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ELISA on a microchip with a photodiode for detection of amphetamine in plasma and urine

Mobini Far, Hamid Reza LU ; Torabi, F; Danielsson, Bengt LU and Khayyami, M (2005) In Journal of Analytical Toxicology 29(8). p.790-793
Abstract
A rapid and sensitive assay was developed for the detection of amphetamine in plasma and urine. The method relies on the principle of competitive ELISA (enzyme-linked immunosorbent assay). A flow microchip with a total volume of 7 µL was used for the development of a chemiluminescent ELISA technique. Solutions, samples, and the chemiluminescence substrate were injected by a flow system, and a photodiode detector was used to measure the light intensity. The incubation time of the competitor (competition phase) was reduced to 10 min. Calibration curves corresponding to analyte concentrations ranging from 40 to 1000 µg/L in urine samples and from 6 to 96 µg/L in plasma samples were obtained. The detection limits were in the region of 20 and 6... (More)
A rapid and sensitive assay was developed for the detection of amphetamine in plasma and urine. The method relies on the principle of competitive ELISA (enzyme-linked immunosorbent assay). A flow microchip with a total volume of 7 µL was used for the development of a chemiluminescent ELISA technique. Solutions, samples, and the chemiluminescence substrate were injected by a flow system, and a photodiode detector was used to measure the light intensity. The incubation time of the competitor (competition phase) was reduced to 10 min. Calibration curves corresponding to analyte concentrations ranging from 40 to 1000 µg/L in urine samples and from 6 to 96 µg/L in plasma samples were obtained. The detection limits were in the region of 20 and 6 µg/L in urine and plasma, respectively. The main focus of the work was on speed, reliability, reproducibility, and operational stability of the assay. This method was proven readily adaptable to automation and provided reproducible results. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Analytical Toxicology
volume
29
issue
8
pages
790 - 793
publisher
Preston Publications
external identifiers
  • wos:000233448800004
  • scopus:29144502366
ISSN
1945-2403
language
English
LU publication?
yes
id
24d97f51-9037-4620-a644-31244e67e960 (old id 152570)
alternative location
http://www.ingentaconnect.com/content/pres/jat/2005/00000029/00000008/art00004
date added to LUP
2007-07-16 09:09:48
date last changed
2017-03-19 04:14:52
@article{24d97f51-9037-4620-a644-31244e67e960,
  abstract     = {A rapid and sensitive assay was developed for the detection of amphetamine in plasma and urine. The method relies on the principle of competitive ELISA (enzyme-linked immunosorbent assay). A flow microchip with a total volume of 7 µL was used for the development of a chemiluminescent ELISA technique. Solutions, samples, and the chemiluminescence substrate were injected by a flow system, and a photodiode detector was used to measure the light intensity. The incubation time of the competitor (competition phase) was reduced to 10 min. Calibration curves corresponding to analyte concentrations ranging from 40 to 1000 µg/L in urine samples and from 6 to 96 µg/L in plasma samples were obtained. The detection limits were in the region of 20 and 6 µg/L in urine and plasma, respectively. The main focus of the work was on speed, reliability, reproducibility, and operational stability of the assay. This method was proven readily adaptable to automation and provided reproducible results.},
  author       = {Mobini Far, Hamid Reza and Torabi, F and Danielsson, Bengt and Khayyami, M},
  issn         = {1945-2403},
  language     = {eng},
  number       = {8},
  pages        = {790--793},
  publisher    = {Preston Publications},
  series       = {Journal of Analytical Toxicology},
  title        = {ELISA on a microchip with a photodiode for detection of amphetamine in plasma and urine},
  volume       = {29},
  year         = {2005},
}