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Cyclodextrins in DNA decompaction.

Gonzalez Perez, Alfredo LU ; Carlstedt, Jonas LU ; Dias, Rita LU and Lindman, Björn LU (2010) In Colloids and Surfaces B: Biointerfaces 76(1). p.20-27
Abstract
Individual T4DNA molecules, previously compacted by using a cationic surfactant (cetyltrimethylammonium bromide, CTAB), were successfully decompacted by the addition of an appropriate concentration of either alpha-cyclodextrin or beta-cyclodextrin (alpha-CD and beta-CD, respectively) due to the formation of inclusion complexes with the surfactant. The process was shown to be a non first-order transition from globules to coils. Density and sound velocity measurements as well as steady state fluorescence spectroscopy have confirmed the approximate CD concentration at which the globule-to-coil transition occurs. Phase maps of the DNA-CTA-CD systems were produced and the CTAB concentration range at which decompaction can be achieved was... (More)
Individual T4DNA molecules, previously compacted by using a cationic surfactant (cetyltrimethylammonium bromide, CTAB), were successfully decompacted by the addition of an appropriate concentration of either alpha-cyclodextrin or beta-cyclodextrin (alpha-CD and beta-CD, respectively) due to the formation of inclusion complexes with the surfactant. The process was shown to be a non first-order transition from globules to coils. Density and sound velocity measurements as well as steady state fluorescence spectroscopy have confirmed the approximate CD concentration at which the globule-to-coil transition occurs. Phase maps of the DNA-CTA-CD systems were produced and the CTAB concentration range at which decompaction can be achieved was determined. Evidences for DNA-CD interaction were found, however, its nature and influence on the decompaction process was not yet determined. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CTAB, DNA, Cyclodextrins, Compaction, Decompaction
in
Colloids and Surfaces B: Biointerfaces
volume
76
issue
1
pages
20 - 27
publisher
Elsevier
external identifiers
  • wos:000274767300003
  • pmid:19897344
  • scopus:74149084443
ISSN
1873-4367
DOI
10.1016/j.colsurfb.2009.10.001
language
English
LU publication?
yes
id
fae0ce8c-5422-4558-8aa7-20268928b25c (old id 1512146)
alternative location
http://www.sciencedirect.com/science/article/B6TFS-4XDD042-2/2/a998a34def4a8fa34c0ba419a3fc5a54
date added to LUP
2009-12-01 08:22:57
date last changed
2018-05-29 11:02:33
@article{fae0ce8c-5422-4558-8aa7-20268928b25c,
  abstract     = {Individual T4DNA molecules, previously compacted by using a cationic surfactant (cetyltrimethylammonium bromide, CTAB), were successfully decompacted by the addition of an appropriate concentration of either alpha-cyclodextrin or beta-cyclodextrin (alpha-CD and beta-CD, respectively) due to the formation of inclusion complexes with the surfactant. The process was shown to be a non first-order transition from globules to coils. Density and sound velocity measurements as well as steady state fluorescence spectroscopy have confirmed the approximate CD concentration at which the globule-to-coil transition occurs. Phase maps of the DNA-CTA-CD systems were produced and the CTAB concentration range at which decompaction can be achieved was determined. Evidences for DNA-CD interaction were found, however, its nature and influence on the decompaction process was not yet determined.},
  author       = {Gonzalez Perez, Alfredo and Carlstedt, Jonas and Dias, Rita and Lindman, Björn},
  issn         = {1873-4367},
  keyword      = {CTAB,DNA,Cyclodextrins,Compaction,Decompaction},
  language     = {eng},
  number       = {1},
  pages        = {20--27},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Cyclodextrins in DNA decompaction.},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2009.10.001},
  volume       = {76},
  year         = {2010},
}