Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Development of a non-destructive micro-analytical method for stable carbon isotope analysis of transmission electron microscope (TEM) samples

Hode, Tomas ; Kristiansson, Per LU ; Elfman, Mikael LU ; Hugo, Richard C. and Cady, Sherry L. (2009) In Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms 267(19). p.3375-3382
Abstract
The biogenicity of ancient morphological microfossil-like objects can be established by linking morphological (e.g. cell remnants and extracellular polymeric matrix) and chemical (e.g. isotopes, biomarkers and biominerals) evidence indicative of microorganisms or microbial activity. We have developed a non-destructive micro-analytical ion beam system capable of measuring with high spatial resolution the stable carbon isotope ratios of thin samples used for transmission electron microscopy. The technique is based on elastic scattering of alpha particles with an energy of 2.751 MeV. At this energy the C-13 cross section is enhanced relative to the pure Rutherford cross section for C-13, whereas the C-12 cross section is reduced relative to... (More)
The biogenicity of ancient morphological microfossil-like objects can be established by linking morphological (e.g. cell remnants and extracellular polymeric matrix) and chemical (e.g. isotopes, biomarkers and biominerals) evidence indicative of microorganisms or microbial activity. We have developed a non-destructive micro-analytical ion beam system capable of measuring with high spatial resolution the stable carbon isotope ratios of thin samples used for transmission electron microscopy. The technique is based on elastic scattering of alpha particles with an energy of 2.751 MeV. At this energy the C-13 cross section is enhanced relative to the pure Rutherford cross section for C-13, whereas the C-12 cross section is reduced relative to its pure Rutherford cross section. Here we report the initial results of this experimental approach used to characterize ultramicrotomed sections of sulfur-embedded graphite and microbial cells. (C) 2009 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Carbon isotopes, Astrobiology, Rutherford scattering, TEM
in
Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
volume
267
issue
19
pages
3375 - 3382
publisher
Elsevier
external identifiers
  • wos:000272422800014
  • scopus:70149095238
ISSN
0168-583X
DOI
10.1016/j.nimb.2009.06.116
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007)
id
72fda413-4fff-402a-9740-b99ae6887e26 (old id 1533408)
date added to LUP
2016-04-01 13:47:36
date last changed
2021-06-08 04:20:58
@article{72fda413-4fff-402a-9740-b99ae6887e26,
  abstract     = {The biogenicity of ancient morphological microfossil-like objects can be established by linking morphological (e.g. cell remnants and extracellular polymeric matrix) and chemical (e.g. isotopes, biomarkers and biominerals) evidence indicative of microorganisms or microbial activity. We have developed a non-destructive micro-analytical ion beam system capable of measuring with high spatial resolution the stable carbon isotope ratios of thin samples used for transmission electron microscopy. The technique is based on elastic scattering of alpha particles with an energy of 2.751 MeV. At this energy the C-13 cross section is enhanced relative to the pure Rutherford cross section for C-13, whereas the C-12 cross section is reduced relative to its pure Rutherford cross section. Here we report the initial results of this experimental approach used to characterize ultramicrotomed sections of sulfur-embedded graphite and microbial cells. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Hode, Tomas and Kristiansson, Per and Elfman, Mikael and Hugo, Richard C. and Cady, Sherry L.},
  issn         = {0168-583X},
  language     = {eng},
  number       = {19},
  pages        = {3375--3382},
  publisher    = {Elsevier},
  series       = {Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms},
  title        = {Development of a non-destructive micro-analytical method for stable carbon isotope analysis of transmission electron microscope (TEM) samples},
  url          = {http://dx.doi.org/10.1016/j.nimb.2009.06.116},
  doi          = {10.1016/j.nimb.2009.06.116},
  volume       = {267},
  year         = {2009},
}