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Estimation of adsorption parameters in a detailed affinity chromatography model based on shallow bed experiments

Persson, Patrik LU ; Kempe, Henrik LU ; Zacchi, Guido LU and Nilsson, Bernt LU (2005) In Process Biochemistry 40(5). p.1649-1659
Abstract
A method for estimation of unknown adsorption parameters in a detailed chromatography model including the Langmuir adsorption expression is proposed. The model describes the concentration of the solute in the mobile phase and considers extemal/intemal mass transfer resistance and adsorption dynamics. The external volume and its mixing effect are also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient, the apparent bead porosity, the adsorption/desorption rate coefficients and the Langmuir isotherm. All the parameters in the model were subsequently determined in four classes of experiments, the external mixing... (More)
A method for estimation of unknown adsorption parameters in a detailed chromatography model including the Langmuir adsorption expression is proposed. The model describes the concentration of the solute in the mobile phase and considers extemal/intemal mass transfer resistance and adsorption dynamics. The external volume and its mixing effect are also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient, the apparent bead porosity, the adsorption/desorption rate coefficients and the Langmuir isotherm. All the parameters in the model were subsequently determined in four classes of experiments, the external mixing behaviour, the mobile phase behaviour, the stationary phase behaviour and the adsorption behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with bovine serum albumin in a shallow packed bed. The results show that it is possible to estimate the adsorption parameters using the proposed methodology, and to replace the Langmuir adsorption dynamic expression with the corresponding isotherm when high inlet concentrations are used. It was also investigated at what inlet concentration this model simplification resulted in satisfactory results. It was possible to estimate adsorption parameters using several experimental breakthrough curves simultaneously, i.e. multiple data responses, to obtain single parameters that are valid within the interval of inlet concentrations used. (c) 2004 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Process Biochemistry
volume
40
issue
5
pages
1649 - 1659
publisher
Elsevier
external identifiers
  • wos:000227569000023
  • scopus:13844272469
ISSN
1873-3298
DOI
10.1016/j.procbio.2004.06.031
language
English
LU publication?
yes
id
fd6a2fe2-9250-4c08-9271-bf67ab08b668 (old id 151733)
date added to LUP
2007-06-25 15:31:20
date last changed
2017-01-01 05:09:30
@article{fd6a2fe2-9250-4c08-9271-bf67ab08b668,
  abstract     = {A method for estimation of unknown adsorption parameters in a detailed chromatography model including the Langmuir adsorption expression is proposed. The model describes the concentration of the solute in the mobile phase and considers extemal/intemal mass transfer resistance and adsorption dynamics. The external volume and its mixing effect are also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient, the apparent bead porosity, the adsorption/desorption rate coefficients and the Langmuir isotherm. All the parameters in the model were subsequently determined in four classes of experiments, the external mixing behaviour, the mobile phase behaviour, the stationary phase behaviour and the adsorption behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with bovine serum albumin in a shallow packed bed. The results show that it is possible to estimate the adsorption parameters using the proposed methodology, and to replace the Langmuir adsorption dynamic expression with the corresponding isotherm when high inlet concentrations are used. It was also investigated at what inlet concentration this model simplification resulted in satisfactory results. It was possible to estimate adsorption parameters using several experimental breakthrough curves simultaneously, i.e. multiple data responses, to obtain single parameters that are valid within the interval of inlet concentrations used. (c) 2004 Elsevier Ltd. All rights reserved.},
  author       = {Persson, Patrik and Kempe, Henrik and Zacchi, Guido and Nilsson, Bernt},
  issn         = {1873-3298},
  language     = {eng},
  number       = {5},
  pages        = {1649--1659},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Estimation of adsorption parameters in a detailed affinity chromatography model based on shallow bed experiments},
  url          = {http://dx.doi.org/10.1016/j.procbio.2004.06.031},
  volume       = {40},
  year         = {2005},
}