Failure of transdifferentiation of adult hematopoietic stem cells into neurons.
(2006) In Stem Cells 24(6). p.1594-1604- Abstract
- Previous studies of bone marrow-derived stem cell transdifferentiation into neurons have not involved purified cell populations and determined their exact phenotype prior to differentiation. The present study investigates whether highly purified mouse adult hematopoietic stem cells (HSCs), characterized by lineage marker depletion and expression of the cell surface markers Sca1 and c-Kit (Lin(-)Sca1(+) c-Kit(+) [LSK]), can be stimulated to adopt a neuronal fate. When the HSCLSK cells were cultured in vitro in neuronal differentiation medium supplemented with retinoic acid, 50% of the cells expressed the neural progenitor marker nestin and no cells had become postmitotic. Electrophysiological recordings on neuron-like cells showed that... (More)
- Previous studies of bone marrow-derived stem cell transdifferentiation into neurons have not involved purified cell populations and determined their exact phenotype prior to differentiation. The present study investigates whether highly purified mouse adult hematopoietic stem cells (HSCs), characterized by lineage marker depletion and expression of the cell surface markers Sca1 and c-Kit (Lin(-)Sca1(+) c-Kit(+) [LSK]), can be stimulated to adopt a neuronal fate. When the HSCLSK cells were cultured in vitro in neuronal differentiation medium supplemented with retinoic acid, 50% of the cells expressed the neural progenitor marker nestin and no cells had become postmitotic. Electrophysiological recordings on neuron-like cells showed that these cells were incapable of generating action potentials. When the HSCLSK cells either were grown in vitro together with neural precursor cells or were transplanted into the striatum or cerebellum of wild-type mouse, they either differentiated into Iba1-immunopositive macrophage/microglia or died. In conclusion, we demonstrate that adult HSCLSK cells do not have the capacity to leave the hematopoietic lineage and differentiate into neurons. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/154425
- author
- Roybon, Laurent LU ; Ma, Zhi LU ; Asztely, Fredrik ; Fosum, Anna ; Jacobsen, Sten Eirik W LU ; Brundin, Patrik LU and Li, Jia-Yi LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- fluorescence-activated cell sorting, microglia macrophage, transdifferentiation, neural stem cells, plasticity, adult hematopoietic stem cells
- in
- Stem Cells
- volume
- 24
- issue
- 6
- pages
- 1594 - 1604
- publisher
- Oxford University Press
- external identifiers
-
- wos:000240639300024
- pmid:16556707
- scopus:33747589401
- ISSN
- 1549-4918
- DOI
- 10.1634/stemcells.2005-0548
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Stem Cell Center (013041110)
- id
- 2fe52bd5-d3f6-44aa-b781-393d1662798b (old id 154425)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16556707&dopt=Abstract
- date added to LUP
- 2016-04-01 15:38:04
- date last changed
- 2023-01-04 18:20:28
@article{2fe52bd5-d3f6-44aa-b781-393d1662798b,
abstract = {{Previous studies of bone marrow-derived stem cell transdifferentiation into neurons have not involved purified cell populations and determined their exact phenotype prior to differentiation. The present study investigates whether highly purified mouse adult hematopoietic stem cells (HSCs), characterized by lineage marker depletion and expression of the cell surface markers Sca1 and c-Kit (Lin(-)Sca1(+) c-Kit(+) [LSK]), can be stimulated to adopt a neuronal fate. When the HSCLSK cells were cultured in vitro in neuronal differentiation medium supplemented with retinoic acid, 50% of the cells expressed the neural progenitor marker nestin and no cells had become postmitotic. Electrophysiological recordings on neuron-like cells showed that these cells were incapable of generating action potentials. When the HSCLSK cells either were grown in vitro together with neural precursor cells or were transplanted into the striatum or cerebellum of wild-type mouse, they either differentiated into Iba1-immunopositive macrophage/microglia or died. In conclusion, we demonstrate that adult HSCLSK cells do not have the capacity to leave the hematopoietic lineage and differentiate into neurons.}},
author = {{Roybon, Laurent and Ma, Zhi and Asztely, Fredrik and Fosum, Anna and Jacobsen, Sten Eirik W and Brundin, Patrik and Li, Jia-Yi}},
issn = {{1549-4918}},
keywords = {{fluorescence-activated cell sorting; microglia macrophage; transdifferentiation; neural stem cells; plasticity; adult hematopoietic stem cells}},
language = {{eng}},
number = {{6}},
pages = {{1594--1604}},
publisher = {{Oxford University Press}},
series = {{Stem Cells}},
title = {{Failure of transdifferentiation of adult hematopoietic stem cells into neurons.}},
url = {{http://dx.doi.org/10.1634/stemcells.2005-0548}},
doi = {{10.1634/stemcells.2005-0548}},
volume = {{24}},
year = {{2006}},
}