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Ectoine Production by Halomonas boliviensis: Optimization Using Response Surface Methodology.

Doan Van, Thuoc LU ; Guzmán, Hector LU ; Thi-Hang, Mai and Hatti-Kaul, Rajni LU (2010) In Marine Biotechnology 12. p.586-593
Abstract
Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH(4)Cl, K(2)HPO(4), and MgSO(4)*7H(2)O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good... (More)
Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH(4)Cl, K(2)HPO(4), and MgSO(4)*7H(2)O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good agreement with the predicted values. The optimized conditions were also used for two-step 1.5-L fed-batch fermentations. In the first step, biomass concentration of 28.7 g/L was obtained while in the second step biomass concentration increased to 63 g/L. Ectoine concentration of 9.2 g/L was obtained, and the overall ectoine productivity was 6.3 g/L/day, being among the highest reported so far. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Marine Biotechnology
volume
12
pages
586 - 593
publisher
Springer
external identifiers
  • wos:000282592900011
  • pmid:19957094
  • scopus:77957756253
ISSN
1436-2236
DOI
10.1007/s10126-009-9246-6
language
English
LU publication?
yes
id
ae468024-67a4-486d-bf0a-308dc527d43d (old id 1524065)
date added to LUP
2010-01-11 12:08:42
date last changed
2018-06-17 03:36:49
@article{ae468024-67a4-486d-bf0a-308dc527d43d,
  abstract     = {Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH(4)Cl, K(2)HPO(4), and MgSO(4)*7H(2)O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good agreement with the predicted values. The optimized conditions were also used for two-step 1.5-L fed-batch fermentations. In the first step, biomass concentration of 28.7 g/L was obtained while in the second step biomass concentration increased to 63 g/L. Ectoine concentration of 9.2 g/L was obtained, and the overall ectoine productivity was 6.3 g/L/day, being among the highest reported so far.},
  author       = {Doan Van, Thuoc and Guzmán, Hector and Thi-Hang, Mai and Hatti-Kaul, Rajni},
  issn         = {1436-2236},
  language     = {eng},
  pages        = {586--593},
  publisher    = {Springer},
  series       = {Marine Biotechnology},
  title        = {Ectoine Production by Halomonas boliviensis: Optimization Using Response Surface Methodology.},
  url          = {http://dx.doi.org/10.1007/s10126-009-9246-6},
  volume       = {12},
  year         = {2010},
}