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Role of Linker Groups between Hydrophilic and Hydrophobic Moieties of Cationic Surfactants on Oligonucleotide-Surfactant Interactions

Santhiya, Deenan; Dias, Rita S.; Shome, Anshupriya; Das, Prasanta Kumar; Miguel, Maria G.; Lindman, Björn LU and Maiti, Souvik (2009) In Langmuir 25(24). p.13770-13775
Abstract
The interaction between DNA and amino-acid-based surfactants with different linker groups was investigated by gel electrophoresis, ethidium bromide exclusion assays, circular dichroism, and melting temperature determinations. The Studies showed that the strength of the interaction between the oligonucleotides and the surfactants is highly dependent oil the linker of the surfactant. For ester surfactants, no significant interaction was observed for surfactant-to-DNA charge ratios tip to 12. On the other hand, amide surfactants were shown to interact strongly with the oligonucleotides; these surfactants could displace up to 75% of the ethidium bromide molecules bound to the DNA and induced significant changes in the circular dichroism... (More)
The interaction between DNA and amino-acid-based surfactants with different linker groups was investigated by gel electrophoresis, ethidium bromide exclusion assays, circular dichroism, and melting temperature determinations. The Studies showed that the strength of the interaction between the oligonucleotides and the surfactants is highly dependent oil the linker of the surfactant. For ester surfactants, no significant interaction was observed for surfactant-to-DNA charge ratios tip to 12. On the other hand, amide surfactants were shown to interact strongly with the oligonucleotides; these surfactants could displace up to 75% of the ethidium bromide molecules bound to the DNA and induced significant changes in the circular dichroism spectra. When comparing the headgroups of the surfactants, it was observed that surfactants with more hydrophobic headgroups (proline vs alanine) interacted more strongly With the DNA, in good agreement With previous studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
25
issue
24
pages
13770 - 13775
publisher
The American Chemical Society
external identifiers
  • wos:000272394400011
  • scopus:74049084168
ISSN
0743-7463
DOI
10.1021/la901546t
language
English
LU publication?
yes
id
9c0cb66b-5112-4a50-97e6-28a526cc5d18 (old id 1533422)
date added to LUP
2010-01-28 16:07:12
date last changed
2017-01-01 04:59:26
@article{9c0cb66b-5112-4a50-97e6-28a526cc5d18,
  abstract     = {The interaction between DNA and amino-acid-based surfactants with different linker groups was investigated by gel electrophoresis, ethidium bromide exclusion assays, circular dichroism, and melting temperature determinations. The Studies showed that the strength of the interaction between the oligonucleotides and the surfactants is highly dependent oil the linker of the surfactant. For ester surfactants, no significant interaction was observed for surfactant-to-DNA charge ratios tip to 12. On the other hand, amide surfactants were shown to interact strongly with the oligonucleotides; these surfactants could displace up to 75% of the ethidium bromide molecules bound to the DNA and induced significant changes in the circular dichroism spectra. When comparing the headgroups of the surfactants, it was observed that surfactants with more hydrophobic headgroups (proline vs alanine) interacted more strongly With the DNA, in good agreement With previous studies.},
  author       = {Santhiya, Deenan and Dias, Rita S. and Shome, Anshupriya and Das, Prasanta Kumar and Miguel, Maria G. and Lindman, Björn and Maiti, Souvik},
  issn         = {0743-7463},
  language     = {eng},
  number       = {24},
  pages        = {13770--13775},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Role of Linker Groups between Hydrophilic and Hydrophobic Moieties of Cationic Surfactants on Oligonucleotide-Surfactant Interactions},
  url          = {http://dx.doi.org/10.1021/la901546t},
  volume       = {25},
  year         = {2009},
}