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Release of DNA from surfactant complexes induced by 2-hydroxypropyl-beta-cyclodextrin.

Carlstedt, Jonas LU ; Gonzalez Perez, Alfredo LU ; Alatorre Meda, Manuel LU ; Dias, Rita LU and Lindman, Björn LU (2010) In International Journal of Biological Macromolecules 46(2). p.153-158
Abstract
Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescence microscopy, steady state fluorescence spectroscopy, density and sound velocity measurements, thermal melting and circular dichroism were used. Compared to previous work using alpha- and beta-CD, the fluorescence spectroscopy results showed that the 2-HP-substituted CDs more efficiently released DNA into solution. Furthermore, dissociation of macroscopically phase separated DNA-CTA complexes was achieved upon addition of... (More)
Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescence microscopy, steady state fluorescence spectroscopy, density and sound velocity measurements, thermal melting and circular dichroism were used. Compared to previous work using alpha- and beta-CD, the fluorescence spectroscopy results showed that the 2-HP-substituted CDs more efficiently released DNA into solution. Furthermore, dissociation of macroscopically phase separated DNA-CTA complexes was achieved upon addition of 2-HP-beta-CD and the results gave strong indications on the non-equilibrium nature of the system. The globule-to-coil transition was not found to proceed through a coexistence region, which seems to be a general phenomenon in DNA decompaction using CDs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Biological Macromolecules
volume
46
issue
2
pages
153 - 158
publisher
Elsevier
external identifiers
  • wos:000275700900004
  • pmid:20025900
  • scopus:77949320835
ISSN
1879-0003
DOI
10.1016/j.ijbiomac.2009.12.002
language
English
LU publication?
yes
id
4a7ef840-6f9b-4820-9fde-09d15bf95bbb (old id 1523422)
alternative location
http://www.sciencedirect.com/science/article/B6T7J-4XY4GM3-1/2/940c9723db4e55f3381879994375ae51
date added to LUP
2010-01-12 07:47:02
date last changed
2018-05-29 10:26:30
@article{4a7ef840-6f9b-4820-9fde-09d15bf95bbb,
  abstract     = {Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescence microscopy, steady state fluorescence spectroscopy, density and sound velocity measurements, thermal melting and circular dichroism were used. Compared to previous work using alpha- and beta-CD, the fluorescence spectroscopy results showed that the 2-HP-substituted CDs more efficiently released DNA into solution. Furthermore, dissociation of macroscopically phase separated DNA-CTA complexes was achieved upon addition of 2-HP-beta-CD and the results gave strong indications on the non-equilibrium nature of the system. The globule-to-coil transition was not found to proceed through a coexistence region, which seems to be a general phenomenon in DNA decompaction using CDs.},
  author       = {Carlstedt, Jonas and Gonzalez Perez, Alfredo and Alatorre Meda, Manuel and Dias, Rita and Lindman, Björn},
  issn         = {1879-0003},
  language     = {eng},
  number       = {2},
  pages        = {153--158},
  publisher    = {Elsevier},
  series       = {International Journal of Biological Macromolecules},
  title        = {Release of DNA from surfactant complexes induced by 2-hydroxypropyl-beta-cyclodextrin.},
  url          = {http://dx.doi.org/10.1016/j.ijbiomac.2009.12.002},
  volume       = {46},
  year         = {2010},
}