Role of Linker Groups between Hydrophilic and Hydrophobic Moieties of Cationic Surfactants on Oligonucleotide-Surfactant Interactions
(2009) In Langmuir 25(24). p.13770-13775- Abstract
- The interaction between DNA and amino-acid-based surfactants with different linker groups was investigated by gel electrophoresis, ethidium bromide exclusion assays, circular dichroism, and melting temperature determinations. The Studies showed that the strength of the interaction between the oligonucleotides and the surfactants is highly dependent oil the linker of the surfactant. For ester surfactants, no significant interaction was observed for surfactant-to-DNA charge ratios tip to 12. On the other hand, amide surfactants were shown to interact strongly with the oligonucleotides; these surfactants could displace up to 75% of the ethidium bromide molecules bound to the DNA and induced significant changes in the circular dichroism... (More)
- The interaction between DNA and amino-acid-based surfactants with different linker groups was investigated by gel electrophoresis, ethidium bromide exclusion assays, circular dichroism, and melting temperature determinations. The Studies showed that the strength of the interaction between the oligonucleotides and the surfactants is highly dependent oil the linker of the surfactant. For ester surfactants, no significant interaction was observed for surfactant-to-DNA charge ratios tip to 12. On the other hand, amide surfactants were shown to interact strongly with the oligonucleotides; these surfactants could displace up to 75% of the ethidium bromide molecules bound to the DNA and induced significant changes in the circular dichroism spectra. When comparing the headgroups of the surfactants, it was observed that surfactants with more hydrophobic headgroups (proline vs alanine) interacted more strongly With the DNA, in good agreement With previous studies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1533422
- author
- Santhiya, Deenan ; Dias, Rita S. ; Shome, Anshupriya ; Das, Prasanta Kumar ; Miguel, Maria G. ; Lindman, Björn LU and Maiti, Souvik
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 25
- issue
- 24
- pages
- 13770 - 13775
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000272394400011
- scopus:74049084168
- ISSN
- 0743-7463
- DOI
- 10.1021/la901546t
- language
- English
- LU publication?
- yes
- id
- 9c0cb66b-5112-4a50-97e6-28a526cc5d18 (old id 1533422)
- date added to LUP
- 2016-04-01 12:15:59
- date last changed
- 2022-01-27 01:12:02
@article{9c0cb66b-5112-4a50-97e6-28a526cc5d18, abstract = {{The interaction between DNA and amino-acid-based surfactants with different linker groups was investigated by gel electrophoresis, ethidium bromide exclusion assays, circular dichroism, and melting temperature determinations. The Studies showed that the strength of the interaction between the oligonucleotides and the surfactants is highly dependent oil the linker of the surfactant. For ester surfactants, no significant interaction was observed for surfactant-to-DNA charge ratios tip to 12. On the other hand, amide surfactants were shown to interact strongly with the oligonucleotides; these surfactants could displace up to 75% of the ethidium bromide molecules bound to the DNA and induced significant changes in the circular dichroism spectra. When comparing the headgroups of the surfactants, it was observed that surfactants with more hydrophobic headgroups (proline vs alanine) interacted more strongly With the DNA, in good agreement With previous studies.}}, author = {{Santhiya, Deenan and Dias, Rita S. and Shome, Anshupriya and Das, Prasanta Kumar and Miguel, Maria G. and Lindman, Björn and Maiti, Souvik}}, issn = {{0743-7463}}, language = {{eng}}, number = {{24}}, pages = {{13770--13775}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Langmuir}}, title = {{Role of Linker Groups between Hydrophilic and Hydrophobic Moieties of Cationic Surfactants on Oligonucleotide-Surfactant Interactions}}, url = {{http://dx.doi.org/10.1021/la901546t}}, doi = {{10.1021/la901546t}}, volume = {{25}}, year = {{2009}}, }