Advanced

Determination of drug-protein binding using supported liquid membrane extraction under equilibrium conditions

Trtic, Tatjana LU and Jönsson, Jan Åke LU (2005) In Journal of Chromatography. B 814(2). p.375-384
Abstract
A technique for determination of drug-protein binding based on a membrane extraction technique termed "equilibrium sampling through membrane (ESTM)" is presented. It involves the establishment of an equilibrium between an aqueous buffer and either a blood plasma sample or a matched buffer, both containing the drug. Analysis of the aqueous buffer in the two cases gives the drug-protein binding. The principle bypasses some sources of systematic error found with common techniques for this measurement based on e.g. ultrafiltration, as it senses the equilibrium conditions without disturbing the sample. The technique is applied to some local anesthetic drugs as model substances and two alternative ways for the evaluation are presented. Results... (More)
A technique for determination of drug-protein binding based on a membrane extraction technique termed "equilibrium sampling through membrane (ESTM)" is presented. It involves the establishment of an equilibrium between an aqueous buffer and either a blood plasma sample or a matched buffer, both containing the drug. Analysis of the aqueous buffer in the two cases gives the drug-protein binding. The principle bypasses some sources of systematic error found with common techniques for this measurement based on e.g. ultrafiltration, as it senses the equilibrium conditions without disturbing the sample. The technique is applied to some local anesthetic drugs as model substances and two alternative ways for the evaluation are presented. Results with these evaluation methods are compared with literature values for the drug-protein binding of these compounds. It is found that the drug-protein binding values obtained are lower than literature values, which is attributed to reduced systematic error. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
local anesthetics, supported liquid membrane, drug-protein binding, equilibrium sampling through, equilibrium extraction, membrane (ESTM), extraction
in
Journal of Chromatography. B
volume
814
issue
2
pages
375 - 384
publisher
Elsevier
external identifiers
  • pmid:15639462
  • wos:000226438400023
  • scopus:11844303487
ISSN
1873-376X
DOI
10.1016/j.jchromb.2004.11.001
language
English
LU publication?
yes
id
dee78cc2-a286-4db0-a100-10494a8262fd (old id 151150)
date added to LUP
2007-06-28 09:18:28
date last changed
2017-09-03 03:54:35
@article{dee78cc2-a286-4db0-a100-10494a8262fd,
  abstract     = {A technique for determination of drug-protein binding based on a membrane extraction technique termed "equilibrium sampling through membrane (ESTM)" is presented. It involves the establishment of an equilibrium between an aqueous buffer and either a blood plasma sample or a matched buffer, both containing the drug. Analysis of the aqueous buffer in the two cases gives the drug-protein binding. The principle bypasses some sources of systematic error found with common techniques for this measurement based on e.g. ultrafiltration, as it senses the equilibrium conditions without disturbing the sample. The technique is applied to some local anesthetic drugs as model substances and two alternative ways for the evaluation are presented. Results with these evaluation methods are compared with literature values for the drug-protein binding of these compounds. It is found that the drug-protein binding values obtained are lower than literature values, which is attributed to reduced systematic error. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Trtic, Tatjana and Jönsson, Jan Åke},
  issn         = {1873-376X},
  keyword      = {local anesthetics,supported liquid membrane,drug-protein binding,equilibrium sampling through,equilibrium extraction,membrane (ESTM),extraction},
  language     = {eng},
  number       = {2},
  pages        = {375--384},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Determination of drug-protein binding using supported liquid membrane extraction under equilibrium conditions},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2004.11.001},
  volume       = {814},
  year         = {2005},
}