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Vascular Endothelin and Angiotensin Receptors Regulation in Inflammatory Arterial Disorders

Dimitrijevic, Ivan LU (2010) In Lund University, Faculty of Medicine Doctoral Dissertation Series 2010:2.
Abstract
The present thesis is aimed to examine the hypothesis that the degree of vascular inflammation correlates with the expression of vascular endothelin and angiotensin receptors. The receptor changes were studied in subcutaneous resistance arteries in patients with different degrees of ischemic heart disease (IHD). In addition, patients with giant cell arteritis (GCA) were also investigated because of the massive inflammatory activity in affected vessels. For functional studies of the resistance arteries, sensitive myographs were used. Receptor protein levels were measured quantitatively using immunohistochemistry or western blot.

Main results: 1. Patients with suspected acute coronary syndrome (angina pectoris) have increased... (More)
The present thesis is aimed to examine the hypothesis that the degree of vascular inflammation correlates with the expression of vascular endothelin and angiotensin receptors. The receptor changes were studied in subcutaneous resistance arteries in patients with different degrees of ischemic heart disease (IHD). In addition, patients with giant cell arteritis (GCA) were also investigated because of the massive inflammatory activity in affected vessels. For functional studies of the resistance arteries, sensitive myographs were used. Receptor protein levels were measured quantitatively using immunohistochemistry or western blot.

Main results: 1. Patients with suspected acute coronary syndrome (angina pectoris) have increased expression of AT1 and ETB receptors in vascular smooth muscle cells (VSMC) in subcutaneous resistance arteries. 2. The

level of the up-regulation of contractile AT1 and ETB receptors depends on the degree of underlying IHD. The phenomenon is also correlated to the level of the systolic blood pressure. 3. Temporal arteries from patients

with GCA have up-regulation of AT1 and ETB receptors in the VSMC in the medial layer of the arterial wall. The degrees of up-regulation of ETB receptors are directly correlated to the degree of the systemic

inflammatory response (CRP). 4. Accompanying lymphocytic infiltrates as well as giant cells in the lesions of the temporal arteries express high immunostaining of endothelin-1 and up-regulation of ETB and AT1

receptors as well. 5. De novo expression of tissue endothelin-1 was observed in the VSMC in the medial layer as well in the neointima in affected arteries. 6. ETB receptors mediate contraction of subcutaneous resistance arteries in ischemic heart disease.

The present thesis demonstrated that there is increased ETB and AT1 receptor expression in VSMC in ischemic heart disease and in GCA which correlates with the degree of inflammation. (Less)
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author
supervisor
opponent
  • Assistant Professor Nordborg, Claes, Department of Pathology, Gothenburg University, Gothenburg, Sweden
organization
publishing date
type
Thesis
publication status
published
subject
keywords
ischemic heart disease, resistance artery, giant cell arteritis, temporal artery, immunohistochemistry, AT2 receptor, AT1 receptor, Endothelin-1, ETA receptor, ETB receptor
in
Lund University, Faculty of Medicine Doctoral Dissertation Series
volume
2010:2
pages
102 pages
publisher
Department of Internal Medcine, University Hospital of Lund,
defense location
Segerfalkssalen, Wallenberg Neuroscience Centre, Lund University, Sweden
defense date
2010-01-22 13:00
ISSN
1652-8220
ISBN
978-91-86443-16-0
language
English
LU publication?
yes
id
1359e263-370d-496a-b161-5049cd3ed3de (old id 1517145)
date added to LUP
2010-01-05 14:46:01
date last changed
2018-05-29 11:23:26
@phdthesis{1359e263-370d-496a-b161-5049cd3ed3de,
  abstract     = {The present thesis is aimed to examine the hypothesis that the degree of vascular inflammation correlates with the expression of vascular endothelin and angiotensin receptors. The receptor changes were studied in subcutaneous resistance arteries in patients with different degrees of ischemic heart disease (IHD). In addition, patients with giant cell arteritis (GCA) were also investigated because of the massive inflammatory activity in affected vessels. For functional studies of the resistance arteries, sensitive myographs were used. Receptor protein levels were measured quantitatively using immunohistochemistry or western blot.<br/><br>
Main results: 1. Patients with suspected acute coronary syndrome (angina pectoris) have increased expression of AT1 and ETB receptors in vascular smooth muscle cells (VSMC) in subcutaneous resistance arteries. 2. The<br/><br>
level of the up-regulation of contractile AT1 and ETB receptors depends on the degree of underlying IHD. The phenomenon is also correlated to the level of the systolic blood pressure. 3. Temporal arteries from patients<br/><br>
with GCA have up-regulation of AT1 and ETB receptors in the VSMC in the medial layer of the arterial wall. The degrees of up-regulation of ETB receptors are directly correlated to the degree of the systemic<br/><br>
inflammatory response (CRP). 4. Accompanying lymphocytic infiltrates as well as giant cells in the lesions of the temporal arteries express high immunostaining of endothelin-1 and up-regulation of ETB and AT1<br/><br>
receptors as well. 5. De novo expression of tissue endothelin-1 was observed in the VSMC in the medial layer as well in the neointima in affected arteries. 6. ETB receptors mediate contraction of subcutaneous resistance arteries in ischemic heart disease.<br/><br>
The present thesis demonstrated that there is increased ETB and AT1 receptor expression in VSMC in ischemic heart disease and in GCA which correlates with the degree of inflammation.},
  author       = {Dimitrijevic, Ivan},
  isbn         = {978-91-86443-16-0},
  issn         = {1652-8220},
  keyword      = {ischemic heart disease,resistance artery,giant cell arteritis,temporal artery,immunohistochemistry,AT2 receptor,AT1 receptor,Endothelin-1,ETA receptor,ETB receptor},
  language     = {eng},
  pages        = {102},
  publisher    = {Department of Internal Medcine, University Hospital of Lund,},
  school       = {Lund University},
  series       = {Lund University, Faculty of Medicine Doctoral Dissertation Series},
  title        = {Vascular Endothelin and Angiotensin Receptors Regulation in Inflammatory Arterial Disorders},
  volume       = {2010:2},
  year         = {2010},
}