Advanced

Organ bath in detecting the effect of one-hour warm ischemia on pulmonic arteries and bronchi from non-heart-beating donor lungs

Zhao, Song; Wang, Jia-xiang; Yang, Zhan-feng; He, Zhan-feng and Liao, Qiuming LU (2009) In Chinese Medical Journal 122(23). p.2903-2906
Abstract
Background Non-heart-beating donor lung has been a promising source of lung transplantation. Many studies on non-heart-beating donor lungs are based on animal lung transplantation. In this study, we assessed by organ bath the effect of one-hour warm ischemia on the non-heart-beating donor lung in terms of the integrity of contractile and relaxant functions and tissue structures of pulmonic arteries and bronchi. Methods Sixteen Swedish pigs were randomly classified into two groups: heart-beating donor group and 1-hour warm ischemia non-heart-beating donor group. Pulmonic and bronchial rings were taken from the isolated left lungs of the pigs. The pulmonic rings were stimulated by U-46619 (5.7 mol/L) and acetylcholine (10(-4) mmol/L) to... (More)
Background Non-heart-beating donor lung has been a promising source of lung transplantation. Many studies on non-heart-beating donor lungs are based on animal lung transplantation. In this study, we assessed by organ bath the effect of one-hour warm ischemia on the non-heart-beating donor lung in terms of the integrity of contractile and relaxant functions and tissue structures of pulmonic arteries and bronchi. Methods Sixteen Swedish pigs were randomly classified into two groups: heart-beating donor group and 1-hour warm ischemia non-heart-beating donor group. Pulmonic and bronchial rings were taken from the isolated left lungs of the pigs. The pulmonic rings were stimulated by U-46619 (5.7 mol/L) and acetylcholine (10(-4) mmol/L) to assess the contractile abilities of smooth muscle and the endothelium-dependent relaxation response, respectively. As such, acetylcholine (10(-5) mmol/L) and natrium arachidonic acid (0.01%) were used to detect the contraction of bronchial smooth muscle and epithelium-dependent relaxation response. Meanwhile, the variances of precontraction tension of control groups were recorded to measure whether there was spontaneous relaxation during endothelium/epithelium-dependent relaxation course. Finally, papaverine solution (10(-4) mmol/L) was used to detect the non-endothelium/epithelium-dependent relaxant abilities of pulmonic and bronchial smooth muscles. Results There was no significant difference in the tension values of precontraction of pulmonic rings (P >0.05), endothelium-dependent relaxation (P >0.05), precontraction of bronchial rings (P >0.05) and epithelium-dependent relaxation (P >0.05) between the heart-beating donor group and the 1-hour warm ischemia non-heart-beating donor group. And the pulmonic and bronchial rings of each subgroup B had no spontaneous relaxation. Finally, papaverine solution relaxed the smooth muscle of all the rings completely. Conclusions The results of this experiment suggest that the contractile and relaxant functions and tissue structures of pulmonic arteries and bronchi are not damaged after warm ischemia for 1 hour, and support the further study of ;non-heart-beating donor lung. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
epithelium-dependent relaxant, non-heart-beating donor, endothelium-dependent relaxation, organ bath
in
Chinese Medical Journal
volume
122
issue
23
pages
2903 - 2906
publisher
Chinese Medical Association
external identifiers
  • wos:000272598800019
  • scopus:74549153250
ISSN
0366-6999
DOI
10.3760/cma.j.issn.0366-6999.2009.23.019
language
English
LU publication?
yes
id
96775cda-bdb7-47e9-bd10-5ccfb95351cc (old id 1532745)
date added to LUP
2010-01-29 09:08:53
date last changed
2017-01-01 06:31:35
@article{96775cda-bdb7-47e9-bd10-5ccfb95351cc,
  abstract     = {Background Non-heart-beating donor lung has been a promising source of lung transplantation. Many studies on non-heart-beating donor lungs are based on animal lung transplantation. In this study, we assessed by organ bath the effect of one-hour warm ischemia on the non-heart-beating donor lung in terms of the integrity of contractile and relaxant functions and tissue structures of pulmonic arteries and bronchi. Methods Sixteen Swedish pigs were randomly classified into two groups: heart-beating donor group and 1-hour warm ischemia non-heart-beating donor group. Pulmonic and bronchial rings were taken from the isolated left lungs of the pigs. The pulmonic rings were stimulated by U-46619 (5.7 mol/L) and acetylcholine (10(-4) mmol/L) to assess the contractile abilities of smooth muscle and the endothelium-dependent relaxation response, respectively. As such, acetylcholine (10(-5) mmol/L) and natrium arachidonic acid (0.01%) were used to detect the contraction of bronchial smooth muscle and epithelium-dependent relaxation response. Meanwhile, the variances of precontraction tension of control groups were recorded to measure whether there was spontaneous relaxation during endothelium/epithelium-dependent relaxation course. Finally, papaverine solution (10(-4) mmol/L) was used to detect the non-endothelium/epithelium-dependent relaxant abilities of pulmonic and bronchial smooth muscles. Results There was no significant difference in the tension values of precontraction of pulmonic rings (P >0.05), endothelium-dependent relaxation (P >0.05), precontraction of bronchial rings (P >0.05) and epithelium-dependent relaxation (P >0.05) between the heart-beating donor group and the 1-hour warm ischemia non-heart-beating donor group. And the pulmonic and bronchial rings of each subgroup B had no spontaneous relaxation. Finally, papaverine solution relaxed the smooth muscle of all the rings completely. Conclusions The results of this experiment suggest that the contractile and relaxant functions and tissue structures of pulmonic arteries and bronchi are not damaged after warm ischemia for 1 hour, and support the further study of ;non-heart-beating donor lung.},
  author       = {Zhao, Song and Wang, Jia-xiang and Yang, Zhan-feng and He, Zhan-feng and Liao, Qiuming},
  issn         = {0366-6999},
  keyword      = {epithelium-dependent relaxant,non-heart-beating donor,endothelium-dependent relaxation,organ bath},
  language     = {eng},
  number       = {23},
  pages        = {2903--2906},
  publisher    = {Chinese Medical Association},
  series       = {Chinese Medical Journal},
  title        = {Organ bath in detecting the effect of one-hour warm ischemia on pulmonic arteries and bronchi from non-heart-beating donor lungs},
  url          = {http://dx.doi.org/10.3760/cma.j.issn.0366-6999.2009.23.019},
  volume       = {122},
  year         = {2009},
}