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Aspects of Modeling a preparative ion-exchange step for antibody purification

Nilsson, Bernt LU (2005) In Chemical Engineering & Technology 28(11). p.1367-1374
Abstract
In protein chromatography large molecules have to diffuse into the stationary phase. The molecular sizes of the proteins make the diffusion rates low or very low and the separation can be assumed to be mass transfer limited. In this study three different prepacked columns, with different bead sizes, are used for the separation of IgG and BSA to confirm or reject this assumption in an ion-exchange application. The separations are modeled and calibrated against gradient elution data. Model calibration of reaction-dispersive, transport-dispersive and general rate model structures are discussed and the analysis shows that the columns are best modeled by different model structures, because different behaviors are rate-limiting. Experiments call... (More)
In protein chromatography large molecules have to diffuse into the stationary phase. The molecular sizes of the proteins make the diffusion rates low or very low and the separation can be assumed to be mass transfer limited. In this study three different prepacked columns, with different bead sizes, are used for the separation of IgG and BSA to confirm or reject this assumption in an ion-exchange application. The separations are modeled and calibrated against gradient elution data. Model calibration of reaction-dispersive, transport-dispersive and general rate model structures are discussed and the analysis shows that the columns are best modeled by different model structures, because different behaviors are rate-limiting. Experiments call be described surprisingly well by reaction-dispersive model structures, especially for BSA. Broad elution peaks for IgG indicate mass transfer limitations. Calibration of models capturing diffusive effects shows that the pore diffusion coefficients are much higher than expected, compared with the literature, indicating that there are other forces dominating the mass transport in the beads. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemical Engineering & Technology
volume
28
issue
11
pages
1367 - 1374
publisher
John Wiley & Sons
external identifiers
  • wos:000233468900014
  • scopus:28144451616
ISSN
1521-4125
DOI
10.1002/ceat.200500180
language
English
LU publication?
yes
id
b77981f9-1044-4dc1-b1a4-870484f3d957 (old id 151590)
date added to LUP
2007-06-25 13:45:38
date last changed
2017-01-01 04:42:29
@article{b77981f9-1044-4dc1-b1a4-870484f3d957,
  abstract     = {In protein chromatography large molecules have to diffuse into the stationary phase. The molecular sizes of the proteins make the diffusion rates low or very low and the separation can be assumed to be mass transfer limited. In this study three different prepacked columns, with different bead sizes, are used for the separation of IgG and BSA to confirm or reject this assumption in an ion-exchange application. The separations are modeled and calibrated against gradient elution data. Model calibration of reaction-dispersive, transport-dispersive and general rate model structures are discussed and the analysis shows that the columns are best modeled by different model structures, because different behaviors are rate-limiting. Experiments call be described surprisingly well by reaction-dispersive model structures, especially for BSA. Broad elution peaks for IgG indicate mass transfer limitations. Calibration of models capturing diffusive effects shows that the pore diffusion coefficients are much higher than expected, compared with the literature, indicating that there are other forces dominating the mass transport in the beads.},
  author       = {Nilsson, Bernt},
  issn         = {1521-4125},
  language     = {eng},
  number       = {11},
  pages        = {1367--1374},
  publisher    = {John Wiley & Sons},
  series       = {Chemical Engineering & Technology},
  title        = {Aspects of Modeling a preparative ion-exchange step for antibody purification},
  url          = {http://dx.doi.org/10.1002/ceat.200500180},
  volume       = {28},
  year         = {2005},
}