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A comparison between batch and fed-batch simultaneous saccharification and fermentation of steam pretreated spruce

Rudolf, Andreas LU ; Alkasrawi, Malek LU ; Zacchi, Guido LU and Lidén, Gunnar LU (2005) In Enzyme and Microbial Technology 37(2). p.195-204
Abstract
In order to improve the process economy it is important to use as high dry matter content as possible in simultaneous saccharification and fermentation (SSF). However, too high dry matter content often gives rise to severe inhibition of the yeast metabolism, due to the increased levels of toxic compounds. The aim of the present work was to increase the fibrous content in SSF of steam pretreated spruce to 10% by adapting the yeast to the inhibitory substrate and by using a fed-batch process. Both batch and fed-batch approaches were evaluated. The fed-batch experiments were started with a batch fermentation containing 6% dry matter. Fibrous slurry from the pretreatment was then added four times during the first 24 h giving a final dry matter... (More)
In order to improve the process economy it is important to use as high dry matter content as possible in simultaneous saccharification and fermentation (SSF). However, too high dry matter content often gives rise to severe inhibition of the yeast metabolism, due to the increased levels of toxic compounds. The aim of the present work was to increase the fibrous content in SSF of steam pretreated spruce to 10% by adapting the yeast to the inhibitory substrate and by using a fed-batch process. Both batch and fed-batch approaches were evaluated. The fed-batch experiments were started with a batch fermentation containing 6% dry matter. Fibrous slurry from the pretreatment was then added four times during the first 24 h giving a final dry matter content corresponding to 10%. The yeast used in the fermentation was produced aerobically on the hemicellulose hydrolysate obtained from the pretreatment. SSF batch and fed-batch experiments with a cell mass concentration of 2, 3 and 5 g/L were carried out. When adapted yeast was used, the available hexoses were completely converted within 72 h and the final ethanol concentrations reached 40-44 g/L. No major differences in performance between batch and fed-batch were seen, but the ethanol productivity during the first 24 h was higher in the fed-batch SSF experiments, particularly during the experiments with a cell mass concentration of 2 and 3 g/L. (c) 2005 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Enzyme and Microbial Technology
volume
37
issue
2
pages
195 - 204
publisher
Elsevier
external identifiers
  • wos:000229500800005
  • scopus:18944392490
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2005.02.013
language
English
LU publication?
yes
id
8e47c110-b011-40b6-9127-af2edf1ab7eb (old id 151697)
date added to LUP
2007-06-25 15:48:55
date last changed
2017-07-23 03:37:06
@article{8e47c110-b011-40b6-9127-af2edf1ab7eb,
  abstract     = {In order to improve the process economy it is important to use as high dry matter content as possible in simultaneous saccharification and fermentation (SSF). However, too high dry matter content often gives rise to severe inhibition of the yeast metabolism, due to the increased levels of toxic compounds. The aim of the present work was to increase the fibrous content in SSF of steam pretreated spruce to 10% by adapting the yeast to the inhibitory substrate and by using a fed-batch process. Both batch and fed-batch approaches were evaluated. The fed-batch experiments were started with a batch fermentation containing 6% dry matter. Fibrous slurry from the pretreatment was then added four times during the first 24 h giving a final dry matter content corresponding to 10%. The yeast used in the fermentation was produced aerobically on the hemicellulose hydrolysate obtained from the pretreatment. SSF batch and fed-batch experiments with a cell mass concentration of 2, 3 and 5 g/L were carried out. When adapted yeast was used, the available hexoses were completely converted within 72 h and the final ethanol concentrations reached 40-44 g/L. No major differences in performance between batch and fed-batch were seen, but the ethanol productivity during the first 24 h was higher in the fed-batch SSF experiments, particularly during the experiments with a cell mass concentration of 2 and 3 g/L. (c) 2005 Elsevier Inc. All rights reserved.},
  author       = {Rudolf, Andreas and Alkasrawi, Malek and Zacchi, Guido and Lidén, Gunnar},
  issn         = {0141-0229},
  language     = {eng},
  number       = {2},
  pages        = {195--204},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {A comparison between batch and fed-batch simultaneous saccharification and fermentation of steam pretreated spruce},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2005.02.013},
  volume       = {37},
  year         = {2005},
}