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Selection of DNA aptamers against rat liver X receptors

Wärnmark, Ioana LU ; Wärnmark, Anette; Toresson, G; Gustafsson, J A and Bülow, Leif LU (2005) In Biochemical and Biophysical Research Communications 332(2). p.512-517
Abstract
Liver X receptors alpha and beta (LXRα; LXRβ) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors. LXRs play an important role in the reverse cholesterol transport and govern the expression of many of the proteins that are indispensable for the regulation of normal cholesterol levels in the body. SELEX, an in vitro selection technology, was used on a single stranded DNA library harboring a 12 randomized nucleotide sequence in order to isolate aptamers showing affinity for LXRα. Enzyme-linked assays and surface plasmon resonance measurements showed that the selected aptamers had strong affinities for LXRa with apparent dissociation constants, K(d)s, in nanomolar range. All clones carried... (More)
Liver X receptors alpha and beta (LXRα; LXRβ) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors. LXRs play an important role in the reverse cholesterol transport and govern the expression of many of the proteins that are indispensable for the regulation of normal cholesterol levels in the body. SELEX, an in vitro selection technology, was used on a single stranded DNA library harboring a 12 randomized nucleotide sequence in order to isolate aptamers showing affinity for LXRα. Enzyme-linked assays and surface plasmon resonance measurements showed that the selected aptamers had strong affinities for LXRa with apparent dissociation constants, K(d)s, in nanomolar range. All clones carried CG-repeats, indicating a probability for a similar manner of binding to LXRα. Very high cross-reactivities were observed when testing the aptamers with LXRβ (up to 700%) and RXRα (up to 50%). If instead we regard the aptamer sequences as selected against LXRβ, the cross-reactivities decrease considerably, to 17% for LXRα and 7% for RXRα. Therefore, in the future we are planning to use the obtained aptamers as binders for LXRβ. (C) 2005 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aptamer, SPR, LXR alpha, LXR beta, SELEX
in
Biochemical and Biophysical Research Communications
volume
332
issue
2
pages
512 - 517
publisher
Elsevier
external identifiers
  • wos:000229572300029
  • pmid:15910755
  • scopus:19444388227
ISSN
1090-2104
DOI
10.1016/j.bbrc.2005.04.147
language
English
LU publication?
yes
id
0df06640-ccd4-45dc-b0a4-b518c095d780 (old id 152633)
date added to LUP
2007-07-17 08:14:43
date last changed
2017-01-01 07:07:25
@article{0df06640-ccd4-45dc-b0a4-b518c095d780,
  abstract     = {Liver X receptors alpha and beta (LXRα; LXRβ) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors. LXRs play an important role in the reverse cholesterol transport and govern the expression of many of the proteins that are indispensable for the regulation of normal cholesterol levels in the body. SELEX, an in vitro selection technology, was used on a single stranded DNA library harboring a 12 randomized nucleotide sequence in order to isolate aptamers showing affinity for LXRα. Enzyme-linked assays and surface plasmon resonance measurements showed that the selected aptamers had strong affinities for LXRa with apparent dissociation constants, K(d)s, in nanomolar range. All clones carried CG-repeats, indicating a probability for a similar manner of binding to LXRα. Very high cross-reactivities were observed when testing the aptamers with LXRβ (up to 700%) and RXRα (up to 50%). If instead we regard the aptamer sequences as selected against LXRβ, the cross-reactivities decrease considerably, to 17% for LXRα and 7% for RXRα. Therefore, in the future we are planning to use the obtained aptamers as binders for LXRβ. (C) 2005 Elsevier Inc. All rights reserved.},
  author       = {Wärnmark, Ioana and Wärnmark, Anette and Toresson, G and Gustafsson, J A and Bülow, Leif},
  issn         = {1090-2104},
  keyword      = {aptamer,SPR,LXR alpha,LXR beta,SELEX},
  language     = {eng},
  number       = {2},
  pages        = {512--517},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Selection of DNA aptamers against rat liver X receptors},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2005.04.147},
  volume       = {332},
  year         = {2005},
}