccase-modified electrode increased in the following order -H, -CH3, -OH, -OCH3 and -NH3+ but in the case of para-substituted phenols, the K-m(app) values were lower. The sensitivity of the laccase electrode increased with an additional -OH group in para-substituted phenols. In the case of the selected compounds, kinetic data from electrochemical flow injection system were compared with those obtained from experiments in solution. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Jarosz-Wilkolazka, A and Ruzgas, Tautgirdas and Gorton, Lo},
  issn         = {1873-3573},
  language     = {eng},
  number       = {5},
  pages        = {1219--1224},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Amperometric detection of mono- and diphenols at Cerrena unicolor laccase-modified graphite electrode: correlation between sensitivity and substrate structure},
  url          = {http://dx.doi.org/10.1016/j.talanta.2005.01.026},
  volume       = {66},
  year         = {2005},
}