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Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate format

Hanora, Amro LU ; Bernaudat, Florent LU ; Plieva, Fatima LU ; Dainiak, Maria LU ; Bülow, Leif LU ; Galaev, Igor LU and Mattiasson, Bo LU (2005) In Journal of Chromatography A 1087(1-2). p.38-44
Abstract
A method for high throughput screening of Green Fluorescent Proteins carrying metal binding tags in bacteria was developed. A random four amino acids tag-peptide library was successfully generated in E. coli. A 96-microtiter plate assembled with metal-iminodiacetic acid small cryogel columns was used for library screening. For the first time we were able to simultaneously screen a metal binding peptide tags library obtained from E. coli against different metal ions. From screening 25 different tags, three clones were able to bind to all metal ions studied (Ni2+, Zn2+, Co2+ and Cd2+). It was clearly demonstrated that the new construct could facilitate the screening of large peptide libraries. (c) 2005 Elsevier B.V. All rights reserved.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
1087
issue
1-2
pages
38 - 44
publisher
Elsevier
external identifiers
  • pmid:16130695
  • wos:000231185600006
  • scopus:23044450523
ISSN
0021-9673
DOI
10.1016/j.chroma.2005.04.029
language
English
LU publication?
yes
id
45b57889-4542-48ce-b986-66f108ee7c47 (old id 152619)
date added to LUP
2007-07-02 11:19:09
date last changed
2017-03-05 04:04:16
@article{45b57889-4542-48ce-b986-66f108ee7c47,
  abstract     = {A method for high throughput screening of Green Fluorescent Proteins carrying metal binding tags in bacteria was developed. A random four amino acids tag-peptide library was successfully generated in E. coli. A 96-microtiter plate assembled with metal-iminodiacetic acid small cryogel columns was used for library screening. For the first time we were able to simultaneously screen a metal binding peptide tags library obtained from E. coli against different metal ions. From screening 25 different tags, three clones were able to bind to all metal ions studied (Ni2+, Zn2+, Co2+ and Cd2+). It was clearly demonstrated that the new construct could facilitate the screening of large peptide libraries. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Hanora, Amro and Bernaudat, Florent and Plieva, Fatima and Dainiak, Maria and Bülow, Leif and Galaev, Igor and Mattiasson, Bo},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1-2},
  pages        = {38--44},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate format},
  url          = {http://dx.doi.org/10.1016/j.chroma.2005.04.029},
  volume       = {1087},
  year         = {2005},
}