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Bacterial and fungal growth in soil heated at different temperatures to simulate a range of fire intensities

Barcenas-Moreno, Gema and Bååth, Erland LU (2009) In Soil Biology & Biochemistry 41(12). p.2517-2526
Abstract
The intensity of a fire is an important factor determining the recovery of soil microorganisms after a forest fire, since it can alter the quality and quantity of carbon sources. Recovery of the microbial community was studied in a Mediterranean pine forest soil subjected to different temperatures to simulate the short-term effects of fire intensity on bacterial and fungal growth, estimated using leucine incorporation for bacteria and acetate incorporation into ergosterol for fungi. Soil samples were heated for 15 min at 50, 80,120, 200, 300, 400 and 500 degrees C. After inoculation with fresh soil, and adding water to achieve 60% WHC, the soils were incubated at 20 degrees C for 21 days. Bacterial growth was initially inhibited in the... (More)
The intensity of a fire is an important factor determining the recovery of soil microorganisms after a forest fire, since it can alter the quality and quantity of carbon sources. Recovery of the microbial community was studied in a Mediterranean pine forest soil subjected to different temperatures to simulate the short-term effects of fire intensity on bacterial and fungal growth, estimated using leucine incorporation for bacteria and acetate incorporation into ergosterol for fungi. Soil samples were heated for 15 min at 50, 80,120, 200, 300, 400 and 500 degrees C. After inoculation with fresh soil, and adding water to achieve 60% WHC, the soils were incubated at 20 degrees C for 21 days. Bacterial growth was initially inhibited in the samples heated above 50 degrees C (totally inhibited >= 200 degrees C), but recovered within days to levels much higher than the control, except for the samples heated at 500 degrees C, where growth remained low throughout the incubation period due to the destruction of most of the organic matter. After the first week of incubation, the bacterial response decreased to values close to, but still above, that of the control. Samples heated at 200 degrees C showed the highest cumulative bacterial growth. Fungal growth was initially lower than in the control in all the heated samples (totally inhibited >= 200 degrees C). Fungal growth recovered slowly during incubation in soils heated at <= 300 degrees C, but the cumulative growth in heated soils did not exceed that in the control. No fungal growth was observed in samples heated at the two highest temperatures. Soil respiration was initially totally inhibited in soil heated at >= 200 degrees C, but recovered rapidly in all soils; the highest respiration being observed already 1 day after inoculation. This is the first time both fungal and bacterial growth has been directly estimated in heated soils. High soil pH favouring bacteria can explain these results, but the differences in fungal and bacterial responses suggest a competitive interaction between these groups. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacteria, Fungi, Ac-in-erg, Forest fire, Leu, Growth rates, PLFA
in
Soil Biology & Biochemistry
volume
41
issue
12
pages
2517 - 2526
publisher
Elsevier
external identifiers
  • wos:000272062100018
  • scopus:70350571795
ISSN
0038-0717
DOI
10.1016/j.soilbio.2009.09.010
language
English
LU publication?
yes
id
e6806cac-51c7-40e3-ad0a-0b693f917af7 (old id 1517928)
date added to LUP
2010-01-13 11:49:13
date last changed
2017-10-01 04:16:49
@article{e6806cac-51c7-40e3-ad0a-0b693f917af7,
  abstract     = {The intensity of a fire is an important factor determining the recovery of soil microorganisms after a forest fire, since it can alter the quality and quantity of carbon sources. Recovery of the microbial community was studied in a Mediterranean pine forest soil subjected to different temperatures to simulate the short-term effects of fire intensity on bacterial and fungal growth, estimated using leucine incorporation for bacteria and acetate incorporation into ergosterol for fungi. Soil samples were heated for 15 min at 50, 80,120, 200, 300, 400 and 500 degrees C. After inoculation with fresh soil, and adding water to achieve 60% WHC, the soils were incubated at 20 degrees C for 21 days. Bacterial growth was initially inhibited in the samples heated above 50 degrees C (totally inhibited &gt;= 200 degrees C), but recovered within days to levels much higher than the control, except for the samples heated at 500 degrees C, where growth remained low throughout the incubation period due to the destruction of most of the organic matter. After the first week of incubation, the bacterial response decreased to values close to, but still above, that of the control. Samples heated at 200 degrees C showed the highest cumulative bacterial growth. Fungal growth was initially lower than in the control in all the heated samples (totally inhibited &gt;= 200 degrees C). Fungal growth recovered slowly during incubation in soils heated at &lt;= 300 degrees C, but the cumulative growth in heated soils did not exceed that in the control. No fungal growth was observed in samples heated at the two highest temperatures. Soil respiration was initially totally inhibited in soil heated at &gt;= 200 degrees C, but recovered rapidly in all soils; the highest respiration being observed already 1 day after inoculation. This is the first time both fungal and bacterial growth has been directly estimated in heated soils. High soil pH favouring bacteria can explain these results, but the differences in fungal and bacterial responses suggest a competitive interaction between these groups. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Barcenas-Moreno, Gema and Bååth, Erland},
  issn         = {0038-0717},
  keyword      = {Bacteria,Fungi,Ac-in-erg,Forest fire,Leu,Growth rates,PLFA},
  language     = {eng},
  number       = {12},
  pages        = {2517--2526},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Bacterial and fungal growth in soil heated at different temperatures to simulate a range of fire intensities},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2009.09.010},
  volume       = {41},
  year         = {2009},
}