Advanced

A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nanogold colloids assembled on a polytyramine-modified gold electrode.

Labib, Mahmoud LU ; Hedström, Martin LU ; Amin, Magdy and Mattiasson, Bo LU (2010) In Analytica Chimica Acta 659(1-2). p.194-200
Abstract
A highly sensitive competitive capacitive glucose biosensor was constructed based on gold nanoparticles, which were employed as a platform to immobilize concanavalin A (Con A). Gold nanoparticles were fixed on a gold electrode, on which a layer of polytyramine was preformed via electrochemical polymerization. The sensing mechanism is based on the competitive dissociation of a glucose polymer or a glycoconjugate from the glycoligand binding sites of immobilized Con A by the added glucose. To further improve the sensor response, several glucose polymers as well as a synthesized glycoconjugate using the periodate method, were screened. Consequently, dextran (MW 39 kDa) was selected and the feasibility of the proposed biosensor was evaluated... (More)
A highly sensitive competitive capacitive glucose biosensor was constructed based on gold nanoparticles, which were employed as a platform to immobilize concanavalin A (Con A). Gold nanoparticles were fixed on a gold electrode, on which a layer of polytyramine was preformed via electrochemical polymerization. The sensing mechanism is based on the competitive dissociation of a glucose polymer or a glycoconjugate from the glycoligand binding sites of immobilized Con A by the added glucose. To further improve the sensor response, several glucose polymers as well as a synthesized glycoconjugate using the periodate method, were screened. Consequently, dextran (MW 39 kDa) was selected and the feasibility of the proposed biosensor was evaluated for a competitive assay of glucose. Experimental results show that the biosensor responded linearly to glucose in the range from 1.0 x 10(-6) to 1.0 x 10(-2) M, corresponding to 0.18 microg mL(-1) to 1.8 mg mL(-1) of glucose with a detection limit of 1.0 x 10(-6) M under optimized conditions. The studied biosensor exhibited a response time of about 15 min and a neglectable loss in sensitivity after 10 repeated analytical cycles. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
659
issue
1-2
pages
194 - 200
publisher
Elsevier
external identifiers
  • wos:000274046900025
  • pmid:20103124
  • scopus:72549098660
ISSN
1873-4324
DOI
10.1016/j.aca.2009.11.028
language
English
LU publication?
yes
id
5d0542f9-e8ef-429c-95e2-f8cb0e2df036 (old id 1540575)
date added to LUP
2010-02-10 09:57:41
date last changed
2018-05-29 09:23:32
@article{5d0542f9-e8ef-429c-95e2-f8cb0e2df036,
  abstract     = {A highly sensitive competitive capacitive glucose biosensor was constructed based on gold nanoparticles, which were employed as a platform to immobilize concanavalin A (Con A). Gold nanoparticles were fixed on a gold electrode, on which a layer of polytyramine was preformed via electrochemical polymerization. The sensing mechanism is based on the competitive dissociation of a glucose polymer or a glycoconjugate from the glycoligand binding sites of immobilized Con A by the added glucose. To further improve the sensor response, several glucose polymers as well as a synthesized glycoconjugate using the periodate method, were screened. Consequently, dextran (MW 39 kDa) was selected and the feasibility of the proposed biosensor was evaluated for a competitive assay of glucose. Experimental results show that the biosensor responded linearly to glucose in the range from 1.0 x 10(-6) to 1.0 x 10(-2) M, corresponding to 0.18 microg mL(-1) to 1.8 mg mL(-1) of glucose with a detection limit of 1.0 x 10(-6) M under optimized conditions. The studied biosensor exhibited a response time of about 15 min and a neglectable loss in sensitivity after 10 repeated analytical cycles.},
  author       = {Labib, Mahmoud and Hedström, Martin and Amin, Magdy and Mattiasson, Bo},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1-2},
  pages        = {194--200},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nanogold colloids assembled on a polytyramine-modified gold electrode.},
  url          = {http://dx.doi.org/10.1016/j.aca.2009.11.028},
  volume       = {659},
  year         = {2010},
}