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Subarachnoid Hemorrhage Induces Enhanced Expression of Thromboxane A(2) Receptors in Rat Cerebral Arteries.

Ansar, Saema LU ; Larsen, Carl; Maddahi, Aida LU and Edvinsson, Lars LU (2010) In Brain Research1966-01-01+01:00 1316. p.163-172
Abstract
Cerebral ischemia remains the key cause of morbidity and mortality after subarachnoid hemorrhage (SAH) with a pathogenesis that is still poorly understood. The aim of the present study was to examine the involvement of thromboxane A(2) receptors (TP) in the patophysiology of cerebral ischemia after SAH in cerebral arteries. SAH was induced in rats by injecting 250 microl blood into the prechiasmatic cistern. Two days after the SAH, cerebral arteries were harvested and contractile responses to the TP receptor agonist U46619 were investigated with myographs. In addition, the contractile responses were examined after pretreatment with selective TP receptor antagonist GR3219b. The TP receptor RNA and protein levels were analyzed by... (More)
Cerebral ischemia remains the key cause of morbidity and mortality after subarachnoid hemorrhage (SAH) with a pathogenesis that is still poorly understood. The aim of the present study was to examine the involvement of thromboxane A(2) receptors (TP) in the patophysiology of cerebral ischemia after SAH in cerebral arteries. SAH was induced in rats by injecting 250 microl blood into the prechiasmatic cistern. Two days after the SAH, cerebral arteries were harvested and contractile responses to the TP receptor agonist U46619 were investigated with myographs. In addition, the contractile responses were examined after pretreatment with selective TP receptor antagonist GR3219b. The TP receptor RNA and protein levels were analyzed by quantitative real-time PCR and immunohistochemistry, respectively. The global and regional cerebral blood flows (CBF) were quantified with an autoradiographic technique. SAH resulted in enhanced contractile responses to U46619 as compared to sham. The TP receptor antagonist GR3219b abolished the enhanced contractile responses to U46619 observed after SAH. The TP receptor mRNA level was elevated after SAH as compared to sham. The level of TP receptor protein on the smooth muscle cells (SMC) was increased in SAH compared to sham, Global and regional CBF was reduced in SAH as compared to sham. The results demonstrate that SAH results in CBF reduction and this is associated with enhanced expression of TP receptors in the SMC of cerebral arteries and microvessels. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Brain Research1966-01-01+01:00
volume
1316
pages
163 - 172
publisher
Elsevier
external identifiers
  • wos:000275136200018
  • pmid:20026315
  • scopus:75449106951
ISSN
1872-6240
DOI
10.1016/j.brainres.2009.12.031
language
English
LU publication?
yes
id
78d21642-3dee-4227-a0bf-d605959ce3aa (old id 1523415)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20026315?dopt=Abstract
date added to LUP
2010-01-11 16:59:28
date last changed
2018-05-29 10:27:44
@article{78d21642-3dee-4227-a0bf-d605959ce3aa,
  abstract     = {Cerebral ischemia remains the key cause of morbidity and mortality after subarachnoid hemorrhage (SAH) with a pathogenesis that is still poorly understood. The aim of the present study was to examine the involvement of thromboxane A(2) receptors (TP) in the patophysiology of cerebral ischemia after SAH in cerebral arteries. SAH was induced in rats by injecting 250 microl blood into the prechiasmatic cistern. Two days after the SAH, cerebral arteries were harvested and contractile responses to the TP receptor agonist U46619 were investigated with myographs. In addition, the contractile responses were examined after pretreatment with selective TP receptor antagonist GR3219b. The TP receptor RNA and protein levels were analyzed by quantitative real-time PCR and immunohistochemistry, respectively. The global and regional cerebral blood flows (CBF) were quantified with an autoradiographic technique. SAH resulted in enhanced contractile responses to U46619 as compared to sham. The TP receptor antagonist GR3219b abolished the enhanced contractile responses to U46619 observed after SAH. The TP receptor mRNA level was elevated after SAH as compared to sham. The level of TP receptor protein on the smooth muscle cells (SMC) was increased in SAH compared to sham, Global and regional CBF was reduced in SAH as compared to sham. The results demonstrate that SAH results in CBF reduction and this is associated with enhanced expression of TP receptors in the SMC of cerebral arteries and microvessels.},
  author       = {Ansar, Saema and Larsen, Carl and Maddahi, Aida and Edvinsson, Lars},
  issn         = {1872-6240},
  language     = {eng},
  pages        = {163--172},
  publisher    = {Elsevier},
  series       = {Brain Research1966-01-01+01:00},
  title        = {Subarachnoid Hemorrhage Induces Enhanced Expression of Thromboxane A(2) Receptors in Rat Cerebral Arteries.},
  url          = {http://dx.doi.org/10.1016/j.brainres.2009.12.031},
  volume       = {1316},
  year         = {2010},
}