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Increasing the coulombic efficiency of glucose biofuel cell anodes by combination of redox enzymes

Tasca, Federico LU ; Gorton, Lo LU ; Kujawa, Magdalena; Patel, Ilabahen; Harreither, Wolfgang; Peterbauer, Clemens K; Ludwig, Roland and Nöll, Gilbert LU (2010) In Biosensors & Bioelectronics 25. p.1710-1716
Abstract
A highly efficient anode for glucose biofuel cells has been developed by a combination of pyranose dehydrogenase from Agaricus meleagris (AmPDH) and cellobiose dehydrogenase from Myriococcum thermophilum (MtCDH). These two enzymes differ in how they oxidize glucose. AmPDH oxidizes glucose at the C(2) and C(3) carbon, whereas MtCDH at the C(1) carbon. Both enzymes oxidize efficiently a number of other mono- and disaccharides. They do not react directly with oxygen and produce no H(2)O(2). Electrodes were prepared by embedding (i) only AmPDH (in order to study this enzyme separately) and (ii) a mixture of AmPDH and MtCDH in an Os redox polymer hydrogel. Single-walled carbon nanotubes (SWCNTs) were added in order to enhance the current... (More)
A highly efficient anode for glucose biofuel cells has been developed by a combination of pyranose dehydrogenase from Agaricus meleagris (AmPDH) and cellobiose dehydrogenase from Myriococcum thermophilum (MtCDH). These two enzymes differ in how they oxidize glucose. AmPDH oxidizes glucose at the C(2) and C(3) carbon, whereas MtCDH at the C(1) carbon. Both enzymes oxidize efficiently a number of other mono- and disaccharides. They do not react directly with oxygen and produce no H(2)O(2). Electrodes were prepared by embedding (i) only AmPDH (in order to study this enzyme separately) and (ii) a mixture of AmPDH and MtCDH in an Os redox polymer hydrogel. Single-walled carbon nanotubes (SWCNTs) were added in order to enhance the current density. The electrodes were investigated with linear sweep and cyclic voltammetry in the presence of different substrates at physiological conditions. The electrochemical measurements revealed that the product of one enzyme can serve as a substrate for the other. In addition, a kinetic pathway analysis was performed by spectrophotometric measurements leading to the conclusion that up to six electrons can be gained from one glucose molecule through a combination of AmPDH and MtCDH. Hence, the combination of redox enzymes can lead to an enzymatic biofuel cell anode with an increased coulombic efficiency far beyond the usual yields of two electrons per substrate molecule. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biosensors & Bioelectronics
volume
25
pages
1710 - 1716
publisher
Elsevier
external identifiers
  • wos:000275978700026
  • pmid:20071159
  • scopus:77952298424
ISSN
1873-4235
DOI
10.1016/j.bios.2009.12.017
language
English
LU publication?
yes
id
c72e5054-2cff-4265-95fb-6c1d79c92ca7 (old id 1541109)
date added to LUP
2010-02-02 14:42:44
date last changed
2018-05-29 11:31:16
@article{c72e5054-2cff-4265-95fb-6c1d79c92ca7,
  abstract     = {A highly efficient anode for glucose biofuel cells has been developed by a combination of pyranose dehydrogenase from Agaricus meleagris (AmPDH) and cellobiose dehydrogenase from Myriococcum thermophilum (MtCDH). These two enzymes differ in how they oxidize glucose. AmPDH oxidizes glucose at the C(2) and C(3) carbon, whereas MtCDH at the C(1) carbon. Both enzymes oxidize efficiently a number of other mono- and disaccharides. They do not react directly with oxygen and produce no H(2)O(2). Electrodes were prepared by embedding (i) only AmPDH (in order to study this enzyme separately) and (ii) a mixture of AmPDH and MtCDH in an Os redox polymer hydrogel. Single-walled carbon nanotubes (SWCNTs) were added in order to enhance the current density. The electrodes were investigated with linear sweep and cyclic voltammetry in the presence of different substrates at physiological conditions. The electrochemical measurements revealed that the product of one enzyme can serve as a substrate for the other. In addition, a kinetic pathway analysis was performed by spectrophotometric measurements leading to the conclusion that up to six electrons can be gained from one glucose molecule through a combination of AmPDH and MtCDH. Hence, the combination of redox enzymes can lead to an enzymatic biofuel cell anode with an increased coulombic efficiency far beyond the usual yields of two electrons per substrate molecule.},
  author       = {Tasca, Federico and Gorton, Lo and Kujawa, Magdalena and Patel, Ilabahen and Harreither, Wolfgang and Peterbauer, Clemens K and Ludwig, Roland and Nöll, Gilbert},
  issn         = {1873-4235},
  language     = {eng},
  pages        = {1710--1716},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Increasing the coulombic efficiency of glucose biofuel cell anodes by combination of redox enzymes},
  url          = {http://dx.doi.org/10.1016/j.bios.2009.12.017},
  volume       = {25},
  year         = {2010},
}