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Binding studies on substrate- and enantio-selective molecularly imprinted polymers

Kempe, Maria LU and Mosbach, Klaus LU (1991) In Analytical Letters 24(7). p.1137-1145
Abstract
A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol... (More)
A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol per g dry polymer. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Letters
volume
24
issue
7
pages
1137 - 1145
publisher
Taylor & Francis
external identifiers
  • scopus:0000665502
ISSN
0003-2719
DOI
10.1080/00032719108052959
language
English
LU publication?
yes
id
197029d0-7438-4e4f-a4a3-1f197ff20c31 (old id 1516052)
date added to LUP
2009-12-17 08:35:13
date last changed
2017-07-30 04:30:39
@article{197029d0-7438-4e4f-a4a3-1f197ff20c31,
  abstract     = {A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol per g dry polymer.},
  author       = {Kempe, Maria and Mosbach, Klaus},
  issn         = {0003-2719},
  language     = {eng},
  number       = {7},
  pages        = {1137--1145},
  publisher    = {Taylor & Francis},
  series       = {Analytical Letters},
  title        = {Binding studies on substrate- and enantio-selective molecularly imprinted polymers},
  url          = {http://dx.doi.org/10.1080/00032719108052959},
  volume       = {24},
  year         = {1991},
}