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Controlled feeding of cellulases improves conversion of xylose in simultaneous saccharification and co-fermentation for bioethanol production.

Olofsson, Kim LU ; Wiman, Magnus LU and Lidén, Gunnar LU (2010) In Journal of Biotechnology 145. p.168-175
Abstract
Simultaneous saccharification and fermentation (SSF) is an interesting option for ethanol production from lignocellulosic materials. To meet desired overall yields during ethanol production from lignocellulosic materials, it is important to use both hexoses and pentoses. This can be achieved by co-fermentation of sugars in SSF, so called SSCF (simultaneous saccharification and co-fermentation), using genetically modified yeast strains. However, high concentration of glucose in the pretreated material makes xylose utilization challenging due to competitive inhibition of sugar transport. The present work demonstrates a new approach for controlling the glucose release rate from the enzymatic hydrolysis by controlling the addition of enzymes... (More)
Simultaneous saccharification and fermentation (SSF) is an interesting option for ethanol production from lignocellulosic materials. To meet desired overall yields during ethanol production from lignocellulosic materials, it is important to use both hexoses and pentoses. This can be achieved by co-fermentation of sugars in SSF, so called SSCF (simultaneous saccharification and co-fermentation), using genetically modified yeast strains. However, high concentration of glucose in the pretreated material makes xylose utilization challenging due to competitive inhibition of sugar transport. The present work demonstrates a new approach for controlling the glucose release rate from the enzymatic hydrolysis by controlling the addition of enzymes in SSCF using spruce as the raw material. Enzyme kinetics and yeast sugar uptake rates for a recombinant xylose utilizing strain of Saccharomyces cerevisiae, TMB3400, were determined in a real hydrolyzate medium. A simplified model for glucose release and uptake was created, to be used as a tool for control of the glucose concentration in a SSCF process. With help of this model, an SSCF process with efficient co-utilization of glucose and xylose was successfully designed. The results showed that the total xylose uptake could be increased from 40% to as much as 80% by controlling the enzyme feed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
145
pages
168 - 175
publisher
Elsevier
external identifiers
  • wos:000274445700010
  • pmid:19900494
  • scopus:72949096777
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2009.11.001
language
English
LU publication?
yes
id
52cc5351-945f-4f53-b0f7-e0a82128768b (old id 1512123)
date added to LUP
2009-12-01 08:21:00
date last changed
2018-05-29 11:21:35
@article{52cc5351-945f-4f53-b0f7-e0a82128768b,
  abstract     = {Simultaneous saccharification and fermentation (SSF) is an interesting option for ethanol production from lignocellulosic materials. To meet desired overall yields during ethanol production from lignocellulosic materials, it is important to use both hexoses and pentoses. This can be achieved by co-fermentation of sugars in SSF, so called SSCF (simultaneous saccharification and co-fermentation), using genetically modified yeast strains. However, high concentration of glucose in the pretreated material makes xylose utilization challenging due to competitive inhibition of sugar transport. The present work demonstrates a new approach for controlling the glucose release rate from the enzymatic hydrolysis by controlling the addition of enzymes in SSCF using spruce as the raw material. Enzyme kinetics and yeast sugar uptake rates for a recombinant xylose utilizing strain of Saccharomyces cerevisiae, TMB3400, were determined in a real hydrolyzate medium. A simplified model for glucose release and uptake was created, to be used as a tool for control of the glucose concentration in a SSCF process. With help of this model, an SSCF process with efficient co-utilization of glucose and xylose was successfully designed. The results showed that the total xylose uptake could be increased from 40% to as much as 80% by controlling the enzyme feed.},
  author       = {Olofsson, Kim and Wiman, Magnus and Lidén, Gunnar},
  issn         = {1873-4863},
  language     = {eng},
  pages        = {168--175},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Controlled feeding of cellulases improves conversion of xylose in simultaneous saccharification and co-fermentation for bioethanol production.},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2009.11.001},
  volume       = {145},
  year         = {2010},
}