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Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine

Hendrickson, O D; Wärnmark, Ioana LU ; Sitdikov, R; Zherdev, A V; Dzantiev, B B and Danielsson, Bengt LU (2005) In International Journal of Environmental Analytical Chemistry 85(12-13). p.905-915
Abstract
A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in... (More)
A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in the range 1-7%. The effectiveness of the assays developed for controlling triazine herbicides in mineral and tap water has been demonstrated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Environmental Analytical Chemistry
volume
85
issue
12-13
pages
905 - 915
publisher
Taylor & Francis
external identifiers
  • wos:000232033600010
  • scopus:33745063091
ISSN
1029-0397
DOI
10.1080/03067310500151144
language
English
LU publication?
yes
id
cca0d6a5-d787-4e47-bfdd-eab7ca361f83 (old id 152611)
date added to LUP
2007-07-16 09:43:01
date last changed
2017-01-01 04:40:03
@article{cca0d6a5-d787-4e47-bfdd-eab7ca361f83,
  abstract     = {A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in the range 1-7%. The effectiveness of the assays developed for controlling triazine herbicides in mineral and tap water has been demonstrated.},
  author       = {Hendrickson, O D and Wärnmark, Ioana and Sitdikov, R and Zherdev, A V and Dzantiev, B B and Danielsson, Bengt},
  issn         = {1029-0397},
  language     = {eng},
  number       = {12-13},
  pages        = {905--915},
  publisher    = {Taylor & Francis},
  series       = {International Journal of Environmental Analytical Chemistry},
  title        = {Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine},
  url          = {http://dx.doi.org/10.1080/03067310500151144},
  volume       = {85},
  year         = {2005},
}