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Failure of transdifferentiation of adult hematopoietic stem cells into neurons.

Roybon, Laurent LU ; Ma, Zhi LU ; Asztely, Fredrik ; Fosum, Anna ; Jacobsen, Sten Eirik W LU ; Brundin, Patrik LU and Li, Jia-Yi LU (2006) In Stem Cells 24(6). p.1594-1604
Abstract
Previous studies of bone marrow-derived stem cell transdifferentiation into neurons have not involved purified cell populations and determined their exact phenotype prior to differentiation. The present study investigates whether highly purified mouse adult hematopoietic stem cells (HSCs), characterized by lineage marker depletion and expression of the cell surface markers Sca1 and c-Kit (Lin(-)Sca1(+) c-Kit(+) [LSK]), can be stimulated to adopt a neuronal fate. When the HSCLSK cells were cultured in vitro in neuronal differentiation medium supplemented with retinoic acid, 50% of the cells expressed the neural progenitor marker nestin and no cells had become postmitotic. Electrophysiological recordings on neuron-like cells showed that... (More)
Previous studies of bone marrow-derived stem cell transdifferentiation into neurons have not involved purified cell populations and determined their exact phenotype prior to differentiation. The present study investigates whether highly purified mouse adult hematopoietic stem cells (HSCs), characterized by lineage marker depletion and expression of the cell surface markers Sca1 and c-Kit (Lin(-)Sca1(+) c-Kit(+) [LSK]), can be stimulated to adopt a neuronal fate. When the HSCLSK cells were cultured in vitro in neuronal differentiation medium supplemented with retinoic acid, 50% of the cells expressed the neural progenitor marker nestin and no cells had become postmitotic. Electrophysiological recordings on neuron-like cells showed that these cells were incapable of generating action potentials. When the HSCLSK cells either were grown in vitro together with neural precursor cells or were transplanted into the striatum or cerebellum of wild-type mouse, they either differentiated into Iba1-immunopositive macrophage/microglia or died. In conclusion, we demonstrate that adult HSCLSK cells do not have the capacity to leave the hematopoietic lineage and differentiate into neurons. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fluorescence-activated cell sorting, microglia macrophage, transdifferentiation, neural stem cells, plasticity, adult hematopoietic stem cells
in
Stem Cells
volume
24
issue
6
pages
1594 - 1604
publisher
AlphaMed Press
external identifiers
  • wos:000240639300024
  • pmid:16556707
  • scopus:33747589401
ISSN
1549-4918
DOI
10.1634/stemcells.2005-0548
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Stem Cell Center (013041110)
id
2fe52bd5-d3f6-44aa-b781-393d1662798b (old id 154425)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16556707&dopt=Abstract
date added to LUP
2016-04-01 15:38:04
date last changed
2021-09-29 04:32:17
@article{2fe52bd5-d3f6-44aa-b781-393d1662798b,
  abstract     = {Previous studies of bone marrow-derived stem cell transdifferentiation into neurons have not involved purified cell populations and determined their exact phenotype prior to differentiation. The present study investigates whether highly purified mouse adult hematopoietic stem cells (HSCs), characterized by lineage marker depletion and expression of the cell surface markers Sca1 and c-Kit (Lin(-)Sca1(+) c-Kit(+) [LSK]), can be stimulated to adopt a neuronal fate. When the HSCLSK cells were cultured in vitro in neuronal differentiation medium supplemented with retinoic acid, 50% of the cells expressed the neural progenitor marker nestin and no cells had become postmitotic. Electrophysiological recordings on neuron-like cells showed that these cells were incapable of generating action potentials. When the HSCLSK cells either were grown in vitro together with neural precursor cells or were transplanted into the striatum or cerebellum of wild-type mouse, they either differentiated into Iba1-immunopositive macrophage/microglia or died. In conclusion, we demonstrate that adult HSCLSK cells do not have the capacity to leave the hematopoietic lineage and differentiate into neurons.},
  author       = {Roybon, Laurent and Ma, Zhi and Asztely, Fredrik and Fosum, Anna and Jacobsen, Sten Eirik W and Brundin, Patrik and Li, Jia-Yi},
  issn         = {1549-4918},
  language     = {eng},
  number       = {6},
  pages        = {1594--1604},
  publisher    = {AlphaMed Press},
  series       = {Stem Cells},
  title        = {Failure of transdifferentiation of adult hematopoietic stem cells into neurons.},
  url          = {http://dx.doi.org/10.1634/stemcells.2005-0548},
  doi          = {10.1634/stemcells.2005-0548},
  volume       = {24},
  year         = {2006},
}