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Charges drive selection of specific antibodies by phage display.

Persson, Helena LU ; Persson, Jonas LU ; Danielsson, Lena and Ohlin, Mats LU (2010) In Journal of Immunological Methods 353. p.24-30
Abstract
Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection.... (More)
Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection. Implications of and possible routes to minimize this effect are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunological Methods
volume
353
pages
24 - 30
publisher
Elsevier
external identifiers
  • pmid:19961852
  • wos:000275503100004
  • scopus:77649272743
ISSN
1872-7905
DOI
10.1016/j.jim.2009.11.018
language
English
LU publication?
yes
id
1ef07e08-7b04-4a84-8379-d352dad956f7 (old id 1510941)
date added to LUP
2009-11-26 10:12:25
date last changed
2018-05-29 10:18:23
@article{1ef07e08-7b04-4a84-8379-d352dad956f7,
  abstract     = {Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection. Implications of and possible routes to minimize this effect are discussed.},
  author       = {Persson, Helena and Persson, Jonas and Danielsson, Lena and Ohlin, Mats},
  issn         = {1872-7905},
  language     = {eng},
  pages        = {24--30},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Charges drive selection of specific antibodies by phage display.},
  url          = {http://dx.doi.org/10.1016/j.jim.2009.11.018},
  volume       = {353},
  year         = {2010},
}