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A quantitative analysis of hydrogen production efficiency of the extreme thermophile Caldicellulosiruptor owensensis OL

Zeidan, Ahmad LU and van Niel, Ed LU (2010) In International Journal of Hydrogen Energy 35(3). p.1128-1137
Abstract
Caldicellulosiruptor owensensis strain OLT (DSM 13100) is an obligately anaerobic, extreme

thermophilic bacterium that is capable of utilizing a broad range of carbohydrates and

producing H2 as a metabolic by-product. The performance of C. owensensis on glucose and

xylose was analyzed in lab-scale bioreactors to assess its potential use in biohydrogen

production. Acetate, H2, and CO2 were the main end products during exponential growth of

the organism on either sugar. Lactate production was triggered during the transition into

the stationary phase and was associated with an increase in the levels of specific L-lactate

dehydrogenase activity. In addition, minor amounts of... (More)
Caldicellulosiruptor owensensis strain OLT (DSM 13100) is an obligately anaerobic, extreme

thermophilic bacterium that is capable of utilizing a broad range of carbohydrates and

producing H2 as a metabolic by-product. The performance of C. owensensis on glucose and

xylose was analyzed in lab-scale bioreactors to assess its potential use in biohydrogen

production. Acetate, H2, and CO2 were the main end products during exponential growth of

the organism on either sugar. Lactate production was triggered during the transition into

the stationary phase and was associated with an increase in the levels of specific L-lactate

dehydrogenase activity. In addition, minor amounts of ethanol and propionate could be

detected. H2 and acetate yields were lower on xylose than on glucose, marking an opposite

trend to biomass and lactate yields. The influence of elevated H2 partial pressure on

product distribution was more dramatic in xylose-fermenting cultures. Replacement of

yeast extract in the medium with a standard vitamins solution improved H2 yield on both

sugars, where it reached 100% of the theoretical maximum, i.e. 4 mol per mol hexose, on

glucose. By using the defined medium, both the maximum specific growth rate and the

maximum volumetric H2 production rate of C. owensensis increased significantly on glucose

and almost doubled on xylose. Screening other sugars besides glucose and xylose revealed

a clear sugar-dependent product-distribution pattern and a direct correlation between

biomass and lactate yields, which might be explained considering energy metabolism

of the cells. The organism is proposed as a new candidate for biohydrogen production at

high yields. (Less)
Please use this url to cite or link to this publication:
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organization
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type
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publication status
published
subject
keywords
Biohydrogen Extreme thermophile Caldicellulosiruptor owensensis Batch mode H2 partial pressure Lactate formation Defined medium
in
International Journal of Hydrogen Energy
volume
35
issue
3
pages
1128 - 1137
publisher
Elsevier
external identifiers
  • wos:000274944000029
  • scopus:74849131313
ISSN
1879-3487
DOI
10.1016/j.ijhydene.2009.11.082
language
English
LU publication?
yes
id
d6d691f8-483c-4903-ae49-b324a71c6c47 (old id 1540331)
date added to LUP
2010-02-04 15:00:08
date last changed
2018-07-08 03:48:04
@article{d6d691f8-483c-4903-ae49-b324a71c6c47,
  abstract     = {Caldicellulosiruptor owensensis strain OLT (DSM 13100) is an obligately anaerobic, extreme<br/><br>
thermophilic bacterium that is capable of utilizing a broad range of carbohydrates and<br/><br>
producing H2 as a metabolic by-product. The performance of C. owensensis on glucose and<br/><br>
xylose was analyzed in lab-scale bioreactors to assess its potential use in biohydrogen<br/><br>
production. Acetate, H2, and CO2 were the main end products during exponential growth of<br/><br>
the organism on either sugar. Lactate production was triggered during the transition into<br/><br>
the stationary phase and was associated with an increase in the levels of specific L-lactate<br/><br>
dehydrogenase activity. In addition, minor amounts of ethanol and propionate could be<br/><br>
detected. H2 and acetate yields were lower on xylose than on glucose, marking an opposite<br/><br>
trend to biomass and lactate yields. The influence of elevated H2 partial pressure on<br/><br>
product distribution was more dramatic in xylose-fermenting cultures. Replacement of<br/><br>
yeast extract in the medium with a standard vitamins solution improved H2 yield on both<br/><br>
sugars, where it reached 100% of the theoretical maximum, i.e. 4 mol per mol hexose, on<br/><br>
glucose. By using the defined medium, both the maximum specific growth rate and the<br/><br>
maximum volumetric H2 production rate of C. owensensis increased significantly on glucose<br/><br>
and almost doubled on xylose. Screening other sugars besides glucose and xylose revealed<br/><br>
a clear sugar-dependent product-distribution pattern and a direct correlation between<br/><br>
biomass and lactate yields, which might be explained considering energy metabolism<br/><br>
of the cells. The organism is proposed as a new candidate for biohydrogen production at<br/><br>
high yields.},
  author       = {Zeidan, Ahmad and van Niel, Ed},
  issn         = {1879-3487},
  keyword      = {Biohydrogen
Extreme thermophile
Caldicellulosiruptor owensensis
Batch mode
H2 partial pressure
Lactate formation
Defined medium},
  language     = {eng},
  number       = {3},
  pages        = {1128--1137},
  publisher    = {Elsevier},
  series       = {International Journal of Hydrogen Energy},
  title        = {A quantitative analysis of hydrogen production efficiency of the extreme thermophile Caldicellulosiruptor owensensis OL},
  url          = {http://dx.doi.org/10.1016/j.ijhydene.2009.11.082},
  volume       = {35},
  year         = {2010},
}