Advanced

Neutralizing activity and cellular immune responses induced in mice after immunization with apoptotic HIV-1/murine leukemia virus infected cells

Hinkula, Jorma; Walther-Jallow, Lilian; Laurén, Anna LU ; Makitalo, Barbro; Öberg, Monica LU ; Wahren, Britta; Fenyö, Eva Maria LU and Spetz, Anna-Lena (2009) In Vaccine 27(46). p.6424-6431
Abstract
Dendritic cells present microbial antigens to T cells after uptake of apoptotic vesicles from infected cells. We previously reported that immunizations with apoptotic HIV-1/murine leukemia virus (MuLV) infected cells lead to induction of both cellular and humoral immune responses as well as resistance to mucosal challenge with live HIV-1/MuLV infected cells. Here we extended those studies and investigated whether apoptotic cells from HIV-1/MuLV infected cells stimulate the production of HIV-1 neutralizing activity. We compared different routes of administration and were able to induce p24- and Nef-specific cellular proliferation after intraperitoneal (i.p.), intranasal (i.n.), subcutaneous (s.c.) and intramuscular (i.m.) immunizations.... (More)
Dendritic cells present microbial antigens to T cells after uptake of apoptotic vesicles from infected cells. We previously reported that immunizations with apoptotic HIV-1/murine leukemia virus (MuLV) infected cells lead to induction of both cellular and humoral immune responses as well as resistance to mucosal challenge with live HIV-1/MuLV infected cells. Here we extended those studies and investigated whether apoptotic cells from HIV-1/MuLV infected cells stimulate the production of HIV-1 neutralizing activity. We compared different routes of administration and were able to induce p24- and Nef-specific cellular proliferation after intraperitoneal (i.p.), intranasal (i.n.), subcutaneous (s.c.) and intramuscular (i.m.) immunizations. Serum IgG and IgA antibodies directed against gp160, p24, or Nef were also produced regardless of immunization route used. However, the induction of mucosa-associated IgAs from faeces or vaginal secretions were detected only after either i.p. or i.n. immunizations. We were able to measure neutralizing activity in sera of mice after i.p. and i.n. immunization. Neutralizing reactivity was also detected after s.c. and i.m. immunizations in the presence of the cytokine adjuvant granulocyte macrophage-colony stimulating factor (GM-CSF). Conclusively we show induction of cellular and humoral immune responses including neutralizing activity after immunization with apoptotic HIV-1/MuLV infected cells in mice. The results from this study support further evaluations using apoptotic cells as antigen delivery system for vaccination against HIV-1 in other animal models. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immunization, routes, B cells, T cells, Apoptosis, HIV-1, Virus neutralization
in
Vaccine
volume
27
issue
46
pages
6424 - 6431
publisher
Elsevier
external identifiers
  • wos:000271922700008
  • scopus:70350072669
ISSN
1873-2518
DOI
10.1016/j.vaccine.2009.06.016
language
English
LU publication?
yes
id
39f624f1-bc39-4f62-af42-f932cbd81c34 (old id 1517894)
date added to LUP
2010-01-08 13:21:09
date last changed
2017-01-01 04:30:34
@article{39f624f1-bc39-4f62-af42-f932cbd81c34,
  abstract     = {Dendritic cells present microbial antigens to T cells after uptake of apoptotic vesicles from infected cells. We previously reported that immunizations with apoptotic HIV-1/murine leukemia virus (MuLV) infected cells lead to induction of both cellular and humoral immune responses as well as resistance to mucosal challenge with live HIV-1/MuLV infected cells. Here we extended those studies and investigated whether apoptotic cells from HIV-1/MuLV infected cells stimulate the production of HIV-1 neutralizing activity. We compared different routes of administration and were able to induce p24- and Nef-specific cellular proliferation after intraperitoneal (i.p.), intranasal (i.n.), subcutaneous (s.c.) and intramuscular (i.m.) immunizations. Serum IgG and IgA antibodies directed against gp160, p24, or Nef were also produced regardless of immunization route used. However, the induction of mucosa-associated IgAs from faeces or vaginal secretions were detected only after either i.p. or i.n. immunizations. We were able to measure neutralizing activity in sera of mice after i.p. and i.n. immunization. Neutralizing reactivity was also detected after s.c. and i.m. immunizations in the presence of the cytokine adjuvant granulocyte macrophage-colony stimulating factor (GM-CSF). Conclusively we show induction of cellular and humoral immune responses including neutralizing activity after immunization with apoptotic HIV-1/MuLV infected cells in mice. The results from this study support further evaluations using apoptotic cells as antigen delivery system for vaccination against HIV-1 in other animal models. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Hinkula, Jorma and Walther-Jallow, Lilian and Laurén, Anna and Makitalo, Barbro and Öberg, Monica and Wahren, Britta and Fenyö, Eva Maria and Spetz, Anna-Lena},
  issn         = {1873-2518},
  keyword      = {Immunization,routes,B cells,T cells,Apoptosis,HIV-1,Virus neutralization},
  language     = {eng},
  number       = {46},
  pages        = {6424--6431},
  publisher    = {Elsevier},
  series       = {Vaccine},
  title        = {Neutralizing activity and cellular immune responses induced in mice after immunization with apoptotic HIV-1/murine leukemia virus infected cells},
  url          = {http://dx.doi.org/10.1016/j.vaccine.2009.06.016},
  volume       = {27},
  year         = {2009},
}