Release of DNA from surfactant complexes induced by 2-hydroxypropyl-beta-cyclodextrin.
(2010) In International Journal of Biological Macromolecules 46(2). p.153-158- Abstract
- Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescence microscopy, steady state fluorescence spectroscopy, density and sound velocity measurements, thermal melting and circular dichroism were used. Compared to previous work using alpha- and beta-CD, the fluorescence spectroscopy results showed that the 2-HP-substituted CDs more efficiently released DNA into solution. Furthermore, dissociation of macroscopically phase separated DNA-CTA complexes was achieved upon addition of... (More)
- Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescence microscopy, steady state fluorescence spectroscopy, density and sound velocity measurements, thermal melting and circular dichroism were used. Compared to previous work using alpha- and beta-CD, the fluorescence spectroscopy results showed that the 2-HP-substituted CDs more efficiently released DNA into solution. Furthermore, dissociation of macroscopically phase separated DNA-CTA complexes was achieved upon addition of 2-HP-beta-CD and the results gave strong indications on the non-equilibrium nature of the system. The globule-to-coil transition was not found to proceed through a coexistence region, which seems to be a general phenomenon in DNA decompaction using CDs. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1523422
- author
- Carlstedt, Jonas LU ; Gonzalez Perez, Alfredo LU ; Alatorre Meda, Manuel LU ; Dias, Rita LU and Lindman, Björn LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Biological Macromolecules
- volume
- 46
- issue
- 2
- pages
- 153 - 158
- publisher
- Elsevier
- external identifiers
-
- wos:000275700900004
- pmid:20025900
- scopus:77949320835
- pmid:20025900
- ISSN
- 1879-0003
- DOI
- 10.1016/j.ijbiomac.2009.12.002
- language
- English
- LU publication?
- yes
- id
- 4a7ef840-6f9b-4820-9fde-09d15bf95bbb (old id 1523422)
- alternative location
- http://www.sciencedirect.com/science/article/B6T7J-4XY4GM3-1/2/940c9723db4e55f3381879994375ae51
- date added to LUP
- 2016-04-01 10:10:50
- date last changed
- 2022-03-27 05:40:57
@article{4a7ef840-6f9b-4820-9fde-09d15bf95bbb,
abstract = {{Decompaction of DNA-CTA self-assembled complexes by 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) was studied and the results were compared with beta-CD. Different degrees of 2-HP substitution (0.6, 0.8 and 1.0, respectively) were used and the decompaction was successful with all degrees of substitution. Fluorescence microscopy, steady state fluorescence spectroscopy, density and sound velocity measurements, thermal melting and circular dichroism were used. Compared to previous work using alpha- and beta-CD, the fluorescence spectroscopy results showed that the 2-HP-substituted CDs more efficiently released DNA into solution. Furthermore, dissociation of macroscopically phase separated DNA-CTA complexes was achieved upon addition of 2-HP-beta-CD and the results gave strong indications on the non-equilibrium nature of the system. The globule-to-coil transition was not found to proceed through a coexistence region, which seems to be a general phenomenon in DNA decompaction using CDs.}},
author = {{Carlstedt, Jonas and Gonzalez Perez, Alfredo and Alatorre Meda, Manuel and Dias, Rita and Lindman, Björn}},
issn = {{1879-0003}},
language = {{eng}},
number = {{2}},
pages = {{153--158}},
publisher = {{Elsevier}},
series = {{International Journal of Biological Macromolecules}},
title = {{Release of DNA from surfactant complexes induced by 2-hydroxypropyl-beta-cyclodextrin.}},
url = {{http://dx.doi.org/10.1016/j.ijbiomac.2009.12.002}},
doi = {{10.1016/j.ijbiomac.2009.12.002}},
volume = {{46}},
year = {{2010}},
}