Charges drive selection of specific antibodies by phage display.
Persson, Helena LU ; Persson, Jonas LU ; Danielsson, Lena and Ohlin, Mats LU
(2010)
In Journal of Immunological Methods
353.
p.24-30
- Abstract
- Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection.... (More)
- Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection. Implications of and possible routes to minimize this effect are discussed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1510941
- author
- Persson, Helena
LU
; Persson, Jonas
LU
; Danielsson, Lena
and Ohlin, Mats
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunological Methods
- volume
- 353
- pages
- 24 - 30
- publisher
- Elsevier
- external identifiers
-
- pmid:19961852
- wos:000275503100004
- scopus:77649272743
- pmid:19961852
- ISSN
- 1872-7905
- DOI
- 10.1016/j.jim.2009.11.018
- language
- English
- LU publication?
- yes
- id
- 1ef07e08-7b04-4a84-8379-d352dad956f7 (old id 1510941)
- date added to LUP
- 2016-04-01 14:26:14
- date last changed
- 2022-01-28 00:40:45
@article{1ef07e08-7b04-4a84-8379-d352dad956f7,
abstract = {{Phage display technology has emerged as a leading approach to select proteins with improved properties for many different types of applications. The selection typically selects not only for improved binding properties but also for other factors such as efficiency of protein production and folding in Escherichia coli, the host in which the proteins and the phage are produced. Furthermore, the selection methodology is likely to influence the character of retrieved variants. We have now defined the extent whereby the charge of the displayed proteins influence the selection process, resulting in an increased average positive charge among selected proteins in comparison to the proteins that are harbored in the library before selection. Implications of and possible routes to minimize this effect are discussed.}},
author = {{Persson, Helena and Persson, Jonas and Danielsson, Lena and Ohlin, Mats}},
issn = {{1872-7905}},
language = {{eng}},
pages = {{24--30}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{Charges drive selection of specific antibodies by phage display.}},
url = {{http://dx.doi.org/10.1016/j.jim.2009.11.018}},
doi = {{10.1016/j.jim.2009.11.018}},
volume = {{353}},
year = {{2010}},
}