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DNA Heats Up: Energetics of Genome Ejection from Phage Revealed by Isothermal Titration Calorimetry.

Jeembaeva, Meerim LU ; Jönsson, Bengt LU ; Castelnovo, Martin and Evilevitch, Alex LU (2010) In Journal of Molecular Biology 395(5). p.1079-1087
Abstract
Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion to the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now, using either single-molecule or osmotic suppression techniques. In this work, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or, equivalently, the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the... (More)
Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion to the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now, using either single-molecule or osmotic suppression techniques. In this work, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or, equivalently, the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the results lead to the identification of thermodynamic determinants associated with DNA ejection. The values obtained were found to be consistent with those previously predicted by analytical models and numerical simulations. Moreover, the results confirm the role of DNA hydration in the energetics of genome confinement in viral capsids. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Molecular Biology
volume
395
issue
5
pages
1079 - 1087
publisher
Elsevier
external identifiers
  • wos:000274922400015
  • pmid:19969001
  • scopus:73649136056
ISSN
1089-8638
DOI
10.1016/j.jmb.2009.11.069
language
English
LU publication?
yes
id
bcc7683d-27a9-44cc-bb09-92f7e2e6f1f9 (old id 1523830)
date added to LUP
2010-01-15 10:33:30
date last changed
2018-06-10 03:55:23
@article{bcc7683d-27a9-44cc-bb09-92f7e2e6f1f9,
  abstract     = {Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion to the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now, using either single-molecule or osmotic suppression techniques. In this work, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or, equivalently, the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the results lead to the identification of thermodynamic determinants associated with DNA ejection. The values obtained were found to be consistent with those previously predicted by analytical models and numerical simulations. Moreover, the results confirm the role of DNA hydration in the energetics of genome confinement in viral capsids.},
  author       = {Jeembaeva, Meerim and Jönsson, Bengt and Castelnovo, Martin and Evilevitch, Alex},
  issn         = {1089-8638},
  language     = {eng},
  number       = {5},
  pages        = {1079--1087},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Biology},
  title        = {DNA Heats Up: Energetics of Genome Ejection from Phage Revealed by Isothermal Titration Calorimetry.},
  url          = {http://dx.doi.org/10.1016/j.jmb.2009.11.069},
  volume       = {395},
  year         = {2010},
}