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Reagentless Amperometric Biosensor For Nadh Detection

Muresan, Laura LU ; Nistor, Mihaela LU ; Csöregi, Elisabeth LU and Popescu, Catalin (2009) In Revue Roumaine de Chimie 54(9). p.755-760
Abstract
A sensitive and selective amperometric biosensor, based on NADH-oxidase (NADOx) working in tandem with horseradish peroxidase (HRP), which is electrically connected to graphite electrode via Os-redox polymer (RP), was developed. Two designs were considered for the biosensor optimization: (i) the first one, [G/HRP-NADOx], exploited the direct electron transfer between HRP and the graphite electrode (G); (ii) the second one, [G/RP-HR-P-NADOx], used R-P as mediator. In both cases the enzyme matrix was cross-linked using poly(ethylenglycol) diglycidyl ether. The biosensors sensitivities, estimated as the slopes of linear ranges from the calibration curves, recorded in a single line flow injection setup, showed that G/RP-HRP-NADOx electrode... (More)
A sensitive and selective amperometric biosensor, based on NADH-oxidase (NADOx) working in tandem with horseradish peroxidase (HRP), which is electrically connected to graphite electrode via Os-redox polymer (RP), was developed. Two designs were considered for the biosensor optimization: (i) the first one, [G/HRP-NADOx], exploited the direct electron transfer between HRP and the graphite electrode (G); (ii) the second one, [G/RP-HR-P-NADOx], used R-P as mediator. In both cases the enzyme matrix was cross-linked using poly(ethylenglycol) diglycidyl ether. The biosensors sensitivities, estimated as the slopes of linear ranges from the calibration curves, recorded in a single line flow injection setup, showed that G/RP-HRP-NADOx electrode (1.62 +/- 0.05 mA M-1) presents a higher efficiency than G/HRP-NADOx electrode (0.30 +/- 0.03 mA M-1). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
NADH detection, amperometric biosensors, NADOx, Os-redox polymer
in
Revue Roumaine de Chimie
volume
54
issue
9
pages
755 - 760
publisher
Romanian Academy / Academia Române
external identifiers
  • wos:000273850000007
  • scopus:75949091709
ISSN
0035-3930
language
English
LU publication?
yes
id
a459b5cb-a42f-4d86-b869-5f59c575e1a7 (old id 1546486)
date added to LUP
2010-02-25 15:37:50
date last changed
2017-01-01 06:00:28
@article{a459b5cb-a42f-4d86-b869-5f59c575e1a7,
  abstract     = {A sensitive and selective amperometric biosensor, based on NADH-oxidase (NADOx) working in tandem with horseradish peroxidase (HRP), which is electrically connected to graphite electrode via Os-redox polymer (RP), was developed. Two designs were considered for the biosensor optimization: (i) the first one, [G/HRP-NADOx], exploited the direct electron transfer between HRP and the graphite electrode (G); (ii) the second one, [G/RP-HR-P-NADOx], used R-P as mediator. In both cases the enzyme matrix was cross-linked using poly(ethylenglycol) diglycidyl ether. The biosensors sensitivities, estimated as the slopes of linear ranges from the calibration curves, recorded in a single line flow injection setup, showed that G/RP-HRP-NADOx electrode (1.62 +/- 0.05 mA M-1) presents a higher efficiency than G/HRP-NADOx electrode (0.30 +/- 0.03 mA M-1).},
  author       = {Muresan, Laura and Nistor, Mihaela and Csöregi, Elisabeth and Popescu, Catalin},
  issn         = {0035-3930},
  keyword      = {NADH detection,amperometric biosensors,NADOx,Os-redox polymer},
  language     = {eng},
  number       = {9},
  pages        = {755--760},
  publisher    = {Romanian Academy / Academia Române},
  series       = {Revue Roumaine de Chimie},
  title        = {Reagentless Amperometric Biosensor For Nadh Detection},
  volume       = {54},
  year         = {2009},
}