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Enzymatic synthesis of dipeptide units of the D-D-configuration in aqueous media

Kempe, Maria LU ; Ekberg, Björn and Mosbach, Klaus LU (1990) In International Journal of Peptide and Protein Research 35(2). p.147-152
Abstract
The enzymatic synthesis of dipeptide units of the d-d-configuration in aqueous media, catalysed by muramoyl-pentapeptide carboxypeptidase (E.C.3.4.17.8), is described. Ac-l-Lys(Ac)-d-Ala-d-Lac-OH and Ac-d-Ala-OMe were used as acyl-components. Neutral, basic, and hydrophobic amino acids acting as nucleophiles were incorporated. The enzyme is stereospecific in that only the d-enantiomers of amino acids or amino acid derivatives were incorporated. As nucleophiles, the unmodified amino acids resulted in higher product yields compared with using the corresponding amino acid derivatives. Product yields ranged from 40 to 87%
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Peptide and Protein Research
volume
35
issue
2
pages
147 - 152
publisher
Munksgaard International Publishers
external identifiers
  • scopus:0025209791
ISSN
0367-8377
DOI
10.1111/j.1399-3011.1990.tb00250.x
language
English
LU publication?
yes
id
e749979e-d033-467f-92cc-5ffa37e65ad9 (old id 1516048)
date added to LUP
2009-12-17 08:25:15
date last changed
2017-08-27 05:36:27
@article{e749979e-d033-467f-92cc-5ffa37e65ad9,
  abstract     = {The enzymatic synthesis of dipeptide units of the d-d-configuration in aqueous media, catalysed by muramoyl-pentapeptide carboxypeptidase (E.C.3.4.17.8), is described. Ac-l-Lys(Ac)-d-Ala-d-Lac-OH and Ac-d-Ala-OMe were used as acyl-components. Neutral, basic, and hydrophobic amino acids acting as nucleophiles were incorporated. The enzyme is stereospecific in that only the d-enantiomers of amino acids or amino acid derivatives were incorporated. As nucleophiles, the unmodified amino acids resulted in higher product yields compared with using the corresponding amino acid derivatives. Product yields ranged from 40 to 87%},
  author       = {Kempe, Maria and Ekberg, Björn and Mosbach, Klaus},
  issn         = {0367-8377},
  language     = {eng},
  number       = {2},
  pages        = {147--152},
  publisher    = {Munksgaard International Publishers},
  series       = {International Journal of Peptide and Protein Research},
  title        = {Enzymatic synthesis of dipeptide units of the D-D-configuration in aqueous media},
  url          = {http://dx.doi.org/10.1111/j.1399-3011.1990.tb00250.x},
  volume       = {35},
  year         = {1990},
}