Optical microscopy in photosynthesis
Cisek, Richard ; Spencer, Leigh ; Prent, Nicole ; Zigmantas, Donatas LU
; Espie, George S.
and Barzda, Virginijus
(2009)
In Photosynthesis Research
102(2-3).
p.111-141
- Abstract
- Emerging as well as the most frequently used optical microscopy techniques are reviewed and image contrast generation methods in a microscope are presented, focusing on the nonlinear contrasts such as harmonic generation and multiphoton excitation fluorescence. Nonlinear microscopy presents numerous advantages over linear microscopy techniques including improved deep tissue imaging, optical sectioning, and imaging of live unstained samples. Nonetheless, with the exception of multiphoton excitation fluorescence, nonlinear microscopy is in its infancy, lacking protocols, users and applications; hence, this review focuses on the potential of nonlinear microscopy for studying photosynthetic organisms. Examples of nonlinear microscopic imaging... (More)
- Emerging as well as the most frequently used optical microscopy techniques are reviewed and image contrast generation methods in a microscope are presented, focusing on the nonlinear contrasts such as harmonic generation and multiphoton excitation fluorescence. Nonlinear microscopy presents numerous advantages over linear microscopy techniques including improved deep tissue imaging, optical sectioning, and imaging of live unstained samples. Nonetheless, with the exception of multiphoton excitation fluorescence, nonlinear microscopy is in its infancy, lacking protocols, users and applications; hence, this review focuses on the potential of nonlinear microscopy for studying photosynthetic organisms. Examples of nonlinear microscopic imaging are presented including isolated light-harvesting antenna complexes from higher plants, starch granules, chloroplasts, unicellular alga Chlamydomonas reinhardtii, and cyanobacteria Leptolyngbya sp. and Anabaena sp. While focusing on nonlinear microscopy techniques, second and third harmonic generation and multiphoton excitation fluorescence microscopy, other emerging nonlinear imaging modalities are described and several linear optical microscopy techniques are reviewed in order to clearly describe their capabilities and to highlight the advantages of nonlinear microscopy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1518734
- author
- Cisek, Richard
; Spencer, Leigh
; Prent, Nicole
; Zigmantas, Donatas
LU
; Espie, George S.
and Barzda, Virginijus
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chlamydomonas, generation, Third harmonic, Nonlinear microscopy, Second harmonic generation, Chloroplasts, Cyanobacteria
- in
- Photosynthesis Research
- volume
- 102
- issue
- 2-3
- pages
- 111 - 141
- publisher
- Springer
- external identifiers
-
- wos:000271795400003
- scopus:76149139412
- pmid:19851883
- ISSN
- 0166-8595
- DOI
- 10.1007/s11120-009-9500-9
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
- id
- 72365148-62da-481a-96b9-9869c453a4b0 (old id 1518734)
- date added to LUP
- 2016-04-01 14:24:22
- date last changed
- 2022-03-14 05:42:17
@article{72365148-62da-481a-96b9-9869c453a4b0,
abstract = {{Emerging as well as the most frequently used optical microscopy techniques are reviewed and image contrast generation methods in a microscope are presented, focusing on the nonlinear contrasts such as harmonic generation and multiphoton excitation fluorescence. Nonlinear microscopy presents numerous advantages over linear microscopy techniques including improved deep tissue imaging, optical sectioning, and imaging of live unstained samples. Nonetheless, with the exception of multiphoton excitation fluorescence, nonlinear microscopy is in its infancy, lacking protocols, users and applications; hence, this review focuses on the potential of nonlinear microscopy for studying photosynthetic organisms. Examples of nonlinear microscopic imaging are presented including isolated light-harvesting antenna complexes from higher plants, starch granules, chloroplasts, unicellular alga Chlamydomonas reinhardtii, and cyanobacteria Leptolyngbya sp. and Anabaena sp. While focusing on nonlinear microscopy techniques, second and third harmonic generation and multiphoton excitation fluorescence microscopy, other emerging nonlinear imaging modalities are described and several linear optical microscopy techniques are reviewed in order to clearly describe their capabilities and to highlight the advantages of nonlinear microscopy.}},
author = {{Cisek, Richard and Spencer, Leigh and Prent, Nicole and Zigmantas, Donatas and Espie, George S. and Barzda, Virginijus}},
issn = {{0166-8595}},
keywords = {{Chlamydomonas; generation; Third harmonic; Nonlinear microscopy; Second harmonic generation; Chloroplasts; Cyanobacteria}},
language = {{eng}},
number = {{2-3}},
pages = {{111--141}},
publisher = {{Springer}},
series = {{Photosynthesis Research}},
title = {{Optical microscopy in photosynthesis}},
url = {{http://dx.doi.org/10.1007/s11120-009-9500-9}},
doi = {{10.1007/s11120-009-9500-9}},
volume = {{102}},
year = {{2009}},
}