Reagentless Amperometric Biosensor For Nadh Detection
(2009) In Revue Roumaine de Chimie 54(9). p.755-760- Abstract
- A sensitive and selective amperometric biosensor, based on NADH-oxidase (NADOx) working in tandem with horseradish peroxidase (HRP), which is electrically connected to graphite electrode via Os-redox polymer (RP), was developed. Two designs were considered for the biosensor optimization: (i) the first one, [G/HRP-NADOx], exploited the direct electron transfer between HRP and the graphite electrode (G); (ii) the second one, [G/RP-HR-P-NADOx], used R-P as mediator. In both cases the enzyme matrix was cross-linked using poly(ethylenglycol) diglycidyl ether. The biosensors sensitivities, estimated as the slopes of linear ranges from the calibration curves, recorded in a single line flow injection setup, showed that G/RP-HRP-NADOx electrode... (More)
- A sensitive and selective amperometric biosensor, based on NADH-oxidase (NADOx) working in tandem with horseradish peroxidase (HRP), which is electrically connected to graphite electrode via Os-redox polymer (RP), was developed. Two designs were considered for the biosensor optimization: (i) the first one, [G/HRP-NADOx], exploited the direct electron transfer between HRP and the graphite electrode (G); (ii) the second one, [G/RP-HR-P-NADOx], used R-P as mediator. In both cases the enzyme matrix was cross-linked using poly(ethylenglycol) diglycidyl ether. The biosensors sensitivities, estimated as the slopes of linear ranges from the calibration curves, recorded in a single line flow injection setup, showed that G/RP-HRP-NADOx electrode (1.62 +/- 0.05 mA M-1) presents a higher efficiency than G/HRP-NADOx electrode (0.30 +/- 0.03 mA M-1). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1546486
- author
- Muresan, Laura LU ; Nistor, Mihaela LU ; Csöregi, Elisabeth LU and Popescu, Catalin
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- NADH detection, amperometric biosensors, NADOx, Os-redox polymer
- in
- Revue Roumaine de Chimie
- volume
- 54
- issue
- 9
- pages
- 755 - 760
- publisher
- Romanian Academy / Academia Române
- external identifiers
-
- wos:000273850000007
- scopus:75949091709
- ISSN
- 0035-3930
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biotechnology (LTH) (011001037), Analytical Chemistry (S/LTH) (011001004)
- id
- a459b5cb-a42f-4d86-b869-5f59c575e1a7 (old id 1546486)
- date added to LUP
- 2016-04-01 14:01:01
- date last changed
- 2022-01-27 22:22:34
@article{a459b5cb-a42f-4d86-b869-5f59c575e1a7, abstract = {{A sensitive and selective amperometric biosensor, based on NADH-oxidase (NADOx) working in tandem with horseradish peroxidase (HRP), which is electrically connected to graphite electrode via Os-redox polymer (RP), was developed. Two designs were considered for the biosensor optimization: (i) the first one, [G/HRP-NADOx], exploited the direct electron transfer between HRP and the graphite electrode (G); (ii) the second one, [G/RP-HR-P-NADOx], used R-P as mediator. In both cases the enzyme matrix was cross-linked using poly(ethylenglycol) diglycidyl ether. The biosensors sensitivities, estimated as the slopes of linear ranges from the calibration curves, recorded in a single line flow injection setup, showed that G/RP-HRP-NADOx electrode (1.62 +/- 0.05 mA M-1) presents a higher efficiency than G/HRP-NADOx electrode (0.30 +/- 0.03 mA M-1).}}, author = {{Muresan, Laura and Nistor, Mihaela and Csöregi, Elisabeth and Popescu, Catalin}}, issn = {{0035-3930}}, keywords = {{NADH detection; amperometric biosensors; NADOx; Os-redox polymer}}, language = {{eng}}, number = {{9}}, pages = {{755--760}}, publisher = {{Romanian Academy / Academia Române}}, series = {{Revue Roumaine de Chimie}}, title = {{Reagentless Amperometric Biosensor For Nadh Detection}}, volume = {{54}}, year = {{2009}}, }